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EC number: 202-213-6 | CAS number: 93-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Low mid and high concentration was analysed for quantity of test chemical in exposed concentrations.
Range of average analyzed content of active ingredient (% of nominal)
Plant species Test conc. % recovery
Onion 15, 135 and 1215 81.630 - 93.599
Tomato 5, 45 and 405 92.714 - 96.095
Corn and cabbage 32, 200 and 1250 106.323 - 114.655
Soyabean and sunflower 15, 135 and 1215 98.344 - 106.382
- Sampling method: . Three replicates from low, mid and high dose group, and two replicates from control and vehicle control group samples were processed by transferring 5 grams of test sample in 50 ml of conical flask and 10 ml acetone. and the process was continued by sonicating for 2 minutes. Then the sample were transferred to 15 mL Tarson tube and centrifuged (3000 rpm, at 25°C, 10 min). The supernatant was diluted with acetone, to obtain the final concentration of approximately 0.004 µg/mL of test item and injected to GC-MS/MS.
- Sample storage conditions before analysis: sample was immediately asnalysed soon after sampling - Vehicle:
- yes
- Remarks:
- acetone
- Details on preparation and application of test substrate:
- The artificial substrate was used as the test medium. Acid washed quartz sand (maximum size of 2 mm in diameter) was used as artificial substrate. Within a given phase of test, all the artificial substrate was from the same source.
Maximum Water Holding Capacity of the Soil
Defined quantity of the acid washed quartz sand was loaded into the Keen’s cup (with filter paper in inner chamber). The Keen’s cup was gradually submerged until the water level is above the top of sand. The Keen’s cup was left in the water for three hours. The sand sample was allowed to drain by placing the Keen’s cup onto a filter paper until water draining on to the filter paper was ceased. The Keen’s cup was then weighed followed by drying to constant mass at 105 ± 5 °C. The maximum water holding capacity (WHCmax) was calculated as 26%
- Controls: Artifical soil
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 10 ml of acetone in 2.3kg of substrate
- Evaporation of vehicle before use: yes - Species:
- Allium cepa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: onion
- Plant family: Amaryllidaceae
- Source of seed: Vokkal Seeds Pvt. Ltd - Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: corn
- Plant family: Poaceae (Gramineae)
- Source of seed: Vokkal Seeds Pvt. Ltd. - Species:
- Helianthus annuus
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Sunflower
- Plant family: Asteraceae (Compositae)
- Source of seed: Vokkal Seeds Pvt. Ltd. - Species:
- Brassica oleracea var. capitata
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: cabbage
- Plant family: Brassicaceae (Cruciferae)
- Variety: capitata
- Source of seed: Vokkal Seeds Pvt. Ltd. - Species:
- other: Solanum lycopersicon
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Tomato
- Plant family: Solanaceae
- Source of seed: Vokkal Seeds Pvt. Ltd - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: soybean
- Plant family: Fabaceae (Leguminosae
- Source of seed: Vokkal Seeds Pvt. Ltd. - Test type:
- seedling emergence and seedling growth test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- Onion Tomato: Air temperature range was 19.7 to 22.4 °C
Corn and Cabbage: Air temperature range was 19.6 to 23.0 °C
Soybean and Sunflower: Air temperature range was 19.5 to 23.3 °C - pH:
- 5.51
- Moisture:
- 26% moisture
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Laboratory
- Test container (type, material, size):non porous plastic pots, with diameter: 15.5 cm, surface area about: 189 cm2
- Amount of soil: 2kg/pot
- No. of seeds per container:
Plant species Plants per concentration Plants per pot Pots per concentration Planting depth (mm)
Onion 5 20 4 5
Corn 2 20 10 10
Cabbage 3 21 7 5
Tomato 3 21 7 5
Sunflower 3 21 7 10
Soybean 3 21 7 10
- No. of plants (retained after thinning):
- No. of replicates per treatment group: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per vehicle control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
GROWTH CONDITIONS
- Photoperiod: controlled light-dark cycles with 16 hours light and 8 hours dark
- Light source: PAR light (photosynthetically active radiation) wavelength 400 - 700 nm
- Light intensity and quality: between 22600 to 24100 Lux.
- Relative humidity (%): 71 to 89 %.
-Carbondioxide: 340 to 370 ppm
- Watering regime and schedules: Initial top watering was used to stimulate seed germination. After seedling emergence, bottom and top watering (~ 40 to 50 mL/pot sprinkled over artificial substrate and tray) were used. Frequency of watering as well as volume of water used for watering was dependent on artificial substrate moisture content. At any point during watering, water logging situations was avoided
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Justification for using less concentrations than requested by guideline: test concentrations were used as per test guidelines
- Range finding study
Test concentrations of 10, 100, 500 and 1000 mg/kg dry weight of artificial substrate was tested along with vehicle control during the range finding test.
