N-phenyl-N'-[(phenylamino)sulfonyl]urea

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

9.7.-7.8.15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-phenyl-3-(phenylsulfamoyl)urea

EC Number:

813-358-5

Cas Number:

4886-26-4

Molecular formula:

C13H13N3O3S

IUPAC Name:

1-phenyl-3-(phenylsulfamoyl)urea

Test material form:

solid: particulate/powder

Details on test material:

- State of aggregation:
- Particle size distribution:
- Mass median aerodynamic diameter (MMAD):
- Geometric standard deviation (GSD):
- Shape of particles:
- Surface area of particles:
- Crystal structure:
- Coating:
- Surface properties:
- Density:
- Moisture content:
- Residual solvent:
- Activation:
- Stabilisation:
- Other:

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
- Expiration date of the lot/batch:
- Purity test date:

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)
Due to the insolubility of the test item in water, the test item was added directly into the test vessels. Carrier material was inert PE film. 56.0 – 56.1 mg of the test item was weighed and added into the test vessels.

OTHER SPECIFICS:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal WWTP Breisgauer Bucht (sampling date 7.7.15)
- Laboratory culture:
- Method of cultivation:
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge: 30 mg dra solids/L
- Initial cell/biomass concentration:
- Water filtered: yes/no
- Type and size of filter used, if any:

Duration of test (contact time):

29 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: standard medium
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 20.7 - 21.5 °C
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration: 30 mg/L dry solids
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus: gas wash bottles (2000 mL)
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: CO2-purified air was used (NaOH scrubber)
- Method used to create anaerobic conditions: -
- Measuring equipment: TOC-5050A total carbon analyser
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: 0.2 M NaOH
- Other:

SAMPLING
- Sampling frequency: day 0, 4, 7, 11, 14, 21, 28
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Abiotic sterile control:
- Toxicity control: 1
- Other:

STATISTICAL METHODS:

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

0.5

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Executive summary:

There was no degradation of the test item within the test duration of 28 days. The criterion for ready biodegradation was not met (degradation 60 %, 10-d- window), therefore the test item is not readily biodegradable.