N,N-diethyl-3-oxobutyramide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of microorganism for the test chemical :1.Respiration inhibition test performed for the test material on activated sludge2.Cell multiplication inhibition test was performed on Pseudomonas putida

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC Name: N,N-diethyl-3-oxobutyramide- InChI: 1S/C8H15NO2/c1-4-9(5-2)8(11)6-7(3)10/h4-6H2,1-3H3- Smiles: N(C(CC(C)=O)=O)(CC)CC- Molecular formula :C8H15NO2- Molecular weight :157.212 g/mol- Substance type:Organic- Physical state:Pale yellow clear liquid

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: 1.Activated sludge 2.Pseudomonas putida

Details on inoculum:

1. activated sludge of a predominantly domestic sewage

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Test temperature:

1.23 degree C

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 600 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

other: TT

Effect conc.:

67 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical N,N-diethyl-3-oxobutyramide is not likely to be toxic to microorganism atleast in the concentration range of > 16000 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical N,N-diethyl-3-oxobutyramide (2235 -46 -3).The studies are as mentioned below:

1. Activated sludge respiration inhibition test was performed for the test material .activated sludge of a predominantly domestic sewage was subjected to the test for 30 min .15% increase in oxygen consumption was observed , No inhibition was observed . The effect concentration EC50 of test material after 30 min of inhibition test was observed to be >16000 mg/l.

2.In the cell multiplication inhibition test the effect of test material on microorganisms was studied for Pseudomonas putida. The toxicity threshold (TT = EC3) after a 16 h exposure period has been determined to be 67mg/l indicating only slight to moderate toxicity in this specific bacterial strain.

 

The test chemical is readily biodegradable , therefore it can be considered to be not toxic to aquatic environment.

 

Description of key information

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical N,N-diethyl-3-oxobutyramide (2235 -46 -3).The studies are as mentioned below:

1. Activated sludge respiration inhibition test was performed for the test material .activated sludge of a predominantly domestic sewage was subjected to the test for 30 min .15% increase in oxygen consumption was observed , No inhibition was observed .The effect concentration EC50 of test material after 30 min of inhibition test was observed to be >16000 mg/l.

2.In the cell multiplication inhibition test the effect of test material on microorganisms was studied for Pseudomonas putida. The toxicity threshold (TT = EC3) after a 16 h exposure period has been determined to be 67mg/l indicating only slight to moderate toxicity in this specific bacterial strain.

 

The test chemical is readily biodegradable , therefore it can be considered to be not toxic to aquatic environment.

 

Key value for chemical safety assessment

EC50 for microorganisms:

16 000 mg/L

Additional information

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical N,N-diethyl-3-oxobutyramide (2235 -46 -3).The studies are as mentioned below:

1. Activated sludge respiration inhibition test was performed for the test material .activated sludge of a predominantly domestic sewage was subjected to the test for 30 min .15% increase in oxygen consumption was observed , No inhibition was observed .The effect concentration EC50 of test material after 30 min of inhibition test was observed to be >16000 mg/l.

2.In the cell multiplication inhibition test the effect of test material on microorganisms was studied for Pseudomonas putida. The toxicity threshold (TT = EC3) after a 16 h exposure period has been determined to be 67mg/l indicating only slight to moderate toxicity in this specific bacterial strain.

 

The test chemical is readily biodegradable , therefore it can be considered to be not toxic to aquatic environment.