- Results used to determine the conditions for the definitive study: : growth and development of plant - Nominal and measured concentrations:
- Nominal concentrations
- Reference substance (positive control):
- yes
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 135 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Seed survival
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Seedling Survival
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 135 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot weight
- Species:
- Allium cepa
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 135 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 200 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 250 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 80 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 200 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 200 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 200 ng/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 250 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 250 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 80 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 80 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Brassica oleracea var. capitata
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 200 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: dry shoot weight
- Species:
- other: Solanum lycopersicon
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: dry shoot weight
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 135 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 215 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 135 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 215 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 405 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 215 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seedling survival
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry shoot weight
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 45 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: dry shoot weight
- Details on results:
- SEED GERMINATION in control
- Percent seed germination: 100
SEEDLING EMERGENCE
- Percent seedling emergence: 90
- Percent survival: 100 - Reported statistics and error estimates:
- A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible.
The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
All analyses and comparisons were evaluated at the 5% (p<0.05) level. The unit of expression of endpoints was mg/kg dry weight of artificial substrate. - Validity criteria fulfilled:
- yes
- Remarks:
- • The seedling emergence was at least 90% in all the tested plant species; mean survival of emerged control(s) seedlings 100% • The plants exhibited only normal variation in growth and morphology; • Environmental conditions and growth media are same
- Conclusions:
- Based on the outcomes of definitive test by concerning the emergence, the shoot length, and the dry weight were statistically analyzed to determine the EC10, EC20, EC50, EC90, NOEC and LOEC. The NOEC values based on the emergence, the shoot length, and the dry weight, are 45, 45, 45 (onion), 200, 80, 200 (corn), 200,32,80 (cabbage), 5,15,15 (tomato), 135,15, 135 (soybean) and 405, 15, 15sunflower
- Executive summary:
The study aimed at evaluating the effect of test item on seedling emergence and seedling growth of 6 terrestrial plants was conducted on 2 monocotyledonous and 4 dicotyledonous species. The test item was mixed into the acid washed quartz sand (artificial substrate/soil which was used as the test medium) and exposed as per test guidelines.
A range finding test was carried out with the plant species (onion, corn, cabbage, tomato, soybean, and sunflower) at 10, 100, 500 and 1000 mg test item/kg dry weight of artificial substrate along with vehicle control to determine test concentrations for the definitive test. Effects of test item were observed on seedling emergence, shoot height, and dry shoot weight on day 14 in all the tested plant species.
Based on the range finding results for each plant species, five test concentrations of test item were tested in main study. There was also adequate number of concurrent control and vehicle control (acetone) group. Seeds of the test species were sown in plastic pots with 2 seeds/pot (corn), 3 seeds/pot (cabbage, tomato, soybean, and sunflower) and 5 seeds/pot (onion). Uniform seeds of the test plant species were sown in chemically inert plastic pots containing the test artificial substrate. Within a given phase of the test, all test plants including the control(s) were from the same source. The plants were grown in non-porous plastic pots with a tray saucer under the pot. The pots were large enough to allow normal growth. A pot was defined as the replicate (diameter: 15.5 cm, surface area about: 189 cm2)
To prepare required test item concentration, required quantity of test item was weighed and mixed with acetone (sonication was made, where essential) followed by introduction into artificial substrate. Vehicle was allowed to dry completely, and this was homogenously mixed using blender. For control, required quantity of artificial substrate was mixed thoroughly with nutrient solution. For vehicle control, required quantity of artificial substrate was mixed with acetone and allowed to dry completely and this was homogenously mixed using blender. After test medium preparation, the pots were filled with uniform quantity of test medium (2 kg). This was followed by addition of required volume (220 mL/pot) of water based on WHCmax (26%) of the artificial substrate.
The concentrations were separated by for of 3 for onion soybean sunflower and tomato, and 2.5 for corn and cabbage. The test concentrations for onion, soybean and sunflower are 15, 45, 135, 405, 1215 mg/kg/dw. For corn and cabbage are 32, 80, 200, 500,1250 mg/kg/dw. For tomato are 5, 15, 45, 135, 405 mg/kg/dw. The experiment was conducted in a special room with PAR lights with light intensity22600 to 24100 Lux, and suitable environmental conditions withair temperature of 19.5 to 23.3 °C and air humidity of 71 to 89 %were provided for plant species. During the experiment, the plants were observed for emergence (every day and then at 7 days interval) and visual phytotoxicity (at 7 and 14 days). The experiment completed on 14 days after the emergence of 50% of the vehicle control seedlings. At the end of the experiment, the number of surviving plants, their height and dry weight were observed.
