3-chlorobutan-2-one

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Study performed before 2009.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorobutan-2-one

EC Number:

223-834-9

EC Name:

3-chlorobutan-2-one

Cas Number:

4091-39-8

Molecular formula:

C4H7ClO

IUPAC Name:

3-chlorobutan-2-one

Constituent 2

Reference substance name:

3-Chloro-2-butanone

IUPAC Name:

3-Chloro-2-butanone

Test material form:

other: 3% (v/v) aqueous solution

Details on test material:

no info about substance purity in study report

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

40 male Wistar rats bred at the institute were used for testing.
Bodyweight 225 +/- 28 g.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

other:

Vehicle:

air

Details on inhalation exposure:

Vapour generation: in a 1.5L glass container at 50°C
Volume inhalation chamber: 20 L
Air circulation: 516 +/- 16 L/h
Temperature: 22-24°C
Humidity: approx. 60% (air was dried with CaCl2)

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

ca. 8 h

Concentrations:

0.000; 0.021; 0.043; 0.144; 0.190; 0.408; 0.720; 1.010 mL/m3 air

No. of animals per sex per dose:

5 male rats per dose (7 dose groups and 1 controll group)

Control animals:

yes

Details on study design:

14 days observation period after 8 hours inhalative (vapour) administration

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 0.5 other: mL/m3 air

Based on:

test mat.

Mortality:

1.010 mg/m3 air: 2/5 during inhalation; 2/5 on day 2; 1/5 on day 3; overall mortality 5/5
0.720 mg/m3 air: 5/5 on day 2
0.408 mg/m3 air: 1/5 on day 10

Clinical signs:

other: Control group and two lowest dose groups: no clinical signs observed. All other dose groups: weight loss; increasing coarse and untended fur. Highest dose group: laboured breathing, uncoordinated movement, sedation.

Body weight:

Weight gain approx. 2 g per animal in the three lowest dose groups.
Weight loss 4 / 7 / 17 g in the 0.144; 0.190; 0.408 mL/m3 dose groups.

Gross pathology:

Highest dose group: lung oedema found in the animals that died during the inhalative administration.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Remarks:

Migrated information

Conclusions:

Classification as Toxicity Category IV.

Executive summary:

The LC50 for male Wistar rats after vapour inhalation over 8 hours was found to be approx. 0.5 mL/m3 air (14 days observation period).