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EC number: 800-182-9 | CAS number: 1426148-68-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-08-22 to 2012-08-23
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Alcohols, C6-C8-(even numbered, linear)-ethoxylated (<2,5 EO)
- EC Number:
- 800-182-9
- Cas Number:
- 1426148-68-6
- Molecular formula:
- R-O-R' , whereas R=C6-8-(even numbered, linear, saturated)-alkyl and R'=H or xEO wheras x=1-12, mean=2,3
- IUPAC Name:
- Alcohols, C6-C8-(even numbered, linear)-ethoxylated (<2,5 EO)
- Details on test material:
- - Name of test material (as cited in study report): Alcohols, C6-8 alkyl-(even, linear), ethoxylated (< 2.5 EO)
- Physical state: Liquid, colourless
- Analytical purity: 90 % (w/w)
- Purity test date: 2012-06-15
- Lot/batch No.: ESD0006420 (19.05.2011)
- Expiration date of the lot/batch: 2013-09-01
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 5 g/L was prepared and agitated.
- Eluate: Synthetic waste water according to OECD Guidelinie 209
- Differential loadings: 10 - 32 - 100 - 320 - 1000 mg/L
- Controls: Test medium without test or reference item.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: Washed twice with chlorine free tap water. Adjustment to a dry sludge concentration of 3.0 g/L ± 10 % was not necessary. The sludge was used within 24 h after sampling.
- Dry sludge concentration: 2.94 g/L, corresponding to 1.47 g/L in the test vessel
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 19.6 °C
- pH:
- pH-value of the activated sludge 7.88
pH-value of the synthetic waste water 7.55 - Salinity:
- Not determined
- Nominal and measured concentrations:
- 10 - 32 - 100 - 320 - 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask
- Aeration: Shaking of flasks at 150 rpm to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Composition of test medium:
Test item concentration [mg/L]
10 32 100 320 1000
Inoculum [mL] 250
Synthetic waste water [mL] 16
Stock solution [mL] 1.0 3.2 10 32 100
Demineralised water [mL] 233 230.8 224 202 134
Test / reference item (direct weighing)
Reference item concentration [mg/L]
10 32 1000
Inoculum [mL] 250
Synthetic waste water [mL] 16
Stock solution [µL] 97 167 300
Demineralised water [mL] 233.9 233.83 233.7
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter immediately after transfering the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flatbed recorder.
TEST CONCENTRATIONS
- Range finding studies:
Inhibition of Respiration (Preliminary Tests)
Nominal Test Item Concentration pH pH Inhibition [%]
[mg/L] (test item in water) (test item in medium)
1 - - -1
10 - - 1
100 - - 2
10 - - 7
100 - - 6
1000 - - 63
1000 (6.29)
7.80* 7.77 65 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 618 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI (575-661 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 114 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC-values of the test item and the reference item were calculated by linear regression using software GraphPadPrism. Calculations of the confidence intervals for the EC-values were carried out using standard procedures.
Any other information on results incl. tables
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h.
The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison with the oxygen uptake rates of the control.
Oxygen Uptake Rates, Specific
Respiration Rates and Inhibition of the Respiration
of the Control, Test and Reference Item Concentrations
Test Item |
Repl. |
Oxygen Uptake Rate |
Specific Respiration Rate Rs |
Inhibition |
Mean Inhibition |
|
[mg/L] |
||||||
Control |
1 |
26.8 |
18.2 |
– |
– |
|
2 |
25.6 |
17.4 |
||||
3 |
28.8 |
19.6 |
||||
4 |
28.4 |
19.3 |
||||
5 |
29.2 |
19.9 |
||||
6 |
27.2 |
18.5 |
||||
Test Item |
10 |
1 |
28.0 |
-1 |
-3 |
|
2 |
28.8 |
-4 |
||||
3 |
27.6 |
0 |
||||
4 |
29.6 |
-7 |
||||
5 |
28.0 |
-1 |
||||
32 |
1 |
28.8 |
-4 |
-7 |
||
2 |
29.2 |
-5 |
||||
3 |
30.8 |
-11 |
||||
4 |
30.4 |
-10 |
||||
5 |
29.6 |
-7 |
||||
100 |
1 |
30.4 |
-10 |
-4 |
||
2 |
27.6 |
0 |
||||
3 |
28.8 |
-4 |
||||
4 |
28.0 |
-1 |
||||
5 |
29.2 |
-5 |
||||
320 |
1 |
32.8 |
-18 |
-19 |
||
2 |
31.6 |
-14 |
||||
3 |
33.6 |
-21 |
||||
4 |
34.8 |
-26 |
||||
5 |
32.0 |
-16 |
||||
1000 |
1 |
19.6 |
29 |
31 |
||
2 |
19.2 |
31 |
||||
3 |
19.2 |
31 |
||||
4 |
18.4 |
34 |
||||
5 |
19.2 |
31 |
||||
Reference Item |
58 |
1 |
24.4 |
12 |
15 |
|
2 |
23.6 |
15 |
||||
3 |
22.4 |
19 |
||||
100 |
4 |
16.0 |
42 |
42 |
||
5 |
-# |
- |
||||
2 |
16.0 |
42 |
||||
180 |
3 |
7.2 |
74 |
74 |
||
4 |
7.2 |
74 |
||||
5 |
6.8 |
75 |
Coefficient of variation of oxygen uptake rates of the control: 5.0 %
Suspended
solids in the test vessel: 1.47 g/L
#) Replicate not measured, Erlenmeyer flask broke before
measurement
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC10 of Alcohols, C6-8 alkyl-(even, linear), ethoxylated (<2.5 EO) is 618 mg/L. Alcohols, C6-8 alkyl-(even, linear), ethoxylated (<2.5 EO) is not toxic up to the concentration of 618 mg/L to activated sludge of a municipal sewage treatment plant. The EC20 of the active ingredient is 776 mg/L, the EC50 and the EC80 are > 1000 mg/L.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Alcohols, C6-8 alkyl-(even, linear), ethoxylated (2.5 EO)(batch number: ESD0006420) from 2012-08-22 to 2012-08-23, with the definitive exposure on 2012-08-23 at the Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the substance concentrations 10 - 32 - 100 - 320 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -19 % to 31 % .
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 114 mg/L.
NOEC and EC-Values with Confidence Interval of Alcohols, C6-8 alkyl-(even, linear), ethoxylated (2.5 EO)
EC-values
[mg/L]Confidence interval
P = 95 [%]
[mg/L]NOEC*
320
–
EC10
618
575 - 661
EC20
776
724 - 851
EC50
> 1000
–
EC80
> 1000
–
The EC10 of Alcohols, C6-8 alkyl-(even, linear), ethoxylated (2.5 EO) is 618 mg/L.
The test item is not toxic up to the concentration of 618 mg/L to activated sludge
of a municipal sewage treatment plant. The EC50 is > 1000 mg/L.
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