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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 939-489-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study meets generally accepted scientific principles (IHT; restriction: purity unknown, limited documentation, no determination of test atmosphere concentration). The registered substance is a reaction product of 2,4-dinitrotoluene and 2,6-dinitrotoluene and hydrogen (common name: diaminomethylcyclohexane or MDACH, respectively) consisting of the two isomers 4 -methylcyclohexane-1,3-diamine (CAS 13897-55-7; molecular weight approx. 128 g/mol) and 2-methylcyclohexane-1,3-diamine (CAS 13897-56-8; molecular weight approx. 128 g/mol). The registered substance was tested in several toxicity studies, which were conducted on the one hand using 4-methylcyclohexane-1,3-diamine (CAS 13897-55-7) which is the main isomer of the registered substance (up to 90%) and on the other hand the reaction product of 2,4-dinitrotoluene and 2,6-dinitrotoluene and hydrogen was tested as such (for details see read across justification, IUCLID chapter 13).
Data source
Reference
- Reference Type:
- publication
- Title:
- Range-Finding Toxicity Data: List VII
- Author:
- Smyth HF, Carpenter CP, Weil CS, Pozzani UC, Striegel JA, Nycum JS
- Year:
- 1 969
- Bibliographic source:
- Am. Ind. Hyg. Assoc. J. 30: 470-476
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Inhalation hazard test: to evaluate the acute inhalation toxicity, group of male Carworth-Wistar rats were exposed for 8 hours to an atmosphere saturated with vapours of the test substance and observed 14 days for mortality.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-methylcyclohexane-1,3-diamine
- EC Number:
- 237-666-9
- EC Name:
- 4-methylcyclohexane-1,3-diamine
- Cas Number:
- 13897-55-7
- Molecular formula:
- C7H16N2
- IUPAC Name:
- 4-methylcyclohexane-1,3-diamine
- Details on test material:
- - Name of test material (as cited in study report): 2,4-diamino-1-methylcyclohehexane
No additional details provided
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Sherman
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: usually 100-150 g
ENVIRONMENTAL CONDITIONS
not reported
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Sealed chamber
- Exposure chamber volume: 120-liter
- Method of holding animals in test chamber: Six rats are rapidly introduced by means of a drawer-type cage designed to minimize vapor loss.
- Source and rate of air: For inhalation periods of ten, five and two minutes in duration, a static technique is used whereby 50 to 100 grams of material, spread over a shallow tray 200 square inches in area, is placed in a 120-liter sealed chamber for at least 24 hours.
- Method of conditioning air: The vapor-air mixture is generated by passing 2.5 liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle.
The exposure apparatus is described in sufficient detail in Smyth et al. 1949, "Assay for Acute Vapor Toxicity". - Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- 8 h
- Concentrations:
- Not determined (IHT)
- No. of animals per sex per dose:
- 6
- Control animals:
- not specified
- Details on study design:
- Animals were weighed prior to exposure and at the end of the post-exposure 14-day observation interval. Animals were observed for signs of toxicity during and after the exposure interval, and daily during the 14-day postexposure observation interval.
Autopsies are performed on all of the rats to ascertain that they did not die of extraneous infection. Gross pathologic change is recorded and liver, spleen, adrenal gland, kidney, and testis are taken for histopathological interpretation from 2 of the rats which died during the 14 day observation period and also from 2 that survived. The survivors are weighed and killed by transecting the spinal cord in the cervical region. An attempt is made to take the tissues for histopathological study from rats exposed to the concentration which killed either 2, 3, or 4 of the 6 exposed. - Statistics:
- The LC50 value was calculated ex post with a probit analysis according to Finney.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Based on:
- test mat.
- Exp. duration:
- 8 h
- Remarks on result:
- other: No death occurred when the test animals were exposed to an atmosphere saturated with vapours of test substance (IHT)
- Mortality:
- No death occurred
- Clinical signs:
- other: Not reported in the publication.
- Body weight:
- Not reported in the publication.
- Gross pathology:
- Not reported in the publication.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.