Pent-3-enenitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

from 05-NOV-2001 to 09-NOV-2001

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Based on its vapour pressure, pent-3-enenitrile is a volatile compound. As a result, this property must be taken into account in the experimental design of ecotoxicological studies. In the current data, no sufficient details are provided to be sure this precaution has been taken. Thus the results presented are believed not to be reliable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

There is no guideline indicated in the study report. Based on the few experimental details available, the method is closed to OECD Guideline 203. However, the level of information is too low to assess the potential deviations compared to this method.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pent-3-enenitrile

EC Number:

225-060-7

EC Name:

Pent-3-enenitrile

Cas Number:

4635-87-4

Molecular formula:

C5H7N

IUPAC Name:

(3E)-pent-3-enenitrile

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
No data

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

No data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

No data

Test temperature:

21.6 °C

pH:

Test initiation: 7.5 - 7.6
Test completion: 7.5

Dissolved oxygen:

Test initiation: 8.7 mg/L
Test completion: 6 - 6.8 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

0.1, 1, 10 and 100 mg/L (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
No further data

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Dissolved oxygen and pH were measured at the test initiation and completion (i.e. after 0 and 96 hours).
No further data

OTHER TEST CONDITIONS
No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality was assessed after 24, 48, 72 and 96 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
No further data

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

As there is no indication on the potential care given to the volatility of the test substance, it is not possible to exclude the existence of abiotic phenomenon of degradation susceptible to lead to the disappearance of the test substance from the medium. Therefore, no conclusion can be drawn out regarding the toxicity of the test substance to fish.

Executive summary:

The acute toxicity of the test substance to fathead minnow (Pimephales promelas) was assessed in a 96h-hour static test. Groups of 10 fish were exposed to a control treatment or to 3PN concentrations of 0.1, 1, 10 and 100 mg/L. Mortality was observed after 24, 48, 72 and 96 housr of exposure. At the end of the test period, no mortality was observed, neither in the control treatment, nor in groups exposed to the different concentrations of 3PN. However, as there is no indication on the potential care given to the volatility of the test substance, it is not possible to exclude the existence of abiotic phenomenon of degradation susceptible to lead to the disappearance of the test substance from the medium. Therefore, no conclusion can be drawn out regarding the toxicity of the test substance to fish.