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EC number: 248-697-2 | CAS number: 27858-32-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 11, 1978 - May 19, 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Even though study pre-dates GLP and current guideline, the study method is comparable to OECD 471 (Bacterial reverse mutation assay) with one deviation of not using TA 102 bacterial strain.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Recommended TA102 bacterial strain was not used in the assay.
- Principles of method if other than guideline:
- Suspension of Salmonella Typhimurium cells were exposed to the test substance in the presence and in the absence of an exogenous metbolic activation system. These suspensions were mixed with with an overlay agar and plated immediately onto minimal medium. After two days of incubation, revertant colonies are counted and compared to the number of spontaneous revertant colonies on solvent control plates. Study was performed according to reference Ames, B. N., McCann, J. and Yamasaki, E. (1975) Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test. Mutat. Res. 31, 347-364.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(ethyl acetoacetato-O1',O3)bis(propan-2-olato)titanium
- EC Number:
- 248-697-2
- EC Name:
- Bis(ethyl acetoacetato-O1',O3)bis(propan-2-olato)titanium
- Cas Number:
- 27858-32-8
- Molecular formula:
- C18H32O8Ti
- IUPAC Name:
- Bis(ethylacetoacetato‐O1',O3")bis(propan‐2‐olato)titanium
- Details on test material:
- Name of test material (as cited in study report): Haskell No. 11,939-02; Titanic acid, Diacetoacetyl-diisopropyl; Tyzor DC
Constituent 1
Method
- Target gene:
- histidine locus in selected strains
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat-liver homogenate activation system (S-9)
- Test concentrations with justification for top dose:
- 1000, 3000, 5000, 7000 and 10000 µg test substance/plate with and without metabolic activation
- Vehicle / solvent:
- acetone
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- culture medium without solvent
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- used for assay with metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- test plate without glucose-6-phosphate and NAPDH
- Remarks:
- used for assay with metabolic activation
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- used for assay without metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- culture medium without solvent
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- used for assay without metabolic activation
- Positive controls:
- yes
- Positive control substance:
- other: N-Methyl-n'-nitro-N-nitrosoguanidine
- Remarks:
- used for assay without metabolic activation
- Details on test system and experimental conditions:
- Five histidine-requiring strains of Salmonella typhimurium were used in the mutagen assays.
Strains TA 1535 and TA 100 were used to detect base-pair substitution mutations, whereas strains TA 1537, T 1538 and TA 98 were used to detect frame-shift mutations. Two different experiments were performed to confirm the results.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Trial 1
Mutagenic activity of titanic acid, diacetoacetyl-diisopropyl with metabolic activation |
|||||
|
Histidine+Revertants per plate** |
||||
Test item / μg/plate |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
NSC |
22 |
5 |
32 |
40 |
168 |
Acetone |
20 |
13 |
32 |
39 |
187 |
Tyzor DC / 10000 * |
20 |
13 |
14 |
20 |
147 |
Tyzor DC / 1000 |
19 |
10 |
37 |
41 |
175 |
Tyzor DC / 3000 |
15 |
5 |
31 |
28 |
162 |
Tyzor DC / 5000 |
18 |
8 |
24 |
21 |
147 |
Tyzor DC / 7000 |
21 |
7 |
24 |
27 |
154 |
Tyzor DC / 10000 |
21 |
11 |
19 |
20 |
133 |
2AA / 5 |
- |
- |
- |
- |
1514 |
2AA / 10 |
555 |
1820 |
1889 |
- |
|
2AA/1 00 | - | 493 | - | - | - |
NSC - Nonsolvent control
Acetone – solvent control
