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EC number: 695-595-2 | CAS number: 83395-38-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Oct. 2021-27 Jan. 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- adopted 23. Mar. 2006, Annex 5 corrected: 28 July 2011
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EU) No. 2016/266 amending Regulation (EC) No. 440/2008, Annex IV, Method C.3: “FRESHWATER ALGAE AND CYANOBACTERIA, GROWTH INHIBITION TEST."
- Version / remarks:
- adopted 07. December 2015
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-yn-1-yl 1H-imidazole-1-carboxylate
- EC Number:
- 695-595-2
- Cas Number:
- 83395-38-4
- Molecular formula:
- C7H6N2O2
- IUPAC Name:
- prop-2-yn-1-yl 1H-imidazole-1-carboxylate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Name: 2-propyn-1-yl 1H-imidazole-1-carboxylate
Batch no.: 0802-2#
CAS no.: 83395-38-4
Storage: fridge (2 - 8 °C) under inert gas
Expiry date: 21. Mar. 2022
Appearance: white solid
Purity: 99.1 %
Homogeneity: homogeneous
Production date: 13. Oct. 2020
EC no.: 695-595-2
Molecular formula: C7H6N2O2
Molecular weight: 150.13 g/mol
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 4.6 / 10 / 22 / 46 / 100 mg/L nominal concentration
- Sample storage conditions before analysis: stored in a closed vessel at room temperature (20 ± 5 °C)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Positive Control: Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Blank Control: 350 mL nutrient medium
- Stock solution containing 100 mg/L test item in algal medium (demineralised water en-riched with minerals but without algae) was prepared. Lower test concentrations were prepared by dilution of the stock solution in algal medium.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- SAG Strain Number: 86.81
- Taxonomic position: Chlorophyta - Chlorophyceae
- Origin and Culture: The culture of Desmodesmus subspicatus was obtained in May 2020 by MBM Sciencebridge GmbH. The algae are kept as stock cul-ture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.6-22.5 °C
- pH:
- 7.5-7.6
- Nominal and measured concentrations:
- Nominal concentration: 4.6/ 10 / 22 / 46 / 100 mg/L
Measured concentration: 6.19 / 13.20 / 28.64 / 60.59 and 136.72 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks total volume 65 mL
- Initial cells number: 2880 Cell Number/mL
- Control end cells number: 320030 Cell Number/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralised water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Light intensity and quality: within the specified range (4440 – 8880 Lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
- observation intervals: every 24 ± 1 hours - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.85 mg/L
- 95% CI:
- 7.58 - 20.13
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.92 mg/L
- 95% CI:
- 0.75 - 1.17
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.32 mg/L
- 95% CI:
- 0.14 - 0.45
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.34 mg/L
- 95% CI:
- 0.09 - 0.75
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- <= 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- <= 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study, the 72 hour ErC50 of the test material was determined to be 11.85 mg/L.
- Executive summary:
In this guideline (OECD 201) study conducted with GLP certification, the objective of this study was to evaluate the toxicity of 2-propyn-1-yl 1H-imidazole-1-carboxylate towards Desmodesmus subspicatus.
The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L (nominal). Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at all concentrations.
At the start, after 24 h and after 48 h the content of the test item in the test solutions was determined using HPLC determination. At the beginning of the test, the measured concentrations lay between 130 % and 137 % of the nominal concentrations. Because the test item was not stable under test conditions, after 24h the test item was still detectable only in the two highest concentrations 46 mg/L sand 100 mg/L. Because after 48 h no test item was detectable, no further measurement after 72 h was performed. Therefore, the determination of the results was based on the geometric mean of the measured concentrations. Geometric mean is calculated by multiplication of the n participating concentrations and taking the nth root.
For the three lowest concentrations 4.6 mg/L, 10 mg/L and 22 mg/L, the measured concentration at the beginning, the half of the LOQ (0.05 mg/L) was used for calculation of the geometric mean.
The 72h-EC50 values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.64 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test item 2-propyn-1-yl 1H-imidazole-1-carboxylate were determined:
Table: Results of the test item
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
< 0.56 mg/L
≤ 0.56 mg/L
0.34 mg/L
(extrapolated)
11.85 mg/L
Yield
< 0.56 mg/L
≤ 0.56 mg/L
0.32 mg/L
(extrapolated)
0.92 mg/L
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