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EC number: 212-001-5 | CAS number: 736-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed GLP compliant study using a different set of tester strains as compared to actual guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Principles of method if other than guideline:
- a different set of tester strains was used as compared to actual guidelines
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,1'-ethane-1,2-diylbis(4-nitrobenzene)
- EC Number:
- 212-001-5
- Cas Number:
- 736-30-1
- Molecular formula:
- C14H12N2O4
- IUPAC Name:
- 1,1'-ethane-1,2-diylbis(4-nitrobenzene)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 mix
- Test concentrations with justification for top dose:
- 1.0, 10.0, 100.0, 500.0, 1000.0, 2500.0, 5000.0, 10000.0 µg per plate with and without metabolic activation
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-Anthramine
- Evaluation criteria:
- (1) Strains TA-1535, TA-1537 and TA-1538
If the solvent control value is within the normal range, a test
material producing a positive response equal to or above three times the solvent
control value is considered mutagenic.
(2) Strains TA-98 and TA-l00
If the solvent control value is within the normal range, a test
material producing a positive response equal to or above twice the solvent
control value is considered mutagenic. - Statistics:
- not required
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- in TA-1538, TA-98 and TA-l00 with and without metabolic activation
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
The test material exhibited genetic activity with the strains TA-1538, TA-98 and TA-100 with and without metabolic activation and was therefore considered to be mutagenic under these test conditions. - Executive summary:
The test compound was examined for mutagenic activity in a series of in vitro microbial assays employing Salmonella indicator organisms (TA 98, TA100, TA 1535, TA 1537, TA 1538). The compound was tested in the absence and in the presence of liver microsomal enzyme preparations from Aroclor-induced rats.
A negative control consisting of the solvent used for preparing the stock solutions and subsequent dilutions of the test material and specific positive compounds were also assayed concurrently with the test material. The negative control data was used as the basis for evaluating the results obtained with the test material.
Doses were selected from a preliminary study conducted with the test material at 14 doses of 1.22 µg to 10,000 µg per plate using the strain TA-100.
In the preliminary toxicity study, the test material did not exhibit toxicity to the indicator strain at any of the doses tested as evidenced by the presence of the background lawn on the minimal plates. As such, the mutagenicity assays were conducted at 8 doses of 1.0 µg to 10,000.0 µg per plate.
The test conducted on the test material in the absence of a metabolic activation system was positive with strains TA-1538, TA-98 and TA-100.
The test conducted on the test material in the presence of a rat liver activation system was positive with strains TA-1538, TA-98 and TA-100.
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