Cyclohexane-1,2,4-triyltris(ethylene)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Guideline 92/69/EEC

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: A saturated stock solution of 1g/L (nominal) has been tested by DOC analysis. The DOC was 1.98 mg/L and the stock soultion was diluted to the test concentratios 0.13 mg/L; 0.22 mg/L; 0.37 mg/L; 0.66 mg/L; 1.10 mg/L; and 1.98 mg/L.
- Sampling method: The DOC content of the stock solution has been meausured.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A suspension of 1 g/L of the test substance in purified water has been stirred for 18 hours. Then the solution was filtered and the carbon content was determined. The DOC content of the solution is 1.98 mg/L corresponding to 2.2 mg/L product. This solution was used as stock solution. The test concentrations were based on the product and calculated from the DOC of the stock solution. The test concentrations of 0.13 mg/L, 0.22 mg/L, 0.37 mg/L, 0.66 mg/L, 1.10 mg/L, and 1.98 mg/L were tested.
- Controls: test water without test substance
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not specified

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: Scenedesmus subspicatus (CHODAT 86.81 SAG)
- Source (laboratory, culture collection): Institute for water, soil, and air hygiene, Berlin, and breeding in the laboratory
- Age of inoculum (at test initiation): 3 days before test start a preculture was inoculated from a stock culture.

ACCLIMATION
- Acclimation period: 3 days before test start a preculture was inoculated from a stock culture.
- Culturing media and conditions (same as test or not): not specified

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

24 +/- 2 °C

pH:

at test start: 7.4 - 7.6
at test end: 8.5 - 8.6

Nominal and measured concentrations:

DOC of the stock solution 1.98 mg/L corresponding to 2.2 mg/L product concentration . Test concentration (calculated from stock solution) 0.13 mg/L; 0.22 mg/L; 0.37 mg/L; 0.66 mg/L; 1.10 mg/L; and 1.98 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, steril
- Aeration: yes
- Initial cells density: 20000 cells/mL
- Control end cells density: 81* 10^4 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8

GROWTH MEDIUM
- Standard medium used: not specified, according to guideline

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Light intensity and quality: approx. 8000 Lux, white light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of cell
- Determination of cell concentrations: photometric measurement. Absorption at 685 nmm, calculation of cells/mL by calibration curve

Reference substance (positive control):

no

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1.98 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: highest solubility under test conditions

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 1.98 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: highest solubility under test conditions

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 1.98 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: highest solubility under test conditions

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1.98 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.7 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.1 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

Based on the growth rate, an EC50 (0-72h) of >1.98 mg/L and an EC10 (0-72 h) of >1.98 mg/L were found. All concentration are based on the concentration of the test substance. Based on the biomass, an EbC50 of >1.98 mg/L and an EbC10 of 1.7 mg/L were determined. The NOEC based on biomass is 1.1 mg/L. The concentration of 1.98 mg/L is the highest solubilty under test conditions.

Reported statistics and error estimates:

It was not possible to calculate EC values according to probit transformation of the percental inhibition values with an regression analysis. The EC10 value has been determined graphically.

Validity criteria fulfilled:

yes

Remarks:

Valdity criteria are not specified in the report, but the criteria according to the guideline OECD202 (before 2006) are fulfilled. Also the validity criteria of the newer guideline (OECD 202, 2006/2011) would be met.

Conclusions:

Based on the growth rate, an EC50 (0-72h) of >1.98 mg/L and an EC10 (0-72 h) of >1.98 mg/L were found. The concentration of 1.98 mg/L corresponds to the highest solubilty under test conditions.

Description of key information

A toxicity test on Desmodesmus subspicatus with 1,2,4 -Trivinylcyclohexane revealed an ErC50 >1.98 mg/L and an ErC10 (0-72 h) of >1.98 mg/L based on the growth rate.

Additional information

A toxicity test on Desmodesmus subspicatus (previously Scenedesmus subspicatus) with 1,2,4 -Trivinylcyclyhexane is available and has been performed according to guideline 92/69/EEC and OECD201 (before 2006). The study has been conducted in accordance to GLP requirements and has acceptable restrictions (analytical method for measurement of the test substance concentration is not substance-specific), but is well documented.

Based on the growth rate, an ErC50 (0-72h) of >1.98 mg/L and an ErC10 (0-72 h) of >1.98 mg/L were found. The concentration of 1.98 mg/L corresponds to the highest solubilty under test conditions.