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EC number: 231-831-9 | CAS number: 7758-05-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of various test chemicals
- Justification for type of information:
- Experimental data of various test chemicals is from collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- WoE report is based on three short term toxicity study of test chemical on aquatic algae and cyanobacteria.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- other: 1: Dunaliella salina, 2: Chlorella sp., 3: Cryptomonas sp.
- Details on test organisms:
- 1: Serial concentrations of test chemical were supplemented into the modified medium for the culture of Dunaliella salina.
2: Serial concentrations of test chemical were supplemented into the f/2 medium for Chlorella sp.
3: Serial concentrations of potassium iodide were supplemented into the f/2 medium for Cryptomonas sp. - Test type:
- static
- Water media type:
- other: 1: saltwater, 2: brackish water
- Total exposure duration:
- 10 d
- Test temperature:
- 25 deg C
- Dissolved oxygen:
- above 45% saturation.
- Details on test conditions:
- The inocula, each containing 3 × 10(4) alga cells, were added to 10mL of the corresponding media and incubated in an oscillator shaker at a rate of 150 rpm under light irradiation of 81.04μmolm−2 s−1 and a 12 h/12 h light/dark cycle.
- Reference substance (positive control):
- no
- Duration:
- 10 d
- Dose descriptor:
- other: MIC
- Effect conc.:
- 356.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1st study
- Duration:
- 10 d
- Dose descriptor:
- other: MIC
- Effect conc.:
- 696.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2nd study
- Duration:
- 10 d
- Dose descriptor:
- other: MIC
- Effect conc.:
- 475.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 3rd study
- Details on results:
- The growth of algae was determined by counting the number of cells using a haemacytometer. The minimum concentration of test chemical that inhibited growth was designated as MIC100.
- Validity criteria fulfilled:
- no
- Conclusions:
- 1: Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Dunaliella salina) in a 10 days. study on growth effect was determine to be 356.8 mg/L.Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that test chemical does not exhibit toxicity to green algae (Dunaliella salina).
2: Minimum Inhibitory Concentration (MIC100) of test chemical in green algae (Chlorella sp.) in a 10 days study on growth effect was observed to be 696.2 ± 9.4 mg/L.
3: Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Cryptomonas sp.) in a 10 days. study on growth effect was determine to be 475.9 ± 10.3 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Cryptomonas sp). - Executive summary:
Summarized result for the toxicity of test chemical on the growth of algae were studied and reviewed. The details are as mention below:
To evaluate toxicity of test chemical on the growth of Dunaliella salina. Saltwater Dunaliella salina were used as a test organism which exposed for the 10 days of incubation. Serial concentrations of test chemical were supplemented into the modified medium for the culture of Dunaliella salina. The inocula, each containing 3 × 10(4) alga cells, were added to 10mL of the corresponding media and incubated in an oscillator shaker at a rate of 150 rpm under light irradiation of 81.04μmolm−2 s−1 and a 12 h/12 h light/dark cycle. Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Dunaliella salina) in a 10 days. study on growth effect was determine to be 356.8 mg/L.Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that test chemical does not exhibit toxicity to green algae (Dunaliella salina).
First study was supported by the second study from peer reviewed journal. In this study the effects of chemical on the growth and metabolite accumulation of Chlorella sp. were investigated to assess its possible application to the mass culture of the two diatoms in open environment, extensive systems. Test conducted under the static system for 10 days. brackish water Chlorella sp. maintain under the proper conditions of temperature and pH. Serial concentrations of test chemical were supplemented into the f/2 medium for Chlorella sp. At concentrations >700 mg/l, the growth of Chlorella sp. was inhibited. Minimum Inhibitory Concentration (MIC100) of test chemical in green algae (Chlorella sp.) in a 10 days study on growth effect was observed to be 696.2 ± 9.4 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Chlorella sp.) and thus not classified.
Similarly in the third study aim of this study was to determine the effect of test chemical on the growth of Cryptomonas sp. Test conducted under the static system for the total exposure period of 10 days. Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Cryptomonas sp.) in a 10 days. study on growth effect was determine to be 475.9 ± 10.3 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Cryptomonas sp) and thus not classified as per the classification criteria.
Thus based on the above data from various peer reviewed journals, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
1: Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Dunaliella salina) in a 10 days. study on growth effect was determine to be 356.8 mg/L.Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that test chemical does not exhibit toxicity to green algae (Dunaliella salina).
2: Minimum Inhibitory Concentration (MIC100) of test chemical in green algae (Chlorella sp.) in a 10 days study on growth effect was observed to be 696.2 ± 9.4 mg/L.
3: Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Cryptomonas sp.) in a 10 days. study on growth effect was determine to be 475.9 ± 10.3 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Cryptomonas sp).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 356.8 mg/L
Additional information
Summarized result for the toxicity of test chemical and structurally and functionally similar read across chemicals on the growth of algae were studied and reviewed. The details are as mention below:
To evaluate toxicity of test chemical on the growth of Dunaliella salina. Saltwater Dunaliella salina were used as a test organism which exposed for the 10 days of incubation. Serial concentrations of test chemical were supplemented into the modified medium for the culture of Dunaliella salina. The inocula, each containing 3 × 10(4) alga cells, were added to 10mL of the corresponding media and incubated in an oscillator shaker at a rate of 150 rpm under light irradiation of 81.04μmolm−2 s−1 and a 12 h/12 h light/dark cycle. Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Dunaliella salina) in a 10 days. Study on growth effect was determine to be 356.8 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that test chemical does not exhibit toxicity to green algae (Dunaliella salina).
First study was supported by the second study from peer reviewed journal. In this study the effects of chemical on the growth and metabolite accumulation of Chlorella sp. were investigated to assess its possible application to the mass culture of the two diatoms in open environment, extensive systems. Test conducted under the static system for 10 days. Brackish water Chlorella sp. maintain under the proper conditions of temperature and pH. Serial concentrations of test chemical were supplemented into the f/2 medium for Chlorella sp. At concentrations >700 mg/l, the growth of Chlorella sp. was inhibited. Minimum Inhibitory Concentration (MIC100) of test chemical in green algae (Chlorella sp.) in a 10 days study on growth effect was observed to be 696.2 ± 9.4 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Chlorella sp.) and thus not classified.
Similarly in the third study aim of this study was to determine the effect of test chemical on the growth of Cryptomonas sp. Test conducted under the static system for the total exposure period of 10 days. Minimum Inhibitory Concentration (MIC100) value of test chemical in green algae (Cryptomonas sp.) in a 10 days study on growth effect was determine to be 475.9 ± 10.3 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit toxicity to green algae (Cryptomonas sp) and thus not classified as per the classification criteria.
Thus based on the above data from various peer reviewed journals, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
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