Lithium difluoro(oxalato)borate(1-)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material:
Jiu Jiang Tinci Materials Technology Co., Ltd.
G200810043
- Purity, including information on contaminants, isomers, etc.:

>99.8%


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
In the refrigerator (2~8 °C), easy to hydrolyze and not in contact with moisture
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage:
Stable
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis:
Stable
- Solubility and stability of the test material in the solventehicle and the exposure medium:
The test substance is soluble in water.
- Reactivity of the test material with the incubation material used (e.g. plastic ware):
Non reactive

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Chemical Concentration in Stock Solution
Stock solution 1: Macro Nutrients
NH4Cl
1.5 g/L
MgCl2.6H2O 1.2 g/L
CaCl2.2H2O 1.8 g/L
MgSO4.7H2O 1.5 g/L
KH2PO4 0.16 g/L
Stock solution 2: Iron
FeCl3.6H2O
64 mg/L
Na2EDTA.2H2O 100 mg/L
Stock solution 3: Trace Elements
H3BO3
185 mg/L
MnCl2.4H2O 415 mg/L
ZnCl2 3 mg/L
CoCl2.6H2O 1.5 mg/L
CuCl2.2H2O 0.01 mg/L
Na2MoO4.2H2O 7 mg/L
Stock solution 4: Bicarbonate
NaHCO3 50 g/L

The pH of the test medium after equilibration in air should be approximately 8.1

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name:
Raphidocelis subcapitata (formerly Pseudokirchneriella subcapitata)
- Strain:
ATCC® 22662
- Source (laboratory, culture collection):
American Type Culture Collection
P.O. Box 1549
Manassas, VA 20108, USA
- Age of inoculum (at test initiation):
5 days
- Method of cultivation:
These conical flasks are incubated between 21 and 25 °C (± 2°C) under a continuous illumination of 8000 ± 1600 Lux.

ACCLIMATION
- Acclimation period:
5 days
- Culturing media and conditions (same as test or not):
same as test
- Any deformed or abnormal cells observed:
none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

22.2-22.5℃

pH:

7.64-7.87

Nominal and measured concentrations:

0, 0.4, 1.2, 3.6, 10.8, 32.4, 97.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
flask
- Material, size, headspace, fill volume:
conical flask, 200mL
- Aeration:
no
- Initial cells density:
10000 cells/ml
- Control end cells density:
707900 cells/ml
- No. of vessels per concentration (replicates):
6
- No. of vessels per control (replicates):
3


GROWTH MEDIUM
- Standard medium used:
yes
- Detailed composition if non-standard medium was used:



TEST CONCENTRATIONS
- Spacing factor for test concentrations:
3
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
0, 0.01, 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study:
%inhibition:0, 0, 0, 2.91, 39.07, 100

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

22.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

8.856 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

5.146 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

6.67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield (migrated information)

Key result

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

3.343 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield (migrated information)

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

2.231 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield (migrated information)

Results with reference substance (positive control):

- Results with reference substance valid?
YES
- EC50:
ErC50=0.6450 mg/L
EyC50=0.2130 mg/L
- Other:

Validity criteria fulfilled:

yes

Conclusions:

The EC50 and EC10 of toxicity to aquatic algae and cyanobacteria is 22.4 and 5.146 mg/L, respectively.

Description of key information

The EC50 and EC10 of toxicity to aquatic algae and cyanobacteria is 22.40 and 5.146 mg/L, respectively.

Key value for chemical safety assessment

EC50 for freshwater algae:

22.4 mg/L

EC10 or NOEC for freshwater algae:

5.146 mg/L