methyl (E)-3-methyl-4-oxo-but-2-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, no deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

EC Number:

609-870-1

Cas Number:

40835-18-5

Molecular formula:

C6 H8 O3

IUPAC Name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 3-Formylcrotonsäuremethylester
- Physical state: Liquid
- Analytical purity: 99.2 %
- Impurities (identity and concentrations): not specified
- Lot/batch No.: Abl. Nr. 00-0007
- Storage condition of test material: ambient
- Water solubility: no details provided

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

the following concentrations were analysed at test start (0 h) and test end (48 h): 0, 6.25, 25 and 100 mg/L.

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4 medium for about 10 minutes at 20 ± 2°C
The nominal concentration of the stock solution was 100 mg/I .
By diluting this stock solution with M4 medium the following nominal concentrations (mg/1) were prepared : 6.25, 12.5, 25, 50, 100 mg/L
Control: M4-medium only

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain:
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche
Chimique Appliquée, France, in 1978 . From this date on this clone was cultured and bred continuously in the Laboratory of Experimental Toxicology and Ecology of BASF AF at Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Feeding during test: none


ACCLIMATION
- Acclimation period: none
- Type and amount of food: not indicated, however, asumed to be according to the test guideline
- Feeding frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.4 - 20.7

pH:

7.8 - 8.3

Dissolved oxygen:

8.4 - 8.7

Nominal and measured concentrations:

Nominal: 0, 6.25, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day night regime
- Light intensity: about 2 - 7 µE/m2*s at 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 0, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

4.12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

16.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

For the statistical calculation moving average method was used .