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EC number: 254-100-6 | CAS number: 38720-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 22 OCT 1979 to 25 OCT 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given: comparable to guideline
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: FDA guideline (Federal Register 38, No. 187, 27.9.1973)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 254-100-6
- EC Name:
- Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Cas Number:
- 38720-66-0
- Molecular formula:
- C20H10Cl2N2O2
- IUPAC Name:
- 1,8-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 1,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 2,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: in house breeding (Hoe:HIMK(SPFWiga))
- Weight at study initiation: 1.5 to 2 kg
- Housing: single caged
- Diet: standard diet ERKA 8300 (Futtermittelwerk Robert Koch oHG, Hamm/Westf., Germany), ad libitum
- Water: ad libitum
Test system
- Vehicle:
- other: polyethylene glycol 400
- Controls:
- not required
- Amount / concentration applied:
- 100 mg
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 mg test substance pasted with 3 drops of PEG 400 - Duration of treatment / exposure:
- 24 h
- Observation period (in vivo):
- 72 h
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): with physiological solution of sodium chloride
- Time after start of exposure: 24 h
SCORING SYSTEM: according to OECD TG 405
TOOL USED TO ASSESS SCORE: magnifier (at 48 and 72 h reading 1 drop of sodium-fluorescein (0.1%) added for scoring)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24-48-72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24-48-72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- , #2, #4
- Time point:
- other: 24-48-72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: redness
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 24-48-72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: redness
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #5
- Time point:
- other: 24-48-72 h
- Score:
- 0.67
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 72 h
- Remarks on result:
- other: redness
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #6
- Time point:
- other: 24-48-72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Remarks on result:
- other: redness
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- , #2, #4, #5, #6
- Time point:
- other: 24-48-72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- other: 24-48-72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritant / corrosive response data:
- Neither corneal nor iridial effects were observed at any time point during the test.
Conjunctival redness was observed in 6/6 animals at one and seven hours after instillation of the test item (score (redness): 1 or 2). This effect was fully reversible in 5/6 animals by 72 hours after application. In one animal this score ameliorated from 1 to 0 at the 48 hour reading, but then increased again to 1 at the 72 hour reading. Thus it cannot be stated as fully reversible in all animals.
Chemosis was visible in 2/6 animals at the one hour reading and in 1/6 animals at the 7 and 24 hour reading. This effect was fully reversible by 48 hours after application. - Other effects:
- There was discharge observed in 5/6 animals one hour after instillation of the test material as well as in 2/6 animals at the seven hour reading.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: other: CLP regulation
- Conclusions:
- It is concluded that the test is not irritating to eyes under these test conditions and has not to be classified according to the classification criteria of Regulation (EC) No 1272/2008. No corneal or iridial effects were observed. Conjunctival redness was observed in 6/6 animals at one and seven hours after instillation of the test item (score (redness): 1 or 2) as well as in 5/6 animals at the 24 h reading. These effects were fully reversible by 72 hours after application in 5/6 animals. In one animal this score ameliorated from 1 to 0 at the 48 hour reading, but then increased again to 1 at the 72 hour reading. Thus it cannot be stated as fully reversible in this animal. Nevertheless we assume this observation as not relevant due to the fact that the score has been already diminished to 0. Additionally in all other treated animals the observed positive conjunctival reactions vanished until the 72 hour reading. Chemosis was visible in 2/6 animals at the one hour reading and still in 1/6 animals at the 7 hour and 24 hour reading. This effect was fully reversible by 48 hours after application. Test material contains relevant amounts of the submission substance. Therefore the test results are considered adequate to fulfil the endpoint requirements.
- Executive summary:
Test material was subject to an acute eye irritation/corrosion test in 6 rabbits according to FDA guidelines (similar to OECD TG 405). No corneal or iridial effects were observed until the end of the observation period (72 h). Conjunctival redness was observed in 6/6 animals at one and seven hours after instillation of the test item (score (redness): 1 or 2) as well as in 5/6 animals at the 24 h reading. These effects were fully reversible by 72 hours after application in 5/6 animals. In one animal this score ameliorated from 1 to 0 at the 48 hour reading, but then increased again to 1 at the 72 hour reading. Chemosis was visible in 2/6 animals at the one hour reading and still in 1/6 animals at the 7 hour and 24 hour reading. This effect was fully reversible by 48 hours after application.
As it can be plausibly assumed that the minimal redness observed in 1/6 animals after 72 hours would have been reversible, if the observation time would have been longer and because the mean values of the observed eye responses were below the critical values for classification according to the criteria of Regulation (EC) No 1272/2008, the test material is judged not to be irritating to eyes and has not to be classified for eye irritation.
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