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EC number: 231-104-6 | CAS number: 7439-95-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of magnesium sulphate on adriamycin-induced calstogenic and biochemical changes in Swiss Albino Mice
- Author:
- Al-Shabanah OA
- Year:
- 1 998
- Bibliographic source:
- Chemotherapy 44: 272-283
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The micronucleus test procedure described by Schmid was used (Schmid W, 1975, The micronucleus test. Mutation
Research 31: 9-15.). - GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Magnesium sulphate
- IUPAC Name:
- Magnesium sulphate
- Reference substance name:
- Magnesium sulphate
- EC Number:
- 231-298-2
- EC Name:
- Magnesium sulphate
- Cas Number:
- 7487-88-9
- IUPAC Name:
- 7487-88-9
- Test material form:
- solid: compact
- Details on test material:
- - Molecular formation: MgSO4
- Supplier: BDH Limited, Poole, UK
- Lot No.: Not reported
- Purity: Not reported
- Storage condition: Not reported
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Experimental Animal Car Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia)
- Age at study initiation: 6 to 8 weeks
- Weight at study initiation: 25 to 30 grams
- Assigned to test groups randomly: yes
- Fasting period before study: Not reported
- Housing: Not reported
- Diet (e.g. ad libitum): ad libitum (Purina chow diet)
- Water (e.g. ad libitum): ad libitum (Not reported to water source)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 to 25 °C
- Humidity (%): 40 to 50 %
- Air changes (per hr): Not reported
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- The doses selected (125–500 mg/kg body weight) were based on the anticlastogenic activity of the highest dose (500 mg/kg body weight) of magnesium sulfate observed in a preliminary study. The different experimental groups of mice consisted of: (1) untreated control (distilled water); (2) magnesium sulphate, 125 mg/kg/day; (3) magnesium sulphate, 250 mg/kg/day; (4) magnesium sulphate, 500 mg/kg/day. Magnesium sulphate was dissolved in water and administered by gavage to mice in groups 2, 3 and 4 for 7 days.
- Duration of treatment / exposure:
- Magnesium sulfate was administered by gavage to mice for 7 days.
- Frequency of treatment:
- once a day
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 125, 250 and 500 mg/kg
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 mice/male/dose
- Control animals:
- yes, concurrent vehicle
Examinations
- Tissues and cell types examined:
- The micronucleus test used to determine the clastogenicity in femoral marrow cells.
- Details of tissue and slide preparation:
- Freshly killed animals, both the femoral were removed in toto and freed from muscles. The femoral marrow cells were aspirated in fetal calf serum as a find suspension and centrifuged. After centrifugation, the cells were spread on slides and air dried. Coded slides were fixed in methanol and stained with Ma-Gruenwald solution followed by Giemsa staining.
- Evaluation criteria:
- Polychromatic erythrocytes (PCE) were screened for micronuclei, and normochromatic erythrocytes (NCE) were recorded. The PCE/NCE ratio was calculated to analyse the bone marrow depression.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
Any other information on results incl. tables
Table 1. Effect of magnesium sulfate on micronuclei in mice
Dose (mg/kg bw) |
Duration of treatment |
PCE Screence |
Mean micronucleated PCE+/-SE, % |
NCE Screened |
Mean PCE/NCE ratio+/-SE |
0 |
12 |
5,127 |
0.29 +/-0.03 |
4,917 |
1.04 +/-0.03 |
24 |
5,120 |
0.27 +/-0.03 |
5,021 |
1.02 +/-0.03 |
|
48 |
5,064 |
0.27 +/-0.04 |
5,049 |
1.00 +/-0.01 |
|
125 |
12 |
5,175 |
0.41 +/-0.05 |
5,256 |
0.99 +/-0.04 |
24 |
5,190 |
0.28 +/-0.02 |
5,129 |
1.01 +/-0.02 |
|
48 |
5,090 |
0.31 +/-0.04 |
5,143 |
0.99 +/-0.02 |
|
250 |
12 |
5,239 |
0.32 +/-0.05 |
5,401 |
0.97 +/-0.04 |
24 |
5,260 |
0.30 +/-0.02 |
5,180 |
1.02 +/-0.04 |
|
48 |
5,242 |
0.28 +/-0.03 |
5,346 |
0.99 +/-0.04 |
|
500 |
12 |
5,483 |
0.33 +/-0.04 |
5,429 |
1.01 +/-0.03 |
24 |
5,300 |
0.30 +/-0.03 |
5,280 |
1.00 +/-0.03 |
|
48 |
5,242 |
0.29 +/-0.03 |
5,260 |
1.00 +/-0.05 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Based on the results, the treatment with magnesium sulfate did not induce any significant changes in the frequency of micronuclei in PCE and the PCE/NCE ratio at different doses and durations of treatment as compared to control.
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