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EC number: 684-879-1 | CAS number: 848641-69-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Chemicals - Daphnia magna acute immobilisation test
- Version / remarks:
- GB/T 21830-2008
- Qualifier:
- according to guideline
- Guideline:
- other: The Guidelines for the testing of chemicals-effects on biotic systems
- Version / remarks:
- 2nd edition, Beijing: China Environment Press, 2013
- Qualifier:
- according to guideline
- Guideline:
- other: The guidelines for the testing of chemicals
- Version / remarks:
- HJ/T 153-2004
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Version of 2004
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-Ethyl-3-methylimidazolium Diethyl Phosphate
- Cas Number:
- 848641-69-0
- Molecular formula:
- C6H11N2 C4H10O4P
- IUPAC Name:
- 1-Ethyl-3-methylimidazolium Diethyl Phosphate
- Test material form:
- liquid
- Details on test material:
- Batch No 187-9-113-Y
Expiry date: 2021-1-30
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 187-9-113-Y
- Expiration date of the lot/batch: 2021-01-30
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Keep the container closed and in a dry, cool, well ventilated area
- Stability under test conditions:
- Solubility and stability of the test substance in the test medium: good solublility; analysed concentration was maintained above 80% of the initial measured concentration
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 100 mg/L
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Study was performed as a limit test at a concentration of 100 mg/L. Sampling was done at 0 hours, 24 hours and after 48 hours. Analysis was done by UPLC-MS.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution (100 mg/L) was prepared by dissolving 0.0256 g of the test substance in 250 ml Elendt M7 medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna was introduced from Nanjing Institute of Environmental Science, MEP in 2018. Culture medium was Elendt M7. All organisms used for a test originated from cultures established from the same healthy stock of Daphnia. The animals were maintained under culture conditions (light, temperature and medium) similar to those used in the test. Animals used for the test were not older than 24 hours.
- Age at study initiation: <24 hours
- Feeding during test: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.2°C to 20.4°C
- pH:
- in the range of 6.87 to 7.2 without adjustment of pH during the limit test
- Dissolved oxygen:
- 8.09 mg/L to 8.54 mg/L of the air saturation without aerating
- Nominal and measured concentrations:
- nominal: 100 mg/L
measured:
0h 106 mg/L
24h 104 mg/L
48h 101 mg/L
mean measured concentration: 104 mg/L - Details on test conditions:
- 4 replicates with 5 daphnids in 50 ml of test solution were used in the main test, open test vessels were used and no aeration was provided. The control also consists of 4 replicates with 5 daphnids each.
Photoperiod: 16:8 light-dark cycle
Number of immobilised daphnids was determined after 24 hours and 48 hours.
RANGE-FINDING STUDY
- Test concentrations: 1 mg/L, 10 mg/L and 100 mg/L
- Results used to determine the conditions for the definitive study: no immobilised daphnids were observed therefore the main study was conducted as a limit test with 100 mg/L test substance. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 (24h) with K2Cr2O7 was 1.46 mg/L
- Reported statistics and error estimates:
- EC50 and 95% confidence intervals were calculated by Trimmed Spearman-Karber Method (Version 1.5, US EPA)
Any other information on results incl. tables
see attachments
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is not toxic to daphnids under static conditions in a limit test with a concentration of 100 mg/L.
- Executive summary:
The toxicity of the test item to Daphnia magna was investigated in a limit test with a concentration of 100 mg/l according to Chinese chemical law guidelines and OECD 202. The concentration of the test substance was stable over the test duration of 48 hours. The test fulfills the validity criteria:
- Number of immobilised daphnids in the control was <10%
- dissolved oxygen concentration was more than 3 mg/l in control and test vessels
- the pH values did not differ by more than 1.5 units at test start and end
Under the test conditions the 48 h EC50 of the test substance was >100 mg/L.
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