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Diss Factsheets
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EC number: 216-700-6 | CAS number: 1643-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01-03-1990 to 20-03-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- The quantitative reduction in the proliferation of bacteria caused by the test substance was assessed, measured as a comparison to the control in duplicate. The optical density was used.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material
No data
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 126 deg C
- Boiling point: n/a
- Vapour pressure: 1.7E-06 and 7.5E-05 Pa
- Water solubility: 409 g/L
- Henry's law constant: 3.7E-09-1.2E-08 Pa m³/mol
- log Pow: <2.7
- pKa: 4.01
- Stability in water: stable
- Stability in light: stable
- pH dependance on stability: n/a
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: readily biodegradable - Analytical monitoring:
- not specified
- Details on sampling:
- No data
- Vehicle:
- not specified
- Details on test solutions:
- No data
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture.
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 18 h
- Post exposure observation period:
- No data
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 50, 100, 600, 10000 and 100000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Type: Closed
- Material, size, headspace, fill volume: 80 mL
- Aeration: Sealed
- No. of vessels per concentration: Duplicate
- No. of vessels per control: Duplicate - Reference substance (positive control):
- no
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 80 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- No data
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC10 of the test item was determined to be 80 mg/L.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 25-03-1992 to 26-03-1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: German Standard Method DIN 38 412
- Deviations:
- not specified
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 126 deg C
- Boiling point: n/a
- Vapour pressure: 1.7E-06 and 7.5E-05 Pa
- Water solubility: 409 g/L
- Henry's law constant: 3.7E-09-1.2E-08 Pa m³/mol
- log Pow: <2.7
- pKa: 4.01
- Stability in water: stable
- Stability in light: stable
- pH dependance on stability: n/a
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: readily biodegradable - Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 16 h
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 188.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC100
- Effect conc.:
- 588 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 22.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Remarks:
- the test substance and the contol were in the prescribed ranges.
- Conclusions:
- The toxicity of the test substance was assessed in a cell multiplication inhibition test using Pseudomonas putida according to the German Standard Method DIN 38 412. The EC50 after 16 h was 188.7 mg/L.
Referenceopen allclose all
Bacterial toxicity of test item by the Bringmann-Kuhn method:
Concentration test item (mg/L) |
Cell proliferation Absorbance at 420 nm |
|
Test series 1 |
Test series 2 |
|
0 |
0.185 |
0.187 |
50 |
|
0.155 |
100 |
0.146 |
0.156 |
600 |
|
0.079 |
1000 |
0.056 |
0.056 |
10000 |
0.040 |
|
100000 |
0.020 |
|
Description of key information
No reliable studies are available for C12 AO. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C12 AO and the remainder comprises an analogue substance with very similar chain length.
Two reliable studies are available for C12 -14 AO. In the key study, Pseudomonas putida were exposed to C12 -14 AO for 18 hours in a Bringmann-Kuehn test. The EC10, based on reduction in growth rate, was 24 mg AO/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 24 mg/L
Additional information
No reliable studies are available for C12 AO. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C12 AO and the remainder comprises an analogue substance with very similar chain length.
Two reliable studies are available for C12 -14 AO. In the key study, Pseudomonas putida were exposed to nominal concentrations of 0, 50, 100, 600, 1000, 10000 or 100000 mg/L of C12 -14 AO for 18 hours in a Bringmann-Kuehn test [Kolvenbach M (1990)]. The EC10, based on reduction in growth rate, was 24 mg AO/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.