Analytical verification of the test medium (test item active ingredient analysis in test medium) was performed using a validated analytical method to determine the test item. The samples from the low, mid and high-test item concentrations were analyzed at the beginning of the test (day 0) along with the control(s). For analysis, required quantity of test medium was sampled on day 0
During the observation period, i.e. 14 days after 50% of the control(s) plants have emerged, the plants were observed frequently for emergence and visual phytotoxicity and mortality. At the end of the test, measurement of percent emergence and biomass of surviving plants were recorded, as well as visible detrimental effects on different parts of the plant. The latter include abnormalities in appearance of the emerged seedlings, stunted growth, chlorosis, discoloration, mortality, and effects on plant development. A uniform scoring system for visual injury was used to evaluate the observable toxic responses. The final biomass was measured using final average dry shoot weight of surviving plants, by harvesting the shoot at the soil surface and drying them to constant weight at 60 to 70 ºC. The height of the shoot of surviving plants were measured at the end of the test.
Plant observations on days 7 and 14 recorded normal in control and vehicle control. In certain test item treated groups exhibited visual phytotoxicity (toxic responses) of stunted growth and mortality (except onion, cabbage, and tomato). Effects of test item were observed on seedling emergence, seedling survival (except onion, cabbage, and tomato), shoot height, and dry shoot weight on day 14 in all the tested plant species.
Visual assessment is the rating of visual damage based on observations of plant stand, vigour, malformation, chlorosis, necrosis, and overall appearance compared with control(s). Uniform scoring system was used for visual injury to evaluate the observable toxic responses. Plants were observed for severity of the visual phytotoxic effects of test item from the grading scale of 0 to 100 %.
A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.
· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).
· The seedlings didn’t exhibited visible phytotoxic effects and the plants exhibited only normal variation in growth and morphology for particular species (acceptable criterion: seedlings do not exhibit visible phytotoxic effects and the plants exhibit only normal variation in growth and morphology for particular species).
· The mean survival of emerged control(s) seedlings was at least 100% for the duration of the study (acceptable criterion: mean survival of emerged control(s) seedlings should be at least 90% for the duration of the study).
· Environmental conditions for a particular species was identical and growing media contained the same amount of substrate in all the tested plant species from the same source (acceptable criterion: environmental conditions for a particular species should be identical and growing media should contain the same amount of substrate from the same source)
Based on the outcomes of definitive test by concerning the emergence, the shoot length, and the dry weight were statistically analyzed to determine the EC10, EC20, EC50, EC90, NOEC and LOEC. The NOEC values based on the emergence, the shoot length, and the dry weight, are 45, 45, 45 (onion), 200, 80, 200 (corn), 200,32,80 (cabbage), 5,15,15 (tomato), 135,15, 135 (soybean) and 405, 15, 15 sunflower.
Reference
Range finding test:
Plant species: Onion |
|||||||
Test conc. (mg/kg) |
% of Seedling emergence |
% of Seedling survival |
% Shoot height reduction |
% Dry shoot weight reduction |
|||
0 (V) |
80.0 |
100.0 |
- |
- |
|||
10 |
80.0 |
100.0 |
2.0 |
-0.2 |
|||
100 |
80.0 |
100.0 |
2.0 |
-0.7 |
|||
500 |
50.0 |
100.0 |
-45.2 |
-46.8 |
|||
1000 |
30.0 |
100.0 |
-50.7 |
-56.0 |
|||
Plant species:Corn |
|||||||
0 (V) |
75.0 |
100.0 |
- |
- |
|||
10 |
75.0 |
100.0 |
3.3 |
1.8 |
|||
100 |
87.5 |
100.0 |
0.8 |
-1.4 |
|||
500 |
62.5 |
100.0 |
-53.2 |
-52.9 |
|||
1000 |
50.0 |
100.0 |
-78.6 |
-69.8 |
|||
Plant species: Cabbage |
|||||||
Test conc. (mg/kg) |
