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EC number: 500-457-0 | CAS number: 160901-19-9 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Nov 2020 - 01 Feb 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 2019
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sampling method: samples were taken at test start, after 24 h fresh and aged and after 48 and 72 h from fresh solutions. For nominal test item concentration 10.0 and 5.0 mg/L analytical samples from t = 0 h fresh and t = 24 h aged were analysed as at these test concentrations all fish were dead after 4-6 h.
- Sample storage conditions before analysis:The samples were mixed with 10 mL acetonitrile and stored deep-frozen (≤ - 18 °C) until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding the necessary amount of test item to a volumetric flask. Test medium was added and the solution was homogenised by shaking. The solution was observed to be turbid. Test item concentrations were prepared by dilution with test medium. The two highest test concentrations were observed to be turbid, all other solutions were observed to be clear and transparent. - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: bred at testing facility
- Weight at study initiation (mean and range, SD): average: 0.7440 g (10 fish)
ACCLIMATION
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: commercial fish food (inicio plus, BioMar SAS)
- Feeding frequency during acclimation: once per day, last feeding was performed two days prior exposure start
- Health during acclimation (any mortality observed): no
FEEDING DURING TEST (as applicable)
none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 196 mg CaCO3/L
- Test temperature:
- 21.9 – 22.9 °C
- pH:
- 7.50 – 7.95
- Dissolved oxygen:
- ≥ 90 % of air saturation
- Conductivity:
- 420 µS/cm
- Nominal and measured concentrations:
- Nominal test item concentrations: 10.0, 5.00, 2.50, 1.25, 0.625 mg/L
Time weighted mean concentrations: 5.70, 2.57, 1.47, 0.646, 0.316 mg/L (based on 3 representative constituents) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria (18 L nominal volume)
- Type (delete if not applicable): covered with lids
- Fill volume: 15 L
- Aeration: none
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.0245 g fish/L (control)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking water and deionised water
- Total organic carbon: ≤ 2.0 mg/L (non-GLP)
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 hours from fresh test solutions and after 24, 48, 72 and 96 hours from fresh and aged test solutions. Oxygen saturation was monitored after 2 h, 4 h and 6 h.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h darkness
- Light intensity: 738 Lux (mean)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and toxicity symptoms after 0 h, 4 -6 h, 24 h, 48 h, 51 – 54 h, 72 h, 75 – 78 h and 96 h of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
- Range finding study
- Test concentrations: 0.1, 1.0, 10.0 mg/L
- Results used to determine the conditions for the definitive study: 100% mortality at 10 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.19 mg/L
- 95% CI:
- >= 0.886 - <= 1.45
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C12EO2, C12EO3,and C12EO6
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.646 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C12EO2, C12EO3,and C12EO6
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: No sublethal effects were observed in the control and at test item concentrations up to and including 1.25 mg/L (nominal). At 2.50 mg/L (nominal) four showed reduced activity after 4 - 6 h and two fish after 24 h and 51 - 54 h.
- Mortality of control: no
- Other adverse effects control: no
The test item solutions in the glass aquaria were clear and transparent. - Reported statistics and error estimates:
- The NOEC and LOEC were determined by using a multiple comparison method (step down Cochran-Armitage test procedure). The LC50-value after 4 - 6 h and 24 h were determined following the trimmed Spearman-Karber procedure and the LC50-value after 48 h, 72 h and 96 h were determined by Weibull analysis using linear maximum likelihood regression.
- Sublethal observations / clinical signs:
Table 1: Validity criteria for OECD 203 (2019)
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
No mortality observed
yes
The dissolved oxygen concentration must have been at least 60 per cent of the air
saturation value throughout the test
≥ 90%
yes
There must be evidence that the concentration of the substance being tested has been
satisfactorily maintained, and preferably it should be at least 80% of the
nominal concentration throughout the test. If the deviation from the nominal
concentration is greater than 20 per cent, results should be based on the measured
concentration.
