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Diss Factsheets
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EC number: 947-663-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant
- GLP compliance:
- not specified
- Remarks:
- Only signatures for GLP test plan available, GLP for test itself unclear
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.25, 12.5, 25, 50 and 100 mg/l (nominal)
- Sampling method: A two milliliter aliquot of each sample solution was withdrawn for analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: No solvent was required to prepare the test solutions
- Controls: Yes - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: Chesapeake cultures
- Age at study initiation (mean and range, SD): 28 - 31 days
- Length at study initiation (length definition, mean, range and SD): <=18 mm
- Feeding during test: No feeding during test
ACCLIMATION
- Type and amount of food: Laboratory prepared diet of commercial food (Zing/Tetramin)
- Feeding frequency: twice a day - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not relevant
- Hardness:
- 70 - 88 mg/l CaCO3
- Test temperature:
- 22 °C
- pH:
- 7.1 - 8.0
- Dissolved oxygen:
- 4.5 - 8.2
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25, 50 and 100 mg/l
Measured: 2.4, 4.9, 9.8, 16.3 and 41.3 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: vessel
- Material, size, headspace, fill volume: polypropylene, 2L filled with 1L test medium
- Aeration: Aerate by mixing test solutions to saturation prior to test; if dissolved oxygen falls below 40 percent saturation in any replicate during the test, supply oil free air at 100 ±10 bubbles per minute.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: US EPA moderately-hard reconstituted water:
NaHCO3 - 96 mg/L
CaS04.2H20 - 60 mg/L
MgSO4 - 60 mg/L
KCl - 4 mg/L
- Alkalinity: 72 - 100 mg/L CaCO3
- Conductivity: 232 - 248 umhos
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, pH, oxygen and conductivity were recorded daily
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: 50 to 100 ft candles
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, reflex loss, erratic swim are recorded daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- Probit Analysis or Spearman - Karber Method when possible, otherwise, graphical interpolation
- Sublethal observations / clinical signs:
For detailed results, see attached file "Results.doc".
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in controls at 0%, oygen saturation above 60%
- Conclusions:
- The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.
- Executive summary:
The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/l and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.
Reference
Description of key information
The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 15 mg/L
Additional information
The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/L and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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