TYZOR DC – titanic acid, diacetoacetyl-diisopropyl
2AA – 2-aminoanthracene (positive control)
* - Negative control plate without glucose-6 -phophate and NADP
** - Average number of revertants from 2 plates
Mutagenic activity of titanic acid, diacetoacetyl-diisopropyl without metabolic activation |
|||||
|
Histidine+Revertants per plate** |
||||
Test item / μg/plate |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
NSC |
35 |
8 |
14 |
24 |
159 |
Acetone |
28 |
5 |
25 |
26 |
159 |
Tyzor DC / 1000 |
34 |
7 |
20 |
23 |
140 |
Tyzor DC / 3000 |
22 |
8 |
17 |
25 |
116 |
Tyzor DC / 5000 |
20 |
6 |
16 |
24 |
119 |
Tyzor DC / 7000 |
22 |
4 |
13 |
20 |
104 |
Tyzor DC / 10000 |
15 |
4 |
14 |
19 |
103 |
MNNG / 2 |
1727 |
- |
- |
- |
2031 |
9AAc / 50 |
- |
386 |
- |
- |
- |
2NF / 25 | - | - | 1861 | 1958 | - |
NSC - Nonsolvent control
Acetone – solvent control
TYZOR DC –titanic acid, diacetoacetyl-diisopropyl
MNG – N-Methyl-N´-nitro-N-nitrosoguanidine (positive control)
9AAc - 9- Aminoacridine (positive control)
2NF - 2 -Nitrofluorene
** - Average number of revertants from 2 plates
Trial 2
Mutagenic activity of titanic acid, diacetoacetyl-diisopropyl with metabolic activation |
|||||
|
Histidine+Revertants per plate** |
||||
Test item / μg/plate |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
NSC |
22 |
15 |
32 |
47 |
180 |
Acetone |
28 |
10 |
36 |
40 |
198 |
Tyzor DC / 10000 * |
22 |
9 |
25 |
31 |
170 |
Tyzor DC / 1000 |
17 |
9 |
32 |
51 |
210 |
Tyzor DC / 3000 |
16 |
10 |
28 |
57 |
180 |
Tyzor DC / 5000 |
15 |
5 |
36 |
44 |
203 |
Tyzor DC / 7000 |
15 |
7 |
34 |
42 |
178 |
Tyzor DC / 10000 |
18 |
4 |
29 |
33 |
177 |
2AA / 5 |
- |
- |
- |
- |
1012 |
2AA / 10 |
473 |
1844 |
2102 |
- |
|
2AA/1 00 | - | 426 | - | - | - |
NSC - Nonsolvent control
Acetone – solvent control
TYZOR DC –titanic acid, diacetoacetyl-diisopropyl
2AA – 2-aminoanthracene (positive control)
* - Negative control plate without glucose-6 -phophate and NADP
** - Average number of revertants from 2 plates
Mutagenic activity of titanic acid, diacetoacetyl-diisopropyl without metabolic activation |
|||||
|
Histidine+Revertants per plate** |
||||
Test item / μg/plate |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
NSC |
23 |
10 |
12 |
34 |
162 |
Acetone |
24 |
8 |
11 |
15 |
147 |
Tyzor DC / 1000 |
25 |
8 |
12 |
12 |
133 |
Tyzor DC / 3000 |
20 |
8 |
12 |
12 |
133 |
Tyzor DC / 5000 |
17 |
4 |
8 |
13 |
16 |
Tyzor DC / 7000 |
12 |
10 |
9 |
14 |
96 |
Tyzor DC / 10000 |
10 |
8 |
8 |
13 |
81 |
MNNG / 2 |
1300 |
- |
- |
- |
1548 |
9AAc / 50 |
- |
534 |
- |
- |
- |
2NF / 25 | - | - | 1416 | 1949 | - |
NSC - Nonsolvent control
Acetone – solvent control
TYZOR DC –titanic acid, diacetoacetyl-diisopropyl
MNG – N-Methyl-N´-nitro-N-nitrosoguanidine (positive control)
9AAc - 9- Aminoacridine (positive control)
2NF - 2 -Nitrofluorene
** - Average number of revertants from 2 plates
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
negative with metabolic activation
Genetic toxicity in vitro of Bis(ethyl acetoacetato-O1',O3)bis(propan-2-olato)titanium was evaluated using five histidine-requiring strains of Salmonella Typhimurium. Test substance was not mutagenic in this bacterial reverse mutagenetic test. - Executive summary:
Bis(ethyl acetoacetato-O1',O3)bis(propan-2-olato)titanium was tested in Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 in concentrations up to 10 000 µg per plate. The compound was not mutagenic in the microbial assays either in the presence or absence of a liver microsomal system, i.e., it did not induce a significant increase over the spontaneous mutation frequency.
This study was regarded reliable with one restriction; the study does not include full range of recommended strains. However, the study performed is comparable to OECD 471 guideline. Thus, the result of this study is used as a key study in hazard assessment.
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