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Diss Factsheets
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EC number: 201-873-2 | CAS number: 88-99-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Principles of method if other than guideline:
- Method is in most parts equivalent to the OECD TG 201
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The stability of the test substance was experimentally determined
with HPLC before and after an exposure of 72 hours - Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Duration:
- 72 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- results with pH adjustment
- Validity criteria fulfilled:
- yes
- Executive summary:
On Desmodesmus subspicatus two limit tests were conducted each with 100 mg/l with and without pH adjustment. The 72 h-EC0 was >/= 100 mg/l with pH adjustment. The results are related to both endpoints growth rate and biomass (Bayer Industry Services, 2004).
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Principles of method if other than guideline:
- Method: other: Growth inhibition test
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Test organisms (species):
- other: other algae: Scenedesmus sp. and Microcystis sp.
- Test type:
- static
- Water media type:
- freshwater
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: It is not clear whether the algae were within the exponential growth throughout the whole exposure period of 7 days
- Principles of method if other than guideline:
- Method: other: modified to ISO 8692
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Test organisms (species):
- other: other algae: Scenedesmus subspicatus CHODAT
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 258 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Referenceopen allclose all
With pH adjustment, the recovery rate was > 98 %, therefore the nominal value was reported. The result is related to both endpoints growth rate and biomass.
Without pH adjustment at 100 mg/l 100 % inhibition was observed after 72 hours; pH was determined to be in the range of 4.9 to 5.1.
The inhibitory effect on the growth of Scenedesmus sp. of rotted materials (on average):
2-days: 57%
8-days: 49%
14-days: 41%
21-days: 42%
For Microcystis sp., an antialgal effect of the 2-day rotted materials of 93% (on average) is reported.
The antialgal effect decreased relative to the rotting time flow for both algae.
One of the inhibitory chemicals, released from the rotted plants, was identified as 1,2-benzenedicarboxylic acid (o-phthalic acid).
Endpoint biomass: 7 d EC50: 506 mg/l RM-Freetext:
Accepted new scientific name for Scenedesmus subspicatus:
Desmodesmus subspicatus.
Description of key information
On Desmodesmus subspicatus two limit tests were conducted each with 100 mg/l with and without pH adjustment. The 72 h-EC0 was >/= 100 mg/l with pH adjustment. The results are related to both endpoints growth rate and biomass (Bayer Industry Services, 2004).
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Algal toxicity was determined by a GLP test with Desmodesmus subspicatus in the presence of phthalic acid according to the EU Method C.3 "Algal Inhibition Test" wich is in most parts equivalent to the OECD Guideline 201 “Alga Growth Inhibition Test”. Two limit tests were conducted each with 100 mg/l with and without pH adjustment. The stability of the test substance was experimentally determined via HPLC before and after an exposure of 72 hours. The 72 h-EC0 was >/= 100 mg/l and the recovery rate was > 98 %, therefore the nominal value was reported. The results are related to both endpoints growth rate and biomass. Without pH adjustment at 100 mg/l 100 % inhibition was observed after 72 hours; pH was determined to be in the range of 4.9 to 5.1. This indicates that phthalic acid causes a pH shift in algal medium. It is the resulting pH that determines the impact of phthalic acid on algae as shown with buffered test substance solution. Thus, toxic effects are not due to substance inherent properties but due to a function of the pH (Bayer Industry Services, 2004).
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