% of Seedling emergence |
% of Seedling survival |
% Shoot height reduction |
% Dry shoot weight reduction |
|||
0 (V) |
77.8 |
100.0 |
- |
- |
|||
10 |
77.8 |
100.0 |
0.4 |
1.0 |
|||
100 |
100.0 |
100.0 |
-0.9 |
-1.7 |
|||
500 |
11.1 |
100.0 |
-68.1 |
-45.4 |
|||
1000 |
11.1 |
100.0 |
-70.2 |
-49.0 |
|||
Plant species:Tomato |
|||||||
0 (V) |
100.0 |
100.0 |
- |
- |
|||
10 |
100.0 |
100.0 |
3.9 |
1.4 |
|||
100 |
55.6 |
100.0 |
-24.0 |
-51.6 |
|||
500 |
11.1 |
100.0 |
-75.1 |
-79.8 |
|||
1000 |
0.0 |
- |
- |
- |
|||
Plant species: Soybean |
|||||||
0 (V) |
100.0 |
100.0 |
- |
- |
|||
10 |
100.0 |
100.0 |
1.3 |
0.1 |
|||
100 |
100.0 |
100.0 |
1.5 |
-2.7 |
|||
500 |
55.6 |
55.6 |
-75.7 |
-37.5 |
|||
1000 |
22.2 |
22.2 |
-79.0 |
-56.1 |
|||
Plant species: Sunflower |
|||||||
0 (V) |
100.0 |
100.0 |
- |
- |
|||
10 |
100.0 |
100.0 |
1.4 |
0.3 |
|||
100 |
100.0 |
100.0 |
1.6 |
-1.2 |
|||
500 |
88.9 |
88.9 |
-64.7 |
-49.1 |
|||
1000 |
44.4 |
44.4 |
-85.3 |
-59.2 |
Definitive test
Plant species: Onion |
||||
Test conc. (mg/kg) |
% of Seedling emergence |
% of Seedling survival |
% Shoot height reduction |
% Dry shoot weight reduction |
0 |
90.0 |
100.0 |
- |
- |
0 |
90.0 |
100.0 |
- |
- |
15 |
90.0 |
100.0 |
0.9 |
3.1 |
45 |
100.0 |
100.0 |
-19.4 |
-20.9 |
135 |
80.0 |
100.0 |
*-58.7 |
*-56.7 |
405 |
55.0 |
100.0 |
*-89.1 |
*-78.0 |
1215 |
0.0 |
NA |
NA |
NA |
Plant species:Corn |
||||
0 |
90.0 |
100.0 |
- |
- |
0 |
90.0 |
100.0 |
- |
- |
32 |
90.0 |
100.0 |
0.3 |
-0.2 |
80 |
90.0 |
100.0 |
-13.3 |
-9.2 |
200 |
95.0 |
100.0 |
*-35.6 |
-28.8 |
500 |
75.0 |
100.0 |
*-46.8 |
*-44.7 |
1250 |
35.0 |
71.4 |
*-88.0 |
*-88.2 |
Plant species: Cabbage |
||||
0 |
90.5 |
100.0 |
- |
- |
0 |
90.5 |
100.0 |
- |
- |
32 |
100.0 |
100.0 |
-0.1 |
-0.5 |
80 |
95.2 |
100.0 |
*-12.7 |
-16.9 |
200 |
90.5 |
100.0 |
*-34.2 |
*-37.8 |
500 |
42.9 |
100.0 |
*-75.9 |
*-64.4 |
1250 |
19.0 |
100.0 |
*-85.9 |
*-82.3 |
Plant species:Tomato |
||||
0 |
95.2 |
100.0 |
- |
- |
0 |
95.2 |
100.0 |
- |
- |
5 |
95.2 |
100.0 |
0.3 |
-0.2 |
15 |
81.0 |
100.0 |
-17.3 |
-13.6 |
45 |
66.7 |
100.0 |
*-37.8 |
*-37.5 |
135 |
57.1 |
100.0 |
*-71.8 |
*-68.5 |
405 |
23.8 |
100.0 |
*-86.6 |
*-81.9 |
Plant species: Soybean |
||||
0 |
95.2 |
100.0 |
- |
- |
0 |
95.2 |
100.0 |
- |
- |
15 |
95.2 |
100.0 |
-1.7 |
0.4 |
45 |
100.0 |
100.0 |
*-10.8 |
-6.7 |
135 |
95.2 |
100.0 |
*-27.1 |
-24.1 |
405 |
90.5 |
100.0 |
*-56.8 |
*-55.4 |
1215 |
57.1 |
83.3 |
*-94.4 |
*-88.7 |
Plant species: Sunflower |
||||
Test conc. (mg/kg) |
% of Seedling emergence |
% of Seedling survival |
% Shoot height reduction |
% Dry shoot weight reduction |
0 |
100.0 |
100.0 |
- |
- |
0 |
95.2 |
100.0 |
- |
- |
15 |
100.0 |
100.0 |
2.9 |
-1.8 |
45 |
95.2 |
100.0 |
*-33.4 |
*-30.0 |
135 |
95.2 |
100.0 |
*-41.6 |
*-39.3 |
405 |
95.2 |
100.0 |
*-70.6 |
*-67.5 |
1215 |
23.8 |
40.0 |
*-93.8 |
*-88.0 |
Test Item Active Ingredient in Test Medium
Active ingredient |
Range of average analyzed content of active ingredient (% of nominal) |
||
Plant species |
Test conc. |
Day 0 |
|
test item |
Onion |
15, 135 and 1215 |
81.630 - 93.599 |
Tomato |
5, 45 and 405 |
92.714 - 96.095 |
|
Corn and cabbage |
32, 200 and 1250 |
106.323 - 114.655 |
|
Soyabean and sunflower |
15, 135 and 1215 |
98.344 - 106.382 |
TABLE 1. Definitive[c1] [r2] Test Conditions - Air Temperature and Relative Humidity
Plant species: Onion and Tomato
Day |
Air temperature(°C) |
Relative humidity (%) |
||
Minimum |
Maximum |
Minimum |
Maximum |
|
0 |
19.8 |
22.0 |
71 |
86 |
1 |
19.9 |
22.1 |
73 |
88 |
2 |
19.8 |
22.3 |
72 |
87 |
3 |
19.9 |
22.4 |
74 |
89 |
4 |
19.8 |
22.3 |
73 |
87 |
5 |
19.7 |
22.2 |
72 |
86 |
6 |
19.8 |
22.1 |
73 |
88 |
7 |
19.9 |
22.3 |
74 |
87 |
8 |
19.8 |
22.1 |
73 |
86 |
9 |
19.9 |
22.2 |
74 |
88 |
10 |
20.0 |
22.2 |
73 |
87 |
11 |
19.9 |
22.1 |
74 |
86 |
12 |
20.0 |
22.1 |
73 |
88 |
13 |
19.9 |
22.3 |
72 |
87 |
14 |
19.8 |
22.2 |
71 |
86 |
15 |
19.9 |
22.1 |
73 |
87 |
16 |
19.9 |
22.2 |
72 |
89 |
17 |
19.8 |
22.0 |
73 |
86 |
18 |
19.9 |
22.1 |
74 |
85 |
19 |
20.0 |
22.2 |
73 |
84 |
20 |
19.9 |
22.3 |
74 |
86 |
21 |
20.0 |
22.2 |
72 |
85 |
Air temperature range was 19.7 to 22.4 °C and relative humidity range was 71 to 89 %.
