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Diss Factsheets
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EC number: 608-929-9 | CAS number: 33955-44-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, no deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione
- EC Number:
- 608-929-9
- Cas Number:
- 33955-44-1
- Molecular formula:
- C22H11NO2
- IUPAC Name:
- 1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): PERYLMONOIMID PULVER
- Molecular formula (if other than submission substance): C22 H11 N 02
- Molecular weight (if other than submission substance): 321.33
- Physical state: solid
- Analytical purity: 79.0 g/100 g by ŠH-NMR spectroscopy using the internal standard method
- Purity test date: 06.07.2000
- Lot/batch No.: Partie 6/99
- Stability under test conditions: given
- Special storage condition of test material: none
- Water solubility: insoluble
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Handling of a poorly water-soluble test substance:
The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added , the pH-value was adjusted to 7,5 ± 0,5, the inoculum was added and the test started.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- lnoculum:
Activated sludge from Iaboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/I.
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5.g/1 dry substance and aerated dur ing the
night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/1 dry substance in the
test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 +/- 2°C
- Dissolved oxygen:
- >= 2.5 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1 g/L dry substance
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC20: 1.6 mg/L
EC50: 5.8 mg/L
EC80: 38 mg/L - Reported statistics and error estimates:
- not applicable
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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