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EC number: 224-675-8 | CAS number: 4443-26-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to internationally accepted guideline. No deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- older version OECD guideline: Daphnia sp., Acute Immobilisation Test and Reproduction Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,3-dihydro-1,3-dioxo-2H-isoindole-2-hexanoic acid
- EC Number:
- 224-675-8
- EC Name:
- 1,3-dihydro-1,3-dioxo-2H-isoindole-2-hexanoic acid
- Cas Number:
- 4443-26-9
- Molecular formula:
- C14H15NO4
- IUPAC Name:
- 6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)hexanoic acid
- Details on test material:
- Lot/Batch number: 5 A 7990
Purity: 99.48%
Water solubility: 200 mg/l
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- HPLC analyses were performed at 0 and 48 h. Test solutions were analysed daily for pH, temperature and oxygen content
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Daily preparation of a stock solution containing 500 mg/l of substance and 0.01% of methylcellulose, used as solubility coadjuvant agent. Solution made with the aid of an ultrasonic device.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Laboratory breeding
- Age at study initiation: less than 24 hours
- Method of breeding: no data
- Feeding during test: no
- Feed during breeding: S. capricornutum
- Amount: not stated
- Frequency: 3 times/week
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 50 mg CaCO3/L
- Test temperature:
- 22 °C
- pH:
- see table
- Dissolved oxygen:
- see table
- Nominal and measured concentrations:
- Nominal: 500 mg/L. HPLC analyses were performed at 0 and 48 h.
But no data of the results present in this study report. - Details on test conditions:
- TEST SYSTEM
- Test vessel: no data
- Type (delete if not applicable): open
- Aeration: no
- No. of organisms per vessel: 5 animals/vessel
- No. of vessels per concentration (replicates): 4 vessels/concentration
- No. of vessels per vehicle control (replicates): 4 vessels water only + 4 vessels water + vehicle
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water from the municipal main system.
OTHER TEST CONDITIONS
- Photoperiod: animals were ketp in the dark during test
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility
TEST CONCENTRATIONS
- Test concentrations: 500 mg/L (limit test)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Details on results:
- No immobilisation observed.
Any other information on results incl. tables
Validity criteria for acute daphnia immobilisation test according to OECD Guideline 202.
|
fulfilled |
Not fullfilled |
Immobilisation of control animals <10% |
YES |
|
Control animals not staying at the surface |
YES |
|
Concentration of dissolved oxygen in all test vessels >3 mg/l |
YES |
|
Concentration of test substance³80% of initial concentration during test |
YES |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see above
- Conclusions:
- The test fulfilled the validity criteria. The test substance is not harmful to D. magna
- Executive summary:
The acute toxicity of PAC in Daphnia magna was studied by means of a static test. 20 animals, divided over 4 replicates, were exposed to 500 mg/l PAC in aqueous 0.01% methylcellulose. Further 2 groups of 20 animals each one were exposed to water only or to the vehicle in water. The exposure lasted for 48 -hours. No immobility was observed. Therefore a concentration of 500 mg/L is a No Observed Effect Concentration.
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