2-Propenamide, N-(2-hydroxyethyl)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 July 2006 to 29 July 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch number: 050804
Purity: >99%

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method:
- Sample storage conditions before analysis:The test concentration of HEAA was measured using an LC-MS method of analysis. Four mid-vessel samples (20 ml) of media, were taken from the control and test vessels at 0 and 72 hours (fresh media) and at 24 and 96 hours (expired media) for analysis. On each occasion, two samples from each batch were analysed immediately, the remaining samples were stored in a refrigerator in case further analysis was required.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: On each occasion, the test substance (3000 mg) was dissolved in diluent water in a volumetric flask (5 l). The contents of the flask were shaken before being poured into a test vessel (glass aquarium) containing diluent water (20 l). The volume in the test vessel was then adjusted to 30 litres.
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium: The range finding test employed nominal concentrations of 10 and 100 mg/l. The definitive test concentration (nominally 100 mg/l) was selected based on the results of the range finding test.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none, test substance highly soluble.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain:
- Source: The fish were supplied by a commercial fish farm in the UK.
- Age at study initiation (mean and range, SD):
- Length at study initiation: Mean fork length = 4.30 cm.
- Weight at study initiation: Mean net weight = 1.52g.
- Method of breeding: They were reared at a commercial fish farm from eggs, which hatched in March 2006.

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not):
- Type and amount of food :Commercial fish food (TROUW (UK) Ltd; Nutra Fry 02); 1% to 3% of the total wet-weight of fish in the holding tank
- Feeding frequency:Daily
- Health during acclimation (any mortality observed):

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

184 to 190 mg/l CaCO3

Test temperature:

14.5 to 15.3°C

pH:

7.84 to 8.34

Dissolved oxygen:

85 to 103% air saturation value

Nominal and measured concentrations:

Exposure concentration: nominal 100 mg/l, measured 98 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 30 L
- Aeration: Supplementary aceration was provided via narrow bore glass tubes.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): daily batch renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: <1 g bodyweight/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations of the fish were made after approximately 0.25, 2, 4, 24, 48, 72 and 96 hours of exposure.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 98 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No mortality or treatment-related effects on the fish were exhibited during the study.
- Other biological observations: The measurements of water quality (temperature, pH, concentrations of dissolved oxygen and total hardness) remained within acceptable limits throughout the study. The test medium was clear and colourless.

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 value for HEAA with rainbow trout could not be calculated but must be >98 mg/l.
The “no observed effect concentration” was 98 mg/l.

Executive summary:

The study was designed to assess the acute toxicity of HEAA to rainbow trout under semi-static exposure conditions. The study was conducted in accordance with OECD Guideline 203, under GLP.

The 96-hour LC50 value for HEAA with rainbow trout could not be calculated but must be >98 mg/l. The “no observed effect concentration” was 98 mg/l.

Description of key information

Observations of the fish were made after approximately 0.25, 2, 4, 24, 48, 72 and 96 hours of exposure.

No mortality or treatment-related effects on the fish were exhibited during the study.

Based on these findings the following values have been estimated:

96-hour LC50 value : >98 mg/l

“No observed effect concentration” : 98 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 98 mg/L

Additional information

The study was designed to assess the acute toxicity of HEAA to rainbow trout under semi-static exposure conditions. The study was conducted in accordance with OECD Guideline 203, under GLP.

The 96-hour LC50 value for HEAA with rainbow trout could not be calculated but must be >98 mg/l. The “no observed effect concentration” was 98 mg/l.