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EC number: 820-015-3 | CAS number: 129874-15-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Range finder study only, but done to good standards and GLP
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- Cuprate(4-), [μ-[4-hydroxy-7-[4-[(2-hydroxyethyl)amino]-6-[[5-hydroxy-6-[(2-hydroxy-4-sulfophenyl)azo]-7-sulfo-2-naphthalenyl]amino]-1,3,5-triazin-2-yl]-3-[(2-hydroxy-4-sulfophenyl)azo]-2-naphthalenesulfonato(8-)]]di-, tetrasodium
- EC Number:
- 277-520-1
- EC Name:
- Cuprate(4-), [μ-[4-hydroxy-7-[4-[(2-hydroxyethyl)amino]-6-[[5-hydroxy-6-[(2-hydroxy-4-sulfophenyl)azo]-7-sulfo-2-naphthalenyl]amino]-1,3,5-triazin-2-yl]-3-[(2-hydroxy-4-sulfophenyl)azo]-2-naphthalenesulfonato(8-)]]di-, tetrasodium
- Cas Number:
- 73513-82-3
- Molecular formula:
- C37H21Cu2N9O17S4.4Na
- IUPAC Name:
- Cuprate(4-), [μ-[4-hydroxy-7-[4-[(2-hydroxyethyl)amino]-6-[[5-hydroxy-6-[(2-hydroxy-4-sulfophenyl)azo]-7-sulfo-2-naphthalenyl]amino]-1,3,5-triazin-2-yl]-3-[(2-hydroxy-4-sulfophenyl)azo]-2-naphthalenesulfonato(8-)]]di-, tetrasodium
- Test material form:
- solid: particulate/powder
- Details on test material:
- Trade name 2076 BENZANIL RUBINE 2BL
Purity: 55-60%
Constituent 1
- Specific details on test material used for the study:
- The substance tested was reported as the trade name Benzanil Rubine 2BL which is Direct Red 83-1. Purity was cited at 55-60% (impurities sodium chloride and water). Although dose levels do not appear to have been adjusted for purity, the absence of findings at 2000 mg/kg means that even if adjusting for purity, the disciminating dose is > 1000 mg/kg and classification is not needed. No further animal testing is justified.
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- Range-finding study
Dose level: 2000 mg/kg bw, one male and one female
Main study
Dose level: 2000 mg/kg bw, 5 males and 5 females - Control animals:
- no
- Details on study design:
- Procedure
All animals were dosed once only by gavage using a metal cannula attached to a graduated syringe. The volume administered to each animal was calculated according to its fasted bodyweight at the time of dosing.
Range-finding study
The animals were observed for deaths or overt signs of toxicity, ½, 1, 2 and 4 hr after dosing and subsequently once daily for 5 days.
Main study
The animals were observed for deaths or overt signs of toxicity, ½, 1, 2 and 4 hr after dosing and subsequently once daily for 14 days.
Individual bodyweights were recorded prior to dosing on Day 0 and Days 7 and 14.
At the end of the study the animals were killed by cervical dislocation and subjected to gross pathological examination. This consisted of an external examination and opening of the abdominal and thoracic cavities for examination of major organs. The appearance of any macroscopic abnormalities was recorded. No tissues were retained.
Results and discussion
- Preliminary study:
- Range-finding study
There were no deaths or clinical signs of toxicity.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- There were no deaths after 14 days.
- Clinical signs:
- Noisy respiration was noted in one female up to two hours after treatment. Red stained faeces was noted in one female four hours after treatment and in all animal at the Day 1 observation. Red stained fur was commonly noted in the females at the 4 hours and Day 1 observations and persisted in one female up to the Day 4 observation.
No other clinical signs of toxicity were noted. - Body weight:
- All animals showed expected gain in bodyweight during the study.
- Gross pathology:
- No abnormalities were noted at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute oral median lethal dose (LD50) of the test material, 2076 BENZANIL RUBINE 2BL, in the Sprague-Dawley CD strain rat was found to be greater than 2000 mg/kg bw.
- Executive summary:
The substance tested was reported as the trade name Benzanil Rubine 2BL which is Direct Red 83-1. Purity was cited at 55-60% (impurities sodium chloride and water). Although dose levels do not appear to have been adjusted for purity, the absence of findings at 2000 mg/kg means that even if adjusting for purity, the disciminating dose is > 1000 mg/kg and classification is not needed. No further animal testing is justified.
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