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Diss Factsheets
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EC number: 203-801-5 | CAS number: 110-76-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-ethoxyethylamine
- EC Number:
- 203-801-5
- EC Name:
- 2-ethoxyethylamine
- Cas Number:
- 110-76-9
- Molecular formula:
- C4H11NO
- IUPAC Name:
- 2-ethoxyethylamine
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethoxyethylamin
- Physical state: liquid
- Stability under test conditions: stable
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
A stock solution of 12500 mg/L was prepared by stirring the test substance in deionised water for 5 minutes. The test concentrations were made by diluting the stock solution with deionized water.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: The used test strain of Pseudomonas putida DSM 50026 is purchased on regular times from DSM (Deutsche Sammlung von Mikroorganismen in Braunschweig / German collection of microorganisms in Braunschweig) and cultured further on agar slants in the Laboratory of Ecotoxicology of BASF AG in Ludwigshafen, Germany.
- Method of cultivation: The stock cultures are kept in slant agar culturing tubes of 50 mL. The inoculation density is one smear from an inoculating loop. The culture is transferred weekly.
- Pretreatment: The pre-cultures are kept in Erlenmeyer flasks with a nominal volume of 250 mL. The vessels are filled with 100 mL pre-culture medium and inoculated with a smear from an inoculation loop with 10 TE/F bacteria suspension at 21 ± 1 °C and incubated for 7 h while shaking.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
Test conditions
- Test temperature:
- 21 ±1 °C
- pH:
- 7.0 -10.8
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 19.53, 39.06, 78.13, 625, 625 (pH neutralised), 1250, 2500, 2500 (pH neutralised), 5000, 10000 and 10000 (pH neutralised) mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: closed tubes of glass with flat bottom (nominal volume 50 mL, fill volume: 10 mL) plugged with gas unpermeable teflone caps
- No. of organisms per vessel (inoculum density): 5 FNU
- No. of vessels per concentration (replicates): 4 inoculated replicates and 1 unicolated replicate
- No. of vessels per control (replicates): 4 inoculated replicates and 1 unicolated replicate
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: yes
- Test medium: AK-Medium according to DIN 38412, Part 8
EFFECT PARAMETERS MEASURED
Optical density at a wavelength of 436 nm. Measurements are made at t=16h in all inoculated replicates, whereby the uninoculated replicate of each concentration is used as background measurement. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 82.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks:
- (inhibition of cell multiplication)
- Remarks on result:
- other: non-neutralised test solutions
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 145 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks:
- (inhibition of cell multiplication)
- Remarks on result:
- other: non-neutralised test solutions
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks:
- (inhibition of cell multiplication)
- Remarks on result:
- other: neutralised test solutions
- Details on results:
- The inhibition of bacterial cell multiplication is most likely due to pH effects. The corresponding neutralised samples showed a significantly lower toxicity compared to the non-neutralised samples.
Any other information on results incl. tables
Optical densities of the bacterial cultures at t = 16 h and pH values of inoculated and uninoculated replicates
Concentration (mg/L) |
Optical density (mean, corrected) |
Growth (%control) |
Inhibition (% control) |
pH |
||
uninoculated |
inoculated |
|||||
0 h |
16 h |
(min - max) |
||||
Control |
0.303 |
100.0 |
0.0 |
7.1 |
7.2 |
6.2 - 6.3 |
19.53 |
0.302 |
99.6 |
0.4 |
7.4 |
7.4 |
6.4 - 6.5 |
39.06 |
0.343 |
113.1 |
-13.1 |
7.6 |
7.7 |
6.4 - 7.0 |
78.13 |
0.284 |
93.8 |
6.2 |
8.8 |
8.6 |
6.3 - 6.4 |
156.25 |
0.136 |
44.8 |
55.2 |
9.3 |
9.2 |
6.2 - 6.4 |
312.5 |
0.007 |
2.1 |
97.9 |
9.7 |
9.5 |
9.4 - 9.5 |
625 |
0.007 |
2.0 |
98.0 |
9.9 |
9.7 |
9.8 - 9.8 |
1250 |
0.007 |
2.0 |
98.0 |
10.1 |
10.0 |
10.0 - 10.1 |
2500 |
0.007 |
1.7 |
98.3 |
10.3 |
10.2 |
10.2 - 10.3 |
5000 |
0.009 |
2.2 |
97.8 |
10.5 |
10.4 |
10.5 - 10.5 |
10000 |
0.017 |
2.2 |
97.8 |
10.8 |
10.7 |
10.7 - 10.8 |
625* |
0.161 |
52.8 |
47.2 |
7.2 |
7.2 |
4.3 - 5.0 |
2500* |
0.096 |
31.4 |
68.6 |
7.1 |
7.1 |
4.2 - 4.3 |
10000* |
0.072 |
23.3 |
76.7 |
7.0 |
7.1 |
4.7 - 4.8 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.