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Diss Factsheets
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EC number: 287-828-8 | CAS number: 85586-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, rape-oil, Me esters
- EC Number:
- 287-828-8
- EC Name:
- Fatty acids, rape-oil, Me esters
- Cas Number:
- 85586-25-0
- Molecular formula:
- Unspecified
- IUPAC Name:
- methyl (11Z)-icos-11-enoate; methyl (13Z)-docos-13-enoate; methyl (9E)-octadec-9-enoate; methyl (9Z,12Z)-octadeca-9,12-dienoate
- Details on test material:
- - Name of test material (as cited in study report): Rapeseed oil, fatty acid ester
- Physical state: yellowish liquid
- Analytical purity: 100%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Since the test substance is poorly soluble in water WAFs were prepared.
Test organisms
- Test organisms (species):
- Skeletonema costatum
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 19.1 - 21.9
- pH:
- 7.91 - 8.26
- Salinity:
- according to guideline
- Nominal and measured concentrations:
- Nominal: 3.2, 10, 18, 32 and 100 mg/L loading rate WAFs
- Details on test conditions:
- TEST SYSTEM
- Initial cells density: 10065 cells/mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous light
- Light intensity and quality: 7390 - 8190 lux
EFFECT PARAMETERS MEASURED: Cell number was counted after 72h exposure time.
- Determination of cell concentrations: Haemocytometer, Sedgewick-Rafter cell
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 100 and 1000 mg/L loading rate WAFs
- Results used to determine the conditions for the definitive study: EC100 at 100 mg/L loading rate WAF - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 33.92 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The marine algae Skeletonema costatum showed a 72h-EC50 value of 33.92 mg/L loading rate WAF in a toxicity test performed according to GLP and ISO guideline 10253.
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