methyl (E)-3-methyl-4-oxo-but-2-enoate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, well documented, no deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

EC Number:

609-870-1

Cas Number:

40835-18-5

Molecular formula:

C6 H8 O3

IUPAC Name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

Test material form:

other: liquid

Details on test material:

IUCLID4 Test substance: as prescribed by 1.1 - 1.4
- Name of test material (as cited in study report):3-Formylcrotonsäuremethylester
- Physical state: Liquid
- Analytical purity: 99.2 %
- Impurities (identity and concentrations): not specified
- Purity test date:
- Lot/batch No.: Abl. Nr. 00-0007
- Storage condition of test material: ambient
- Water solubility: 654 mg/L

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Preparation of the inoculum :
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for 24 hours .
50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test .

No stock solution for the test substance was prepared, since the test substance was added in appropriate amounts to each of the proposed test concentrations.
For the reference substance, a stock solution of 500 mg/L was prepared.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Inoculum :
Activated sludge from laboratory wastewater plants treating municipal sewage . Concentration of dry substance
1 g/I .

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6,6 - 7,3

Nominal and measured concentrations:

Nominal: 0, 20, 100, 250, 500, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration inhibition

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2/5

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 85 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

ca. 350 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate