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Diss Factsheets
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EC number: 473-780-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2 November 2006 to 16 March 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP compliant; no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Two samples of the solvent control and each replicate test vessel were taken at 0 hours. One sample was analysed untreated and one sample after centrifugation (40000 g for approximately 30 minutes). Further samples (in duplicate) were taken at 0 hours and stored at approximately -20ºC for further analysis if necessary. In order to ensure sufficient sample volume for analysis at 72 hours two additional test replicates were prepared at 0 hours and incubated alongside the test. At 72 hours two samples of the solvent control and the test group were taken for analysis. One sample was analysed untreated and one sample after centrifugation (40000 g for approximately 30 minutes).
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- During the definitive test samples of the test preparations were analysed untreated and following centrifugation at 40000 g for 30 minutes. The
untreated samples indicated the total amount of test material present in the test preparations whilst the centrifuged samples indicated the amount of soluble and hence bioavailable test material present.
Analysis of the untreated test preparations at 0 hours showed measured test concentrations to range from 100% to 119% of nominal thereby indicating that the test system was correctly dosed. Analysis of the centrifuged test preparations showed measured test concentrations to range from 63% to 74% of nominal.
Analysis of both the untreated and centrifuged test preparations at 72 hours showed a decline in measured test concentrations in the range of 67% to 76% of nominal for the untreated test samples and 37% to 39% of nominal for the centrifuged test samples.
Given that the preliminary stability analyses conducted indicated that the test material was stable in culture medium over the test period this decline in measured concentrations was considered to be due to possible adsorption of the test material to the algal cells present. Whilst the preliminary recovery analyses conducted in the presence of algal cells indicated that no immediate adsorption occurred this does not preclude long-term adsorption over the test period. Adsorption was not a factor in the preliminary stability analyses since no algal cells were present.
Given this decline in measured test concentrations it was considered justifiable to base the results on the geometric mean measured test concentrations of the centrifuged test media in order to give a "worst case" analysis of the data.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Liquid cultures of Desmodesmus subspicatus were obtained from the Culture Collection of Algae and Protozoa (CCAP), Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland. Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. The master cultures were maintained in the laboratory under constant aeration and constant illumination at 21 ± 1°C.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 24 ± 1ºC, recorded daily
- pH:
- Determined at start and at 72h: 7.6-7.8
- Dissolved oxygen:
- Not determined
- Nominal and measured concentrations:
- Nominal: 0.0015 mg/l
Mean measured: 0.00076 mg/l
See also 'Details on test solutions' - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: covered
- Material, size, headspace, fill volume: 250 ml glass conical flask plugged with polyurethane foam bungs
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 3370 cells/ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- ASTM
OTHER TEST CONDITIONS
Maintained under constant illumination at approximately 7000 lux, 24 ± 1°C, constant aeration, constant shaken - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.002 mg/L
- Nominal / measured:
- nominal
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Sensitivity to control substance
ErC50 (0-72 h) : 0.58 mg/l
EbC50 (0-72 h) : 0.20 mg/l
EyC50 (0-72 h): 0.16 mg/l
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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