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EC number: 696-318-8 | CAS number: 174489-43-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- prop-2-en-1-yl 2-(5-amino-2-chlorobenzoyloxy)-2-methylpropanoate
- EC Number:
- 696-318-8
- Cas Number:
- 174489-43-1
- Molecular formula:
- C14H16ClNO4
- IUPAC Name:
- prop-2-en-1-yl 2-(5-amino-2-chlorobenzoyloxy)-2-methylpropanoate
Constituent 1
Method
- Target gene:
- uvrA and uvrB
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Details on mammalian cell type (if applicable):
- Controls of the genotype of the strains:
The characteristics of the strains were checked every two months. Histitidine-auxtorophy of the Salmonella strains was demonstrated by the requirement for L-histidine. The presence of the rfa character was assayed by the sensitivity for crystal-violet. The deletion of the uvrB gene was demonstrated by the sensitivity for UV-light. The Salmonella strains containing the R-factor (TA98 and TA100) were additionally checked for ampicillin resistance. The tryptophan-auxotrophy of E. coli WP2uvrA was demonstrated by the requirement for tryptophan. The absence of the uvrA gene was demonstrated by the sensitivity of the strain for UV-light. Furthermore, all strains were checked for their reversion properties with known mutagens (positive controls).
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- Controls of the genotype of the strains:
The characteristics of the strains were checked every two months. Histitidine-auxtorophy of the Salmonella strains was demonstrated by the requirement for L-histidine. The presence of the rfa character was assayed by the sensitivity for crystal-violet. The deletion of the uvrB gene was demonstrated by the sensitivity for UV-light. The Salmonella strains containing the R-factor (TA98 and TA100) were additionally checked for ampicillin resistance. The tryptophan-auxotrophy of E. coli WP2uvrA was demonstrated by the requirement for tryptophan. The absence of the uvrA gene was demonstrated by the sensitivity of the strain for UV-light. Furthermore, all strains were checked for their reversion properties with known mutagens (positive controls).
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat-liver post mitochondrial supernatant (S9 fraction).
- Test concentrations with justification for top dose:
- Concentration range in the range-finding test:
20.6 to 5000.0 µg/plate
Concentration ranges in the Mutagenicity Tests
Original experiment: 312.5 to 5000.0 µg/plate
Confirmatory experiment with activation:
S. typhimurium: 78.1 to 1250.0 µg/plate
E.coli WP2 uvrA: 312.5 to 5000.0 µg/plate
Confirmatory experiment without activation:
S. typhimurium TA 100, TA 102, TA 98, TA 1537: 32.5 to 1000.0 µg/plate
S. typhimurium TA 1535 and E. coli WP2 uvrA: 312.5 to 5000.0 µg/plate - Vehicle / solvent:
- Dimethylsulphoxide (DMSO)
Controls
- Untreated negative controls:
- yes
- Remarks:
- the solvent alone was used as a negative control
- Negative solvent / vehicle controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- cyclophosphamide
- mitomycin C
- other: 2-Aminoanthracene, 4-Nitroquinoline, Aroclor 1254, Dimethylsulphoxide
- Details on test system and experimental conditions:
Solubilisation of the test substance:
CA 2219 A was dissolved on DMSO at room temperature. The test substance was soluble up to the concentration of 50 mg/L. Lower concentrations of the test substance were obtained by serial dilution of the stock solution with DMSO. No precipitates or aggregates were noted.
Setting up of the plates:
Standard plate incorporation assay: 0.1ml of the overnight cultures were mixed with 2 ml of top agar, either 0.5 ml of 100mM sodium phosphate buffer (experiments without activation) or 0.5 ml of the activation mixture (experiments with activation) and 0.1 ml of a solution of the test substance, the positive control or the solvent as a negative control and poured on minimal agar in Petri dishes.
Preincubation assay: 0.1 ml of the overnight cultures were mixed with 0.5ml of the activation mixture (experiments with activation) and 0.1 ml of a solution of the test substance, the positive control or the solvent as a negative control and incubated for 30 min. at 37°C. Therafter 2 ml of top agar was added to the mixtures and they were poured on minimal agar in Petri dishes.
Each Petri dish contained about 20.0 ml of minimal agar (1.5% agar supplemented with 2% salts of the Vogel-Bonner Medium E and 2% glucose). The top agar was composed of 0.6% agar and 0.6% NaCl. In the experiment with Salmonella the top agar was supplemented with 10% of 0.5 mM L-histidine and 0.5mM d-biotin dissolved in water. In the experiment with E. coli it was supplemented with 10% of 0.5mM L-tryptophan dissolved in water.
Range finding tests:
A range finding test was carried out with strains S. typhimurium TA 100 and E. coli WP2 uvrA with and without metabolic activation at six concentrations of the test substance and one negative control. The highest concentration applied was 5000 µg/plate. The five lower concentrations decreased by a factor of three. The plates were inverted and incubated for about 48 hours at 37 ± 1.5 °C in darkness. Thereafter, they were evaluated by counting the colonies and determining the background lawn. One plate per test substance concentration and negative control was used.
Negative and positive controls
The solvent alone was used as the negative control.
