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Diss Factsheets
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EC number: 941-484-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- EC Number:
- 941-484-1
- Molecular formula:
- not possible
- IUPAC Name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81 (formerly Scenedesmus subspicatus CHODAT)
- Source (laboratory, culture collection): 'Sammlung von Algenkurturen', Göttingen (SAG), (Collection of Algae Cultures, Institute for Plant Physiology), University of Göttingen, Germany
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE·m-2 x s-1 for 24 h per day.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- This medium has a nominal hardness of 0.24 mmol Ca+Mg/L.
- Test temperature:
- Nominal range: 21 - 24 °C, controlled at ± 2 °C
- pH:
- 7.80 - 8.47
- Nominal and measured concentrations:
- nominal (mg/L): 4.00, 1.6, 0.64, 0.256, 0.102
The measured test item concentrations were all within ± 20 % of the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all Sterile Erlenmeyer flasks of 250 mL volume filled with 100 mL test medium
- Aeration: no
- Initial cells density: Nominal: approximately 5 x 10^3 - 10^4 cells/mL Current: 4998 cells/mL
- Control end cells density (mena): 551691 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: Nutrient medium Z according to LÜTTGE et al. (1994)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: double distilled water
- Total organic carbon: NaHCO3 50 mg/L
- Ca/mg ratio:of 0.24 mmol Ca+Mg/L
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no. The pH-value at the beginning of the test was measured in one additional replicate per concentration level and control. At the test end, it was measured from pooled replicates per test item concentration and control, respectively.
Photoperiod: 24 hours/day light
- Light intensity: 60 - 120 µE · m-2 · s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): algal cell concentration determined by photometric measurement after 0, 24, 48 and 72 h
- Determination of cell concentrations: Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: The measured test item concentrations were all within ± 20 % of the nominal concentrations.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.611 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % confidence interval: 0.523-0.713
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % confidence interval: 0.137-1.54
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.256 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.436 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95 % confidence interval: 0.274-0.586
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.679 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95 % confidence interval: 0.469-1.08
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.256 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- - Observation of abnormalities (for algal test): unusual observations were not made
- Any stimulation of growth found in any treatment: growth and yield of the algae was increased at concentrations of 0.256 mg/L
- Any observations that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.586 mg/L (growth rate)
Any other information on results incl. tables
The validation criteria were fulfilled as algal growth in the control between 0 and 72 h exceeded the factor of 16 as requested by the underlying OECD guideline.With the current test it was found to be 111. The mean coefficient of variation of the section-by-section (0 - 24, 24 - 48 and 48 – 72 h) growth rate in the control cultures must not exceed 35% and it was 17.5% in the current test.
The coefficient of variation of the average specific growth rate in the control cultures, measured during the exposure period (0-72 h) must not exceed 7% and it was 3.81% in the current test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, the test item was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 hours with the
following effect values: The NOEC-value for growth rate after 72 hours was 0.256 mg/L. The EC50-value for inhibition of growth rate (ErC50) with 95 % confidence intervals after 72 hours was determined to be 1.45 (1.37 – 1.54) mg/L.
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