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EC number: 284-660-7 | CAS number: 84961-70-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Remarks:
- Earthworm reproduction study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 20 - August 25, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- Qualifier:
- according to guideline
- Guideline:
- ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Appropriate amount of test substance was mixed into dry soil. Feed (cow manure) and water were then added.
- Controls: Negative control and positive control studies were also done. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: in-house
- Age at test initiation (mean and range, SD): 2-4 months
ACCLIMATION
- Acclimation period: 16 days
- Acclimation conditions (same as test or not): Yes, except that during acclimation the earthworms were kept in peat moss - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 56 d
- Test temperature:
- 20-22 degrees C
- pH:
- 7.44-7.59
- Moisture:
- 33.1-36.8%
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1L glass beakers covered with perforated plastic wrap secured with rubber bands.
- Amount of soil or substrate: 800 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 70% sand, 20% kaolin clay, 10% sphagnum peat, and 1% calcium chloride
- Maximum water holding capacity (in % dry weigth): 34%
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: 489-616 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Day 0: body weights; Day 28: physical abnormalities and body weight; Day 57: number of juveniles
VEHICLE CONTROL PERFORMED: no
- Nominal and measured concentrations:
- Nominal: 0. 62.5, 125, 250, 500, and 1000 mg/kg dry soil
- Reference substance (positive control):
- yes
- Remarks:
- Carbendazim
- Key result
- Duration:
- 28 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality at end of exposure period:
There was no mortality of adults in the negative control or test groups.
- Total mass of adults at beginning of test: mean 300.0 +/- 14.97 g at Day 0
- Changes in body weight of live adults (% of initial weight) at end of exposure period: +0.17-0.21 g, there was no significant difference in weight gain between the negative control and test groups.
- No. of offspring produced: Control 141, 62.5 mg/L 153, 125 mg/L 148, 250 mg/L 139, 500 mg/L 158, and 1000 mg/L 147.
- Other biological observations: Adults showed no abnormalities in appearance or behaviour. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 reproduction 0.8914 mg a.i./kg dry soil
- Other: NOEC 0.5 mg a.i./kg dry soil, LOEC 1 mg a.i./kg dry soil - Reported statistics and error estimates:
- See table below.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of Benzene, mono-C10-13-alkyl derivs., distn. residues for earthworms is >1000 mg/kg dry soil (nominal) based on adult mortality. The NOECs for growth and reproductive effects (based on number of offspring produced) were 1000 mg/kg dry soil. (nominal).
- Executive summary:
A study was performed to assess the toxicity of Benzene, mono-C10-13-alkyl derivs., distn. residues
to earthworms. Adult earthworms were exposed to concentrations of 0, 62.5, 125, 250, 500, and 1000 mg/kg dry soil of test substance for 28 days. The adults were then removed, and offspring were exposed for an additional 28 days. There were no behavioral or physical abnormalities noted. The EC50 for earthworms is >1000 mg/kg dry soil (nominal) based on adult mortality. The NOECs for growth and reproductive effects (based on number of offspring produced) were 1000 mg/kg dry soil. (nominal).
Reference
Concentration (mg/kg dry soil) |
Mortality (Percent) |
Mean Change in Body Weight (g) |
Mean Number of Juveniles |
0 |
0 |
+0.21 +/- 0.040 |
141 +/- 19.5 |
62.5 |
0 |
+0.18 +/- 0.029 |
153 +/- 10.4 |
125 |
0 |
+0.17 +/- 0.038 |
148 +/- 31.6 |
250 |
0 |
+0.20 +/- 0.013 |
139 +/- 32.0 |
500 |
0 |
+0.21 +/- 0.013 |
158 +/- 11.6 |
1000 |
0 |
+0.19 +/- 0.022 |
147 +/- 16.1 |
Description of key information
The EC50 of Benzene, mono-C10-13-alkyl derivs., distn. residues for earthworms is >1000 mg/kg dry soil (nominal) based on adult mortality. The NOECs for growth and reproductive effects (based on number of offspring produced) were 1000 mg/kg dry soil. (nominal).
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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