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Diss Factsheets
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EC number: 200-939-8 | CAS number: 76-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect dated 1998.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect dated 2008.
- Qualifier:
- according to guideline
- Guideline:
- other: MAFF Japan Agricultural Chemicals Regulation Laws 2-1-3 Notification 12-Nousan-8147 and Notification 13 Seisan 1739 (2000 and 2001)
- GLP compliance:
- yes
- Test type:
- fixed concentration procedure
- Limit test:
- no
Test material
- Reference substance name:
- Perfluoroethane
- EC Number:
- 200-939-8
- EC Name:
- Perfluoroethane
- Cas Number:
- 76-16-4
- Molecular formula:
- C2F6
- IUPAC Name:
- hexafluoroethane
- Details on test material:
- - Purity: 99.99%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 8 weeks
- Weight at study initiation: 319.2-368.2 g (males); 213.0-232.9 g (females)
- Fasting period before study: no data
- Housing: individually in solid bottom caging with bedding and nestlets as enrichment
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26ºC (68-79ºF)
- Humidity (%): 30-70%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): approximate 12-hour light/dark cycle
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air and oxygen
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: cylindrical glass chamber with a glass baffle inside to promote uniform distribution of the test atmosphere
- Exposure chamber volume: 19 L
- Method of holding animals in test chamber: stainless steel, wire-mesh modules (sexes separate)
- Source and rate of air: houseline compressed air and oxygen; metered into 1-liter 3-neck mixing flasks by Brooks mass flow controllers (MFC); 10 L/minute
- Method of conditioning air: vapour and air mixture left the mixing flasks and entered a glass transfer tube where supplemental chamber oxygen was added to the mixture using a Brooks MFC
- Treatment of exhaust air: exhausted through a dry-ice cold trap and an MSA filter cartridge prior to discharge into the fume hood
- Temperature, humidity, pressure in air chamber: 20-24°C; 30-70%; no data
TEST ATMOSPHERE
- Brief description of analytical method used: gas chromatography 16 times during the 4-hour exposure; Chamber atmosphere was continually drawn from the exposure chamber and directly injected into a gas chromatograph (GC) equipped with a pneumatically operated gas sample valve and a flame ionization detector
- Samples taken from breathing zone: yes - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 500000 ± 9700 ppm
- No. of animals per sex per dose:
- 5 per sex
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: daily for first 7 days after exposure then once on Day 14
- Frequency of weighing: daily for first 7 days after exposure then once on Day 14
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 500 000 ppm
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- None
- Clinical signs:
- other: None
- Body weight:
- Three male rats displayed weight losses ranging from 3.4 to 12 grams on the day after the exposure. There were no other body weight losses in male rats throughout the recovery period. Four of 5 female rats displayed weight losses ranging from 0.1 to 19.4 grams on the day following the exposure, and one female rat continued to lose weight (3.8 grams) on post-exposure day 2. This same female rat gained 5.4 grams on post-exposure day 3 but then lost 8.7 grams on post-exposure day 4. There were no other body weight losses observed in female rats throughout the remainder of the recovery period.
- Gross pathology:
- Gross discoloration of the lungs was present in one male rat, was nonspecific, and is a common finding in rats of this strain and age. No other gross lesions were observed.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- LC50 > 500000 ppm.
The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability). - Executive summary:
One group of 5 male and 5 female Crl:CD(SD) rats was exposed whole-body to test substance vapours in air. The test atmosphere was generated by dilution of the test substance vapour in air and oxygen. Chamber concentrations of the test substance were measured by gas chromatography. Rats were weighed and observed for clinical signs of toxicity during a 14-day recovery period. After the recovery period, the rats were sacrificed and examined for gross pathologic abnormalities. All animals survived the exposure and subsequent 14-day recovery period.
Rats were exposed for 4 hours to a mean concentration of 500000 ± 9700 ppm (mean ± standard deviation). The rats’ startle responses were normal throughout the 4-hour exposure. There were no clinical signs of toxicity observed during the exposure or the recovery period. Three male rats displayed weight losses ranging from 3.4 to 12 grams on the day after the exposure. There were no other body weight losses in male rats throughout the recovery period. Four of 5 female rats displayed weight losses ranging from 0.1 to 19.4 grams on the day following the exposure, and one female rat continued to lose weight (3.8 grams) on post-exposure day 2. This same female rat gained 5.4 grams on post-exposure day 3 but then lost 8.7 grams on post-exposure day 4. There were no other body weight losses observed in female rats throughout the remainder of the recovery period.
Gross discoloration of the lungs was present in one male rat, was nonspecific, and is a common finding in rats of this strain and age. No other gross lesions were observed.
Under the conditions of this study, the 4-hour inhalation median lethal concentration (LC50) for the test substance in male and female rats was greater than 500000 ppm.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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