The positive controls (reference mutagens) with and without metabolic activation can be found in Table 1 and Table 2.- Evaluation criteria:
Scoring of the plates
Colonies were counted electronically using Artek Colony Counter (Fisher Scientific), or manually where minor agar damage or test chemical precipitates or strong colouration of the agar plates might have interfered with automatic counting. The results were sent on line to a computer. They were checked on a random basis by the operator. Observations indicating precipitates of the test substance in the top agar or a reduced or absent bacterial background lawn were registered additionally.
Assay acceptance criteria
A test is considered acceptable if the mean colony counts of the negative control values of all strains are within acceptable ranges and if the results of the positive controls meet the criteria for a positive response.
Criteria for a positive response
The test substance will be considered to be positive in the test system if one or both of the following conditions are met:
• At least a reproducible doubling of the mean number of revertants per plate above that of the negative control at any concentration for one or more of the following strains:
TA 98, TA 1535, TA 1537, E.coli WP2 uvrA.
• A reproducible increase of the mean number of revertants per plate for any concentration above that of the negative control by at least a factor of 1.5 for strains TA 100 or TA 102.
Generally a concentration-related effect should be demonstrable.- Statistics:
- A statistical analysis was not performed.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
Range finding tests:
Six concentrations of CA 2219 A ranging from 20.6 to 5000.0 µg/plate were tested with strain Salmonella typhimurium TA 100 and the strain Escherichia coli WP2 uvrA to determine the highest concentration to be used in the mutagenicity assay. The experiment was performed with and without metabolic activation.
Due to a growth inhibiting effect of the test substance in the experiment with metabolic activation on strain TA 100, the number of revertant colonies was reduced at the upper concentrations.
The background growth was invisible at the highest concentration.
In the experiment without activation and on strains TA 100 and E. coli a similar effect occurred at the highest concentration. The background growth was reduced at the highest concentration.
At the concentrations of 5000.0 µg/plate the test substance precipitated in soft agar
From the results obtained the highest concentration suitable for the mutagenicity test was selected to be 312.5 to 5000.0 µg/plate with and without metabolic activation.
Mutagenicity Tests:
In the original and in the performed with and without metabolic activation, treatment of strains TA98, TA 100, TA 102, TA 1535, TA 1537 and WP2 uvrA with CA 2219 A did not lead to an increase in the incidence of either histidine- or tryptophan-prototrophic mutants in comparison with the negative control.
In the confirmatory experiments performed out with and without metabolic activation, again after treatment of strains TA98, TA100, TA102, TA1535, TA 1537 and WP2uvrA with CA 2219 A, no increase in the incidence of either histidine- or tryptophan-prototrophic mutants was observed in comparison with the negative control.
Due to growth inhibiting effect of the test substance a reduction in the number of revertant colonies was visible in the experiments with metabolic activation on all strains of Salmonella tyhimurium at the concentrations of 1250 to 5000 0 µg/plate. In the experiments without activation a reduction in the number of revertant colonies on strains TA 100, TA 102 (1000 to 5000 0 µg/plate), TA 1537 (2500 and 5000 0 µg/plate), TA 98 and E.coli (5000 0 µg/plate).
The growth of the bacterial background lawn was occasionally reduced or invisible at higher concentrations.
At the concentration of 5000 0 µg/plate, the test substance precipitated on the surface of the agar plates.- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Based on the result of these experiments and on standard evaluation criteria, it is concluded that CA 2219 A (Intermediate of CGA 276854) and it’s metabolites did not induce gene mutations in the strains of S. typhimurium and E. coli used. - Executive summary:
CA 2219 A (Intermediate of CGA 276854), identified as a brown liquid with a purity of 95.5%, batch no. 249-GE001/SU, was tested for mutagenic effects in vitro in histidine-requiring strains of Salmonella typhimurium and in a tryptophan-requiring strain of Escherichia coli. The following strains were used: S. typhimurium TA 98, TA 100, TA 102, TA 1535, TA 1537 and E. coli WP2 uvrA. The test was performed with and without the addition of rat-liver post mitochrondrial supernatant (S9 fraction) as an extrinsic metabolic activation system. The compound was dissolved in DMSO and tested at five concentrations in a range of 312.5 to 5000.0 µg/plate in the presence and absence of a metabolic activation system in the original mutagenicity test. In order to confirm the results, the experiment with metabolic activation was repeated on the strain of S. typhimurium at 78.1 to 1250 µg/plate and E. coli at 312.5 to 5000.0 µg/plate. In the repeat experiment without activation on strains TA 98, TA 100, TA 102, TA 1537 the concentrations of 62.5 to 1000.0 µg/plate were tested. The strains TA 1535 and E. coli were tested with the concentrations of 312.5 to 5000.0 µg/plate. Each strain was additionally tested in the presence and in the absence of a metabolic activation system with a suitable, known mutagen as positive control.
The original experiment with and without metabolic activation and the confirmatory experiment without activation were performed as standard plate incorporation assay. The confirmatory experiment with metabolic activation was carried out as preincubation assay.
In both experiments, performed with and without metabolic activation, none of the tested concentrations of CA 2219 A led to an increase in the incidence of either histidine- or tryptophan- prototrophic mutants by comparison with the negative control.
Based on the result of these experiments and on standard evaluation criteria, it is concluded that CA 2219 A (Intermediate of CGA 276854) and it’s metabolites did not induce gene mutations in the strains of S. typhimurium and E. coli used.
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