Plant species: Corn and Cabbage
Day |
Air temperature(°C) |
Relative humidity (%) |
||
Minimum |
Maximum |
Minimum |
Maximum |
|
0 |
19.9 |
23.0 |
73 |
84 |
1 |
19.8 |
22.1 |
76 |
85 |
2 |
19.6 |
22.9 |
75 |
83 |
3 |
19.7 |
22.5 |
74 |
81 |
4 |
19.6 |
22.3 |
76 |
83 |
5 |
19.7 |
22.2 |
73 |
82 |
6 |
19.8 |
22.7 |
74 |
83 |
7 |
19.6 |
22.0 |
75 |
84 |
8 |
19.7 |
22.3 |
76 |
86 |
9 |
19.8 |
22.5 |
73 |
89 |
10 |
19.6 |
22.9 |
75 |
85 |
11 |
19.7 |
22.2 |
74 |
84 |
12 |
19.6 |
22.9 |
76 |
81 |
13 |
19.8 |
22.6 |
73 |
86 |
14 |
19.7 |
22.6 |
73 |
84 |
15 |
19.9 |
22.7 |
72 |
83 |
16 |
19.6 |
22.8 |
75 |
81 |
17 |
19.8 |
22.9 |
75 |
83 |
18 |
19.6 |
22.7 |
73 |
81 |
19 |
19.8 |
22.2 |
75 |
84 |
Air temperature range was 19.6 to 23.0 °C and relative humidity range was 72 to 89 %.
Plant species: Soybean and Sunflower
Day |
Air temperature(°C) |
Relative humidity (%) |
||
Minimum |
Maximum |
Minimum |
Maximum |
|
0 |
19.9 |
23.3 |
76 |
85 |
1 |
19.6 |
22.3 |
74 |
84 |
2 |
19.8 |
22.5 |
76 |
85 |
3 |
19.7 |
22.6 |
75 |
86 |
4 |
19.6 |
22.4 |
73 |
85 |
5 |
19.7 |
22.3 |
74 |
84 |
6 |
19.8 |
22.5 |
73 |
83 |
7 |
19.5 |
22.3 |
74 |
85 |
8 |
19.7 |
22.6 |
75 |
86 |
9 |
19.6 |
22.5 |
76 |
83 |
10 |
19.6 |
22.6 |
73 |
84 |
11 |
19.5 |
22.4 |
75 |
86 |
12 |
19.7 |
22.1 |
76 |
84 |
13 |
19.8 |
22.3 |
73 |
85 |
14 |
19.6 |
22.7 |
72 |
84 |
15 |
19.5 |
22.7 |
75 |
86 |
16 |
19.7 |
22.7 |
79 |
84 |
17 |
19.9 |
22.9 |
74 |
85 |
18 |
19.7 |
22.8 |
72 |
83 |
19 |
19.6 |
22.3 |
71 |
82 |
Air temperature range was 19.5 to 23.3 °C and relative humidity range was 71 to 86 %.
