Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-591-4 | CAS number: 4948-28-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 13, 2003 to September 11, 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: 0 mg/L (control), 13, 22, 36, 60, and 100 mg/L
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
A 100 mg/L stock solution was prepared by bringing 0.1004 g of test substance to 1,000 mL with dilution water in a Class A volumetric flask. The solution was mixed on a magnetic stirrer for approximately 2 hours. A series of solutions was then prepared by bringing appropriate volumes of stock solution to 250 mL with dilution water in Class A volumetric flasks. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): less than 24 hours old
- Weight at study initiation (mean and range, SD): average wet weight of 0.18 mg
-Method of breeding: in-house culture
- Source: Aquatic Biosystems Inc., Fort Collins, Colorado, on February 20, 2003
- Feeding during test: no
ACCLIMATION
- Acclimation period: 35 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: freshwater algae, Selenastrum capricornutum, and a mixture of yeast and trout chow
- Feeding frequency: once each day
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 160 to 180 mg/L as CaCO3
- Test temperature:
- 20.0 to 21.0°C (mean = 20.6°C)
- pH:
- 7.0
- Dissolved oxygen:
- from 7.7 to 8.8 mg/L (mean = 8.4 mg/L)
- Conductivity:
- 600 μmhos/cm
- Nominal and measured concentrations:
- Nominal concentrations were: 0 mg/L (control), 13, 22, 36, 60, and 100 mg/L.
Mean measured concentrations were: ND (none detected at or above the limit of quantitation; control), 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 40 mL
- Type: closed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: carbon filtered, deionized water collected at T.R. Wilbury Laboratories in Marblehead, Massachusetts. Water was adjusted to a hardness of 160 to 180 mg/L as CaCO3 and stored in polyethylene tanks where it was aerated and recirculated through particle filters, activated carbon, and an ultraviolet sterilizer. Prior to use, the pH of the dilution water was adjusted to <8.0 with 5% H3PO4.
- Total organic carbon: ND
- Pesticides: ND
OTHER TEST CONDITIONS
- Photoperiod: 16 hour light and 8 hour dark
- Light intensity: 32 footcandles
RANGE-FINDING STUDY
- Test concentrations: 10 mg/L and 100 mg/L
- Results used to determine the conditions for the definitive study: 100% survival in the control, 100% survival at 10 mg/L, and 20% survival at 100 mg/L. - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58.5 mg/L
- 95% CI:
- 51.1 - 66.6
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 72.8 mg/L
- 95% CI:
- 56.7 - 93.6
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 33.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0
- Immobilisation of control: 0 - Conclusions:
- The EC50 of the test item on D. magna was calculated as 58.5 mg/L after 48 h.
- Executive summary:
The accute toxicity of the test item on the aquatic invertebrate Daphnia magna was studied. The test followed the OECD guideline 202, 1984. It was perfomed during 48 hours under static conditions and closed vessels. The test animals were less than 24 hours old. A total of 20 animals were used per each test concentration as it is stated in the guideline. The mean measured concentrations utilized were 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L. Which ranged from 91 to 95% of nominal concentrations. The effective concentrations calculated were a EC50 of 58.5 mg/L, a LC50 of 72.8 mg/L, and a NOEC of 33.5 mg/L
Reference
Description of key information
EC50 (48 h)= 58.5 mg/L (Daphnia magna, OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 58.5 mg/L
Additional information
The accute toxicity of the test item on the aquatic invertebrate Daphnia magna was studied. The test followed the OECD guideline 202, 1984. It was perfomed during 48 hours under static conditions and closed vessels. The test animals were less than 24 hours old. A total of 20 animals were used per each test concentration as it is stated in the guideline. The mean measured concentrations utilized were 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L. Which ranged from 91 to 95% of nominal concentrations. The effective concentrations calculated were a EC50 of 58.5 mg/L, a LC50 of 72.8 mg/L, and a NOEC of 33.5 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.