3-(2-ethylhexyloxy)propylamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 "Testverfahren mit Wasserorganismen (Gruppe L)."

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-(2-ethylhexyloxy)propylamine

EC Number:

226-420-6

EC Name:

3-(2-ethylhexyloxy)propylamine

Cas Number:

5397-31-9

Molecular formula:

C11H25NO

IUPAC Name:

3-[(2-ethylhexyl)oxy]propan-1-amine

Details on test material:

- Solubility in water: 15 g/L
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION
- The product was added to the test water without any pretreatment

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Animal species: Golden orfe
- Supplier: Fischzucht Paul Eggers, Hohenwestedt
- Mean body length of fish: 7.1 cm
- Mean body weight of fish: 4.6 g

HOUSING
- The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air
- Housing water properties:
- Total hardness: about 2.5 mmol/L
- Acid capacity: about 5.5 mmol/L
- Oxygen content: > 60% of max. saturation
- pH: about 8.0
- Photoperiod: 16 h light, 8 h darkness
- Water temperature: 19 - 21 °C
- Duration of housing and adaptation: about 3 months
- Mortality during the last 2 weeks of housing: about 2.5%
- Medical treatment: four times with 0.05 mg/L Malachite green chloride, twice with 10 mg/L Tetracycline hydrochloride
- Diet: growing feed F/B 50, ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20°C

pH:

7.5 - 9.1

Dissolved oxygen:

7.0 - 9.0 mg/L

Nominal and measured concentrations:

- Concentrations tested (nominal): 2.15, 4.64, 10, 21.5 and 46.4 mg/L
- Additionally, to study the effect of the high pH on the toxicity the concentrations of 21.5 and 46.4 mg/L were tested in parallel after pH-adjustment

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass aquarium (30 cm x 22 cm x 24 cm)
- Test volume: 10 L
- No. of animals/concentration: 10
- Loading (g fish/L test water): 2.8
- Adaptation of fish to test water and temperature: 3 days
- Withdrawl of food: 2 days

TEST WATER
- Test water: reconstituted freshwater according to DIN 38412, part 11 prepared from fully demineralized tap water
- Test water properties:
- Total hardness: 2.5 mmol/L
- Acid capacity: 0.8 mmol/L
- Ratio Ca/Mg ions: 4:1
- Ratio Na/K ions: 10:1
- pH: about 7.7
- Aeration: continuously with oil-free air

FURTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h darkness

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 4.64 - < 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Biological observations: test fish in the test concentration of 10 mg/L were tumbling

Results with reference substance (positive control):

LC50 (48 h) about 32 mg/L