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Diss Factsheets
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EC number: 605-350-3 | CAS number: 16397-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- p-Nitrobenzoic acid-2-ethylhexylester
- EC Number:
- 605-350-3
- Cas Number:
- 16397-70-9
- Molecular formula:
- C15 H21 N O4
- IUPAC Name:
- p-Nitrobenzoic acid-2-ethylhexylester
- Details on test material:
- Name of the test substance used in the study report: p-Nitrobenzoesaeure-2-ethylhexylester
Purity: 99.5% (GC)
- Physical state: LIQUID, LIGHT YELLOW
- Lot/batch No.: 22 170/85
- Stability under test conditions: WAS CONFIRMED BY ANALYSIS
- Storage condition of test material: ROOM TEMPERATURE
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- The animals were housed in stainless steel wire mesh cages with no bedding in the cages (sawdust in the waste trays). Identification of the rats via cage cards and tail marking. The animals were housed in fully air-conditioned rooms with central air-conditioning in a range of 20 - 24°C for temperature and 30 - 70% for relative humidity. The day/night rhythm was 12 h dark and 12 h light. A standardized laboratory diet was offered; drinking water (tap water) was available ad libitum.
Young adult rats of a comparable weight were used: males 190-199g; females 170-181g
ANIMAL BREEDER: DR. K. THOMAE GMBH, Germany
ACCLIMATIZATION PERIOD: AT LEAST 1 WEEK
FASTING PERIOD: ABOUT 16 HOURS BEFORE ADMINISTRATION (WATER WAS AVAILABLE AD LIBIDUM)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 5 ml/kg - Doses:
- 681, 1470, 2200 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
RECORDING OF SIGNS AND SYMPTOMS SEVERAL TIMES ON THE DAY OF ADMINISTRATION. AT LEAST ONCE EACH WORKDAY. CHECK FOR MORIBUND AND DEAD ANIMALS TWICE EACH WORKDAY AND ONCE ON HOLIDAYS.
Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 200 mg/kg bw
- Mortality:
- Male animals: no mortalities
Female animals: 1 animal died within day1 - Clinical signs:
- Male animals: poor general state, dyspnoea, apathy, staggering, piloerection.
Female animals: poor general state, dyspnoea, apathy, abdominal position, paresis, piloerection.
All findings declined after day 2. - Body weight:
- Male animals - before application: 199 g (low dose); 190 g (mid dose); 198 g (high dose).
Male animals - after 13 days: 303 g (low dose); 295 g (mid dose); 297 g (high dose).
Female animals - before application: 170 g (low dose); 181 g (mid dose); 178 g (high dose).
Female animals - after 13 days: 213 g (low dose); 226 g (mid dose); 220 g (high dose). - Gross pathology:
- Animal that died (2200 mg/kg): general congestion; liver: gray-brown coloured.
Sacrificed animals: No pathologic findings noted.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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