3-hydroxypropiononitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxypropiononitrile

EC Number:

203-704-8

EC Name:

3-hydroxypropiononitrile

Cas Number:

109-78-4

Molecular formula:

C3H5NO

IUPAC Name:

3-hydroxypropanenitrile

Details on test material:

- Name of test material (as cited in study report): Ethylencyanhydrin, 2-Cyanoethanol
- Analytical purity: 99.08%
- Stability under test conditions: no data
- Storage condition of test material: at 4°C in the refrigerator until use.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: <0,1 mbar (20°C)
- Water solubility (under test conditions): miscible

OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: inherently biodegradible
- Other: Hazard precautions: Avoid skin contact. Nonirritant.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Stock solution: A concentrated stock solution was prepared by mixing 10.0g of the test substance with 1000 mL of dilution water and stirring a
magnetic stirrer for 15 min; then it was put into the aquarium and the volume was adjusted with dilution water to 5L.
-Test solutions (prepared by dilution of the stock solution): 0 (blanc = control solution), 0.1, 1.0, 10, 100, 1000, 10000 mg/L concentration of the
test substance
- Procedure: Numbered 15-L-glass aquaria were used as test vessels and filled with 5L of test-solution. The test animals were added to the
previously prepared test solution. The aquaria were sligtly aerated via glass capillary throughout the test.
Test temperature: 23 ±1°C; pH(dilution water): 7.8 ±0.2

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish (Brachydanio rerio; short name: Danio rerio)
- Source: German bred
- Size: 2.0 ±1.0 cm
- Feeding during test: daily until 24 hours befor test
- Food type: Tetra Min(R)
- Amount: no data
- Frequency: no data

ACCLIMATION
- Acclimation period:48h
- Health during acclimation (any mortality observed): 2.5% mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

records at 24, 48, 72, 96 h

Test conditions

Test temperature:

23 °C ±1 °C

pH:

7.8 ±0.2

Dissolved oxygen:

11.7 mg/l O2

Nominal and measured concentrations:

Nominal: 0, 0.1, 1.0, 10, 100, 1000, 10000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 15-L-glass acquaria
- Number of test fish used per test concentration: 10
- Biomass loading rate: fish-weight less than 1,0 g fish/l of water
- Aeration: The acquaria were slightly aerated via glass capillara througout the test.
- Light/dark rythm: 14h (light)/10(dark)/day

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water was prepared according to test guideline No(1) following internation SOP`s.
- pH and oxygen concentrations of the control and of all the test mixtures were measured daily.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

- No mortality observed in the fish popuation at a test concentartion of 10000 mg/l

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

- No mortality observed in the fish popuation at a test concentartion of 10000 mg/l

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

- No mortality observed in the fish popuation at a test concentartion of 10000 mg/l

Details on results:

- Behavioural abnormalities: none
- Observations on body length and weight: none
- Mortality of control: 0%
- Other adverse effects control: none

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1: Cumulative Mortality of Zebra Fish (a)                                                                                      Cumulative percentage of dead fish  Nr   Test concentration (mg/l)  24h  48h  72h  96h  0/0  blanc  0  0   0   0   1 0.1   0  0   0   0   2 1.0  0  0   0   0   3 10.0  0   0   0   0   4 100.0  0   0   0   0   5 1000.0   0  0   0   0   6 10000.0  0  0  0   0   (a) In all test concentrations the fish were inconspicuous during the exposure time of 96h

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

- Criteria of OECD 203: Mortality of controls <1

Conclusions:

According to the presented results of the acute fish toxicity test: 96h LC50 (fish) >10 000 mg/l, the test substance Ethylencyanhydrin (2-Cyanoethanol) is classified as non toxic to fish (Brachydario rerio).

Executive summary:

According to the presented results of the acute fish toxicity test: 96h LC50 (fish) >10 000 mg/l, the test substance Ethylencyanhydrin (2-Cyanoethanol) is classified as non toxic to fish (Brachydario rerio).