N-(2-chloroethyl)-N-ethylaniline

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-01-27 - 1992-01-29

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

1986; corresponds almost entirely OECD 209

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-chloroethyl)-N-ethylaniline

EC Number:

245-082-0

EC Name:

N-(2-chloroethyl)-N-ethylaniline

Cas Number:

22564-43-8

Molecular formula:

C11H16ClN

IUPAC Name:

N-(2-chloroethyl)-N-ethylaniline

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Weighing-in directly

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

- Pretreatment: none
- Initial biomass concentration: 6 g/L TS
- Source: laboratory sewage (OECD)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Test conditions

Nominal and measured concentrations:

100, 180, 320, 560 and 1000 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Results and discussion

Effect concentrations

Key result

Dose descriptor:

EC50

Effect conc.:

194 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no information on duration available

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The study was conducted under GLP according to ISO 8192:1986 (corresponds to OECD 209) on the registered substance itself. The method is to be considered scientifically reasonable with minor deficiencies in documentation, but the available information allows the conclusion that the test was properly conducted. Hence, the results can be considered as reliable to assess the effects of the test substance to aquatic organisms, here, activated sludge.
EC50 = 194 mg/L

Executive summary:

In a study conducted under GLP according to ISO 8192:1986 (corresponds to OECD 209), 100, 180, 320, 560 and 10000 mg/L of N-(2-chloroethyl)-N-ethyl-3-methylaniline were added to activated sludge and the respiration rate was determined. 3,5-Dichlorphenol served as positive control. The EC50 was determined to be 194 mg/L.