analytical monitoring performed
yes
Table 2: % mortality of fish
Test item nominal conc.[mg/L]
Control
0.625
1.25
2.50
5.00
10.0
Time [h]
Mortality [%]
4 – 6
0
0
0
0
100
100
Day 1 (24)
0
0
0
43
100
100
Day 2 (48)
0
0
0
71
100
100
Day 2 (51 - 54)
0
0
0
71
100
100
Day 3 (72)
0
0
0
86
100
100
Day 3 (75 - 78)
0
0
0
86
100
100
Day 4 (96)
0
0
0
86
100
100
Seven fish were used for each replicate in the test
Table 3: Observation of clinical signs of fish during test
Test item nominal conc.[mg/L]
Control
0.625
1.25
Time [h]
0
°
*
#
+
0
°
*
#
+
0
°
*
#
+
4 – 6
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 1 (24)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 2 (48)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 2 (51 - 54)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 3 (72)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 3 (75 - 78)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Day 4 (96)
7
0
0
0
0
7
0
0
0
0
7
0
0
0
0
Test item nominal conc.[mg/L]
2.50
5.00
10.0
Time [h]
0
°
*
#
+
0
°
*
#
+
0
°
*
#
+
4 – 6
3
4
0
0
0
0
0
0
0
7
0
0
0
0
7
Day 1 (24)
2
2
0
0
3
0
0
0
0
7
0
0
0
0
7
Day 2 (48)
2
0
0
0
5
0
0
0
0
7
0
0
0
0
7
Day 2 (51 - 54)
0
2
0
0
5
0
0
0
0
7
0
0
0
0
7
Day 3 (72)
1
0
0
0
6
0
0
0
0
7
0
0
0
0
7
Day 3 (75 - 78)
1
0
0
0
6
0
0
0
0
7
0
0
0
0
7
Day 4 (96)
1
0
0
0
6
0
0
0
0
7
0
0
0
0
7
0 = no clinical signs
° = unusual behaviour (reduced activity and/or orientation to bottom or surface of the test vessels, dark pigmentation, dark eyes)
* = difficulties with maintenance of equilibrium
# = fish upside down with loss of equilibrium, showing only movement of gills as a sign of life
+ = no sign of life
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 12 SEP - 16 SEP 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source: Aqua Ivrea, taly
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.0 ± 1 cm
- Weight at study initiation (mean and range, SD): 0.35 ± 0.15 g
ACCLIMATION
- Acclimation period: >=7 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 23 - 24 °C
- pH:
- 7.11 - 7.69 (control)
7.06 - 7.69 (treatment) - Dissolved oxygen:
- 6.0 - 8.4 mg/L (control)
5.0 - 8.4 mg/L (treatment) - Nominal and measured concentrations:
- Nominal test substance concentrations: 1, 1.8, 3.2, 5.6 and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Intervals of water quality measurement: every 24 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms at 0, 24, 48, 72and 96 h
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.8 - 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations:
Decreased excape reflex was recorded at 24 h in all the fish treated with 1 and 1.8 mg/L. The fish recovered at the 48 h observation.
- Mortality of control: no
- Other adverse effects control: no - Sublethal observations / clinical signs:
Nominal concentration
[mg/L]Test period (h)
24 h
48 h
72 h
96 h
Mortality [%]
Mortality [%]
Mortality [%]
Mortality [%]
Control
0
0
0
0
1.0
0
0
0
0
1.8
0
0
0
0
3.2
100
100
100
100
5.6
100
100
100
100
10
100
100
100
100
Referenceopen allclose all
Description of key information
LC50 (96 h): 1.19 mg/L (TWA).
Key value for chemical safety assessment
Additional information
The acute toxicity of Alcohols, C12-13, branched and linear, ethoxylated to fish was investigated in a GLP guideline study following OECD 203. Seven fish of the species Pimephales promelas were exposed to nominal test item concentrations of 0.0625, 1.25, 2.50, 5.00 and 10.0 mg/L under semi-static conditions. Test solutions were renewed every 24 hours.
The test concentrations were analytically monitored by HPLC-MS/MS. The time weighted mean measured concentrations, based on three representative constituents of the UVCB substance, were 0.316, 0.646, 1.47, 2.57, 5.70 mg/L. No mortalities were observed up to and including a measured concentration of 0.646 mg/L. The determined LC50 (96 h) was 1.19 mg/L (TWA).
In a supporting study, performed according to OECD 203 with Danio rerio as test organism, a LC50 (96 h) between 1.8 – 3.2 mg/L (nominal) was recorded.
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