TABLE 1. Definitive Test Conditions - Carbon Dioxide and Light Intensity[c1] [r2]
Plant species: Onion and Tomato
Day |
Carbon dioxide in air (ppm) |
Light intensity (Lux) range |
|||
0 |
340 |
22700 |
23400 |
22900 |
23600 |
1 |
350 |
22800 |
23600 |
23100 |
23800 |
2 |
350 |
22600 |
23500 |
23200 |
23600 |
3 |
350 |
23100 |
23600 |
22900 |
23400 |
4 |
350 |
23300 |
22700 |
23100 |
22700 |
5 |
350 |
22900 |
22800 |
23200 |
23100 |
6 |
350 |
22700 |
23100 |
22800 |
23200 |
7 |
350 |
22800 |
23100 |
23000 |
22900 |
8 |
350 |
22900 |
23000 |
23100 |
22800 |
9 |
350 |
22900 |
22800 |
23100 |
23200 |
10 |
350 |
22900 |
23000 |
23100 |
23000 |
11 |
350 |
22800 |
22900 |
23000 |
22900 |
12 |
350 |
22900 |
23100 |
23200 |
23000 |
13 |
350 |
22800 |
22900 |
23100 |
23000 |
14 |
350 |
22900 |
23000 |
23100 |
23000 |
15 |
350 |
22900 |
22800 |
23100 |
23000 |
16 |
350 |
23100 |
23000 |
22800 |
23200 |
17 |
350 |
23000 |
22900 |
22800 |
23100 |
18 |
350 |
22900 |
22800 |
23000 |
23100 |
19 |
350 |
22800 |
22900 |
23100 |
23000 |
20 |
350 |
22900 |
22900 |
23200 |
23100 |
21 |
350 |
22800 |
22900 |
23100 |
23000 |
Plant species: Corn and Cabbage
Day |
Carbon dioxide in air (ppm) |
Light intensity (Lux) range |
|||
0 |
370 |
23700 |
23900 |
23800 |
24000 |
1 |
360 |
23500 |
23700 |
23900 |
24100 |
2 |
350 |
22900 |
23100 |
23700 |
24000 |
3 |
350 |
22900 |
23200 |
23100 |
23000 |
4 |
350 |
23000 |
23100 |
23100 |
23200 |
5 |
350 |
23100 |
23000 |
23100 |
23300 |
6 |
350 |
23000 |
23100 |
23200 |
22900 |
7 |
350 |
23000 |
23200 |
23100 |
23300 |
8 |
350 |
23400 |
23200 |
23100 |
23300 |
9 |
350 |
23100 |
23300 |
23400 |
23200 |
10 |
350 |
23400 |
23100 |
23600 |
23500 |
11 |
360 |
23100 |
23000 |
23100 |
23300 |
12 |
350 |
23600 |
23500 |
23200 |
23600 |
13 |
350 |
23500 |
23600 |
23500 |
23100 |
14 |
350 |
23100 |
23000 |
22900 |
23000 |
15 |
350 |
23600 |
23500 |
23100 |
23400 |
16 |
350 |
23200 |
23200 |
23500 |
23100 |
17 |
350 |
23200 |
23100 |
23300 |
23400 |
18 |
350 |
24000 |
23800 |
23900 |
23700 |
19 |
350 |
23700 |
23400 |
23600 |
23500 |
Carbon dioxide in air range was 350 to 370 ppm and light intensity range was 22900 to 24100 Lux.
Plant species: Soybean and Sunflower
Day |
Carbon dioxide in air (ppm) |
Light intensity (Lux) range |
|||
0 |
340 |
23100 |
23400 |
23200 |
23700 |
1 |
360 |
23400 |
22900 |
23100 |
23600 |
2 |
370 |
23500 |
22700 |
23200 |
24100 |
3 |
350 |
23200 |
23100 |
23300 |
23100 |
4 |
350 |
23300 |
23100 |
23200 |
23200 |
5 |
360 |
23100 |
23300 |
23400 |
23200 |
6 |
350 |
23500 |
23300 |
23700 |
23400 |
7 |
350 |
23100 |
23600 |
23500 |
23000 |
8 |
360 |
23000 |
23100 |
23600 |
23200 |
9 |
350 |
23400 |
22900 |
23000 |
23200 |
10 |
350 |
23100 |
23500 |
23600 |
23400 |
11 |
360 |
23100 |
23200 |
23400 |
23100 |
12 |
350 |
23200 |
23600 |
23400 |
23200 |
13 |
350 |
23600 |
23000 |
23200 |
23900 |
14 |
350 |
23400 |
23200 |
23600 |
23100 |
15 |
350 |
23700 |
23500 |
23600 |
23300 |
16 |
360 |
23500 |
23600 |
23500 |
23700 |
17 |
350 |
23300 |
23200 |
23000 |
23400 |
18 |
350 |
23400 |
23200 |
22900 |
23100 |
19 |
350 |
23500 |
23100 |
23300 |
23200 |
Carbon dioxide in air range was 340 to 370 ppm and light intensity range was 22700 to 24100 Lux.
