5-chloro-2-(2,4-dichlorophenoxy)aniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP screening test without concentration control analysis; however, the substance is considered as stable during the entired test period.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Remarks:

non-GLP screening test

Test material

Test material information

Constituent 1

Reference substance name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

EC Number:

260-480-4

EC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Cas Number:

56966-52-0

Molecular formula:

C12H8Cl3NO

IUPAC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Constituent 2

Reference substance name:

5-chloro-2-(2,4-dichloro-phenoxy)-aniline

IUPAC Name:

5-chloro-2-(2,4-dichloro-phenoxy)-aniline

Details on test material:

- Name of test material (as cited in study report): 5-chloro-2-(2,4-dichloro-phenoxy)-aniline
- Analytical purity: 99.8 area-%
- Lot/batch No.: BAFU-889795-6, Batches TADE 236 09-240 09
- Test substance No.: 09/0872-1
- CAS No.: 56966-52-0

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in OECD Medium for about 20 hours at approximately 20±2°C. The stock
solution was clear and colorless. The test concentrations were prepared by diluting the stock solution.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): The test strain is obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and is kept in liquid culture in the Laboratory of Experimental Toxicology and Ecology.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23+/- 2 °C

pH:

6 - 9

Nominal and measured concentrations:

Nominal: 0, 0.00001, 0.0001, 0.001, 0.01, 0.1, 1 mg/L based on test substance.

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Fill volume: 20 ml
- Initial cells density: 5000
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


GROWTH MEDIUM
- Standard medium used: yes (Medium according to OECD guidelines)


OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, about 60 - 120 microE/(m²*s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) after 0, 24, 48 and 72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.15 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.32 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

0.69 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate