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EC number: 201-782-8 | CAS number: 87-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
Cyanuric acid is a potetial weak skin sensitiser in a LLNA study
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
- Species:
- mouse
- Strain:
- other: CBA/J
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Sprague-Dawley; Indianapolis, IN
- Age at study initiation: ~ 2 months
- Weight at study initiation: 21.6-26.8 g
- Housing: Housed individually in stainless steel cages, suspended with wire bottom
- Diet: PMI Feeds Inc. Formulab #5008; available ad libitum
- Water: Municipal water supply analyzed by TCEQ Water Utilities Division; available ad libitum
- Acclimation period: At least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C):19-21
- Humidity (%): 45-97
- Air changes (per hr): 10-12
- Photoperiod (hrs dark / hrs light): 12/12
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 100% undiluted; 50% and 25% w/v in 80% acetone; 20% olive oil
- No. of animals per dose:
- 5
- Details on study design:
-
MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: OECD 429
- Criteria used to consider a positive response: Stimulation index of ≥ 3.
TREATMENT PREPARATION AND ADMINISTRATION:
On days 1 ,2 and 3 each test animal received an open application of 25µL of an appropriate dilution (25% or 50%) of the test substance, or 100% test substance moistened with acetone:olive oil to the dorsum of both ears. On day 6 of the study all test and control animals were injected in the tail vein with 250µL of 0.01M phosphate-buffered saline (PBS), pH 7.4, containing 20 µCi of (methyl 11, 21 – 3H] Thymidine. Five hours after the injection, the animals were sacrificed, the draining auricular lymph nodes were excised and pairs from each animal were processed.
A single cell suspension was prepared by gentle mechanical disintegration through 200 mesh stainless steel gause. The cells were washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid at 4C for 18 hours. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- None applied
- Positive control results:
- The positive control elicited a stimulation index of 9.5.
- Parameter:
- SI
- Value:
- <= 3
- Test group / Remarks:
- 25%
- Remarks on result:
- other: Cyanuric acid produced a stimulation index of ≤ 3 in two of three groups of test animals. However, based on the severity of the response the positive results were considered indicators of borderline or mild skin sensitization potential.
- Parameter:
- SI
- Value:
- <= 3.4
- Test group / Remarks:
- 50%
- Remarks on result:
- not determinable because of methodological limitations
- Parameter:
- SI
- Value:
- <= 3.7
- Test group / Remarks:
- 100%
- Remarks on result:
- not determinable because of methodological limitations
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: Vehicle control: 668 Test group I (25%): 1390 Test group II (50%): 2244 Test group III (100%): 2500 Positive control: 6346
- Interpretation of results:
- other: mild sensitiser
- Conclusions:
- Cyanuric acid produced a stimulation index of ≥ 3 in two groups of animals. However based on the severity of the response the positive results were considered indicators of borderline or mild skin sensitisation.
- Endpoint:
- skin sensitisation: in vitro
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Referenceopen allclose all
Table 1:Ratio derived for each test group based on group mean DPM
Animal group |
Test substance concentration |
Average count per mouse |
Num. of mice in group |
Test/vehicle control ratio |
Vehicle control |
NA |
668 |
5 |
NA |
Test group I |
25% |
1390 |
5 |
2.1 |
Test group II |
50% |
2244 |
5 |
3.4 |
Test group III |
100% |
2500 |
5 |
3.7 |
Positive control |
NA |
6346 |
5 |
9.5* |
NA – not applicable
* - Positive control used to confirm animal sensitization potential and validate procedures
The test substance produced a stimulation index (SI) of ≤3 in the 25% dose group and ≥3 in the 50% and 100% dose groups, which is considered a positive sensitization response. However, based on the low magnitude of the SI compared to that of the positive control, which is a moderate sensitizer, cyanuric acid is considered to have weak or mild sensitization potential in this LLNA test. There were no signs of excessive irritation at any test site
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
In an OECD guideline study (Kuhn 2008) the sensitisation potential of cyanuric acid was investigated in female mice in a local lymph node assay.
The results of the LLNA study indicated that CYA elicited a positive response for potential skin sensitization, based on test/vehicle control ratio or stimulation index (SI) of 3 or greater in two of the three concentrations tested (50% and 100%). These positive results were considered indicators of borderline or mild skin sensitization potential. The test group at 25% CYA was below the SI of 3 threshold for a positive response. The severity of response was low, just above the SI threshold of 3 for only two of the three concentrations tested, which were considered borderline positive. In addition, the response at 25% CYA was below this threshold and not considered a positive response.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
The results of the LLNA study (Kuhn JO 2008) indicated that CYA elicited a positive response for potential skin sensitization, based on test/vehicle control ratio or stimulation index (SI) of 3 or greater in two of the three concentrations tested (50% and 100%). These positive results were considered indicators of borderline or mild skin sensitization potential. The test group at 25% CYA was below the SI of 3 threshold for a positive response. The severity of response was low, just above the SI threshold of 3 for only two of the three concentrations tested, which were considered borderline positive. In addition, the response at 25% CYA was below this threshold and not considered a positive response. On this basis CYA should not be classified as a skin sensitiser.
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