TABLE 1. Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
8-Aug |
9-Aug |
10-Aug |
11-Aug |
12-Aug |
13-Aug |
14-Aug |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||||||
21-Aug |
28-Aug |
21-Aug |
28-Aug |
|||||||||||||
CR1 |
4 |
90.0 |
4 |
18 |
100.0 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
CR2 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
2 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
CR3 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
CR4 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
|||
VR1 |
5 |
90.0 |
5 |
18 |
100.0 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
VR2 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
VR3 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
|||
VR4 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
|||
T1R1 |
5 |
90.0 |
5 |
18 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
T1R2 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
T1R3 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
|||
T1R4[YB3] |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
4 |
4 |
N(4), 0%(4) |
N(4), 0%(4) |
|||
T2R1 |
5 |
100.0 |
5 |
20 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
S(5), 40%(5) |
S(5), 40%(5) |
T2R2 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
T2R3 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
S(5), 25%(5) |
S(5), 25%(5) |
|||
T2R4 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
5 |
5 |
N(5), 0%(5) |
N(5), 0%(5) |
|||
T3R1 |
3 |
80.0 |
3 |
16 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 50%(3) |
S(3), 50%(3) |
T3R2 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
S(5), 50%(5) |
S(5), 50%(5) |
|||
T3R3 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
4 |
4 |
S(4), 60%(3), 90%(1) |
S(4), 60%(3), 90%(1) |
|||
T3R4 |
4 |
4 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
4 |
4 |
S(4), 70%(4) |
S(4), 70%(4) |
|||
T4R1 |
4 |
55.0 |
4 |
11 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
4 |
4 |
S(4), 90%(4) |
S(4), 90%(4) |
T4R2 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 90%(3) |
S(3), 90%(3) |
|||
T4R3 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 90%(2) |
S(2), 90%(2) |
|||
T4R4 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 90%(2) |
S(2), 90%(2) |
|||
T5R1 |
0 |
0.0 |
- |
- |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
T5R2 |
0 |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R3 |
0 |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R4 |
0 |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.
Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Dry shoot weight (g) on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Group dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||||
1 |
2 |
3 |
4 |
5 |
Average |
|||||||
28-Aug |
29-Aug |
|||||||||||
CR1 |
8.5 |
11.0 |
9.7 |
9.8 |
- |
9.8 |
0.0251 |
25.1 |
- |
0.1164 |
0.0065 |
- |
CR2 |
8.8 |
11.2 |
9.6 |
10.4 |
9.6 |
9.9 |
0.0318 |
31.8 |
||||
CR3 |
9.3 |
10.6 |
11.2 |
8.6 |
11.3 |
10.2 |
0.0332 |
33.2 |
||||
CR4 |
9.8 |
9.6 |
8.8 |
11.4 |
- |
9.9 |
0.0263 |
26.3 |
||||
VR1 |
10.3 |
9.8 |
9.5 |
8.6 |
9.6 |
9.6 |
0.0312 |
31.2 |
- |
0.1176 |
0.0065 |
- |
VR2 |
10.6 |
10.3 |
11.3 |
9.3 |
10.7 |
10.4 |
0.0329 |
32.9 |
||||
VR3 |
9.9 |
11.0 |
10.4 |
10.8 |
- |
10.5 |
0.0274 |
27.4 |
||||
VR4 |
8.8 |
10.1 |
8.7 |
10.3 |
- |
9.5 |
0.0261 |
26.1 |
||||
T1R1 |
10.4 |
9.6 |
10.1 |
10.3 |
10.6 |
10.2 |
0.0327 |
32.7 |
0.9[YB3] |
0.1212 |
0.0067 |
3.1 |
T1R2 |
10.7 |
11.0 |
8.6 |
11.3 |
11.2 |
10.6 |
0.0338 |
33.8 |
||||
T1R3 |
9.6 |
9.5 |
9.8 |
10.4 |
- |
9.8 |
0.0276 |
27.6 |
||||
T1R4 |
10.2 |
10.4 |
8.9 |
9.6 |
- |
9.8 |
0.0271 |
27.1 |
||||
T2R1 |
5.8 |
5.5 |
6.0 |
5.9 |
5.4 |
5.7 |
0.0183 |
18.3 |
-19.4 |
0.1034 |
0.0052 |
-20.9 |
T2R2 |
10.3 |
11.0 |
9.8 |
8.4 |
9.2 |
9.7 |
0.0314 |
31.4 |
||||
T2R3 |
7.2 |
7.3 |
7.7 |
7.1 |
6.8 |
7.2 |
0.0238 |
23.8 |
||||
T2R4 |
11.0 |
9.1 |
8.6 |
10.2 |
9.0 |
9.6 |
0.0299 |
29.9 |
||||
T3R1 |
5.5 |
4.8 |
5.0 |
- |
- |
5.1 |
0.0096 |
9.6 |
-58.7* |
0.0453 |
0.0028 |
-56.7* |
T3R2 |
4.6 |
4.9 |
5.1 |
5.0 |
5.4 |
5.0 |
0.0164 |
16.4 |
||||
T3R3 |
1.5 |
4.0 |
4.3 |
3.8 |
- |
3.4 |
0.0104 |
10.4 |
||||
T3R4 |
3.0 |
2.8 |
3.3 |
3.0 |
- |
3.0 |
0.0089 |
8.9 |
||||
T4R1 |
1.2 |
0.9 |
1.1 |
1.4 |
- |
1.2 |
0.0064 |
6.4 |
-89.1* |
0.0158 |
0.0014 |
-78.0* |
T4R2 |
1.0 |
1.2 |
0.8 |
- |
- |
1.0 |
0.0043 |
4.3 |
||||
T4R3 |
1.4 |
0.9 |
- |
- |
- |
1.2 |
0.0029 |
2.9 |
||||
T4R4 |
0.8 |
1.3 |
- |
- |
- |
1.1 |
0.0022 |
2.2 |
||||
T5R1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
T5R2 |
- |
- |
- |
- |
- |
- |
- |
- |
||||
T5R3 |
- |
- |
- |
- |
- |
- |
- |
- |
||||
T5R4 |
- |
- |
- |
- |
- |
- |
- |
- |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
TABLE 1. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
Seedling emergence |
Seedling emergence |
Plant observations |
||||||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||||
18-Sep |
19-Sep |
20-Sep |
21-Sep |
22-Sep |
29-Sep |
06-Oct |
29-Sep |
06-Oct |
||||||
CR1 |
2 |
90.0 |
2 |
18 |
100.0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
CR2 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR3 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR4 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR5 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR6 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR7 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
|||
CR8 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
|||
CR9 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR10 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR1 |
1 |
90.0 |
1 |
18 |
100.0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
VR2 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
|||
VR3 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR4 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR5 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR6 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR7 |
2 |
2 |
0 |
0 |
0 |
1 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR8 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR9 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR10 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal.
Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
||
1 |
2 |
Average |
|||||||
06-Oct |
07-Oct |
||||||||
CR1 |
15.9 |
16.4 |
16.2 |
0.7811 |
781.1 |
- |
6.6223 |
0.3679 |
- |
CR2 |
15.0 |
15.4 |
15.2 |
0.7323 |
732.3 |
||||
CR3 |
16.6 |
15.2 |
15.9 |
0.7531 |
753.1 |
||||
CR4 |
14.6 |
14.4 |
14.5 |
0.7118 |
711.8 |
||||
CR5 |
13.9 |
15.7 |
14.8 |
0.7362 |
736.2 |
||||
CR6 |
13.0 |
12.9 |
13.0 |
0.7034 |
703.4 |
||||
CR7 |
16.1 |
- |
16.1 |
0.3711 |
371.1 |
||||
CR8 |
15.2 |
- |
15.2 |
0.3562 |
356.2 |
||||
CR9 |
14.3 |
14.2 |
14.3 |
0.7339 |
733.9 |
||||
CR10 |
13.8 |
16.1 |
15.0 |
0.7432 |
743.2 |
||||
VR1 |
14.6 |
- |
14.6 |
0.3666 |
366.6 |
- |
6.6615 |
0.3701 |
- |
VR2 |
15.9 |
- |
15.9 |
0.3775 |
377.5 |
||||
VR3 |
14.3 |
13.9 |
14.1 |
0.7372 |
737.2 |
||||
VR4 |
15.9 |
15.4 |
15.7 |
0.7443 |
744.3 |
||||
VR5 |
16.0 |
15.2 |
15.6 |
0.7556 |
755.6 |
||||
VR6 |
14.8 |
14.6 |
14.7 |
0.7118 |
711.8 |
||||
VR7 |
15.2 |
14.6 |
14.9 |
0.7231 |
723.1 |
||||
VR8 |
13.7 |
14.8 |
14.3 |
0.7311 |
731.1 |
||||
VR9 |
16.2 |
15.3 |
15.8 |
0.7465 |
746.5 |
||||
VR10 |
14.5 |
15.1 |
14.8 |
0.7678 |
767.8 |
Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
21-Sep |
22-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||||
29-Sep |
06-Oct |
29-Sep |
06-Oct |
|||||||||||
T1R1 |
1 |
90.0 |
1 |
18 |
100.0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
T1R2 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R3 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R4 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R5 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R6 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R7 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R8 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R9 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R10 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
|||
T2R1 |
2 |
90.0 |
2 |
18 |
100.0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
T2R2 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T2R3 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
|||
T2R4 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 20%(2) |
S(2), 20%(2) |
|||
T2R5 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 20%(2) |
S(2), 20%(2) |
|||
T2R6 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
S(1), 30%(1) |
S(1), 30%(1) |
|||
T2R7 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 60%(1), 30%(1) |
S(2), 60%(1), 30%(1) |
|||
T2R8 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T2R9 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T2R10 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(1), S(1), 0%(1), 20%(1) |
N(1), S(1), 0%(1), 20%(1) |
|||
T3R1 |
2 |
95.0 |
2 |
19 |
100.0 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 20%(1), 50%(1) |
S(2), 20%(1), 50%(1) |
T3R2 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 20%(1), 30%(1) |
S(2), 20%(1), 30%(1) |
|||
T3R3 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 30%(2) |
S(2), 30%(2) |
|||
T3R4 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 10%(1), 40%(1) |
S(2), 10%(1), 40%(1) |
|||
T3R5 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 20%(1), 50%(1) |
S(2), 20%(1), 50%(1) |
|||
T3R6 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 30%(1), 60%(1) |
S(2), 30%(1), 60%(1) |
|||
T3R7 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 30%(1), 50%(1) |
S(2), 30%