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EC number: 900-110-7 | CAS number: 5370-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14.9. - 04.10.1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: According to guideline under GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not applicable
- GLP compliance:
- yes
- Remarks:
- statement of compliance
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- C4-diacetale
- IUPAC Name:
- C4-diacetale
- Details on test material:
- - Name of test material (as cited in study report): c4-diacetale
- Analytical purity: 97.5%
- Storage condition of test material: RT
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 97, 98, 100, 102
- Details on mammalian cell type (if applicable):
- - Properly maintained: yes
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 33 - 5000 ug/plate
Results and discussion
Test results
- Species / strain:
- other: TA97, TA98, TA100, TA102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
C4-Diacetal (Ro 94-0101), precursor of beta carotene and astaxanthin synthesis,
shows borderline mutagenic activity in strain TA100. No mutagenic potential was
detected in the other three investigated Salmonella tester strains TA97, TA98
and TA102. - Executive summary:
C4-Diacetal (Ro 94-0101), a precursor of beta carotene and astaxanthin synthesis,
was evaluated for mutagenic activity in the Salmonella/mammalian microsome
assay (Ames test). Four strains (TA97, TA98, TA100 and TA102) were employed
with and without exogenous metabolic activation system (S9-mix). Positive
controls were performed in parallel to each experiment verifying the
sensitivity of the strains and the activity of the metabolic activation
system.
Ro 94-0101 was diluted in dimethylsulfoxide (DMSO) and tested up to 5'000
pg/plate, the recommended maximum test concentration for non toxic substances.
No precipitation of the compound after the addition to the aqueous medium was
observed. Toxic effects were apparent at 5'000 pg/plate without S9, except for
strain TA98, in the standard plate incorporation assay.
Ro 94-0101 induced a weak increase of the number of revertants at dose levels
around 1'000 to 5'000 pg/plate in strain TA100, whereas the other three
strains did not indicate a mutagenic potential of the test compound.
Thus it can be concluded, that Ro 94-0101 shows borderline mutagenic activity
in strain TA100 in the high dose range (> 1'000 ug/plate).
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