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EC number: 223-564-1 | CAS number: 3962-66-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: SampIes for the concentration analysis by TOC were taken at 0, 24, 48 and 72 hours after start of exposure.
The calculated results derived from TOC analysis gave mean concentrations of 11.5 mg/L (saturated solution). - Vehicle:
- no
- Details on test solutions:
- For the preparation of the test solutions, a suspension with a nominal loading of 100 mg/L was ultrasonified (30 min) and stirred for approximately 24h at room temperature. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:1.25 by adding nutrient solution and inoculum for the highest concentration, and diluted 1:5, 1:10, 1:25, 1:50 and 1:100 with demineralized water, inoculum (2.5 mL) and nutrient solution (2.5 mL) for the other concentrations, all by electronically controlled syringe.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The alga was obtained from the "Sammlung von Algenkulturen, Universität Göttingen" and kept on slant agar containing a nutrient solution under standardized conditions (10-15 °C, diffuse light (light/dark rhythm 12h/12h)). This culture was dissolved in a nutrient solution and subsequently transferred once weekly.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- The temperature was 22-24 °C during the whole test period, fulfilling the validity criterion.
- pH:
- The pH was between 7.7 to 9.1. For technical reasons, no pH can be analysed at the beginning of the test. Since the pH cannot be adjusted throughout the test, this criterion has no further significance for the test. Therefore, the measurement of pH after incubation indicates whether extreme pH increases (>10) occurred due to algal growth.
- Nominal and measured concentrations:
- A nominal concentration of 100 mg/L was used as the stock solution. This corresponds to a measured concentration of 11.5 mg/L. It was further diluted 1:1.25 by adding nutrient solution and inoculum for the highest concentration, and diluted 1:5, 1:10, 1:25, 1:50 and 1:100 with demineralized water, inoculum (2.5 mL) and nutrient solution (2.5 mL) for the other concentrations, all by electronically controlled syringe.
- Details on test conditions:
- TEST SYSTEM
- Initial cells density: 100000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity and quality: 6000 - 8000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorescence every 24 h - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.14 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CI: 0.06 - 0.27 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.23 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 0.19 - 0.42 mg/L
- Details on results:
- As a parameter for the growth of the algal population, the fluorescence of the algal cells was measured with a fluorescence photometer. The increase in biomass and the growth rate was calculated on the basis of the fluorescence. The calculated biomass and growth rate of each concentration were compared to those of the controls, and the inhibition was calculated.
- Results with reference substance (positive control):
- No positive control used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus.
The EbC50 (biomass) was 0.14 mg/L [95% confidence limits: 0.06-0.27 mg/L], the ErC50 (growth rate) was 0.23 mg/L [95% confidence limits: 0.19-0.24 mg/L] on the basis of TOC- measured concentrations. - Executive summary:
The purpose of this study was to detennine the effects of the test compound Delta-5-Norandrostendion (ZK 67026) on the growth of the green algae Desmodesmus subspicatus. The study was conducted according to OECD 201.
The test substance was incubated in an aqueous solution including nutrients with an algae population of Desmodesmus subspicatus for a test duration of approximately 72 hours in an electronically controlled dosing and incubation apparatus. The nutrient solution was made up of mainly ammonia, phosphates and some trace elements. For the preparation of the test solutions a suspension with a nominal concentration of 100 mg/L in water was ultrasonified for approximately 30 min and stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution was used for the preparation of the test solutions. The highest concentration was obtained from a 1: 1.25 dilution by adding nutrient solution and inoculum, the further concentrations were obtained by dilutions of 1:5, 1:10, 1:25, 1:50, and 1:100 with demineralized water, nutrient solution and inoculum. Additionally, a control solution was prepared with demineralized water without test material. The organic carbon concentration of the stock solution was analyzed with a TOC analyzer in a sample taken at the start of the incubation. The substance concentration was calculated on the basis of the molecular formula. It was 11.5 mg/L. The further test concentrations were extrapolated from the result of the TOC-analysis and are shown in the table below. The algae were exposed to each concentration in triplicate. Six vessels were prepared for the control. The algae were incubated under standardized conditions (continuous light, controlled temperature). As a parameter for the growth of the algae population, the fluorescence of the algal cells was measured with a fluorescence-photometer. The increase of biomass and the growth rate were calculated on the basis of the fluorescence. The calculated biomass and growth rate of each concentration were compared to those of the controls, and the inhibition was calculated.
The biomass and growth rate was inhibited at all dilutions. This was considered to be a compound related effect. The study resulted in an ErC50 (72 h) of 0.23 mg/L (measured).
Reference
Table 1: Inhibition of biomass and the growth rate (%) of Desmodesmus subspicatus after 72 hours exposure to ZK 67026
Inhibition in percent ZK 67026 (mg/L) | |||
Dilution of ZK 67026 | Concentration of ZK 67026 | Biomass (integral) | Growth rate |
0 | 0.0 (control) | 0.0 | 0.0 |
1:100 | 0.1 | 43.0 | 16.3 |
1:50 | 0.2 | 74.0 | 50.7 |
1:25 | 0.5 | 87.3 | 82.0 |
1:10 | 1.2 | 93.6 | 107.5 |
1:5 | 2.3 | 91.6 | 97.6 |
1:1.25 | 9.2 | 100.1 | 112.3 |
Description of key information
The EbC50 (biomass) was 0.14 mg/L [95% confidence limits: 0.05-0.19 mg/L], the ErC50 (growth rate) was 0.23 mg/L [95% confidence limits: 0.20-0.27 mg/L] on the basis of TOC-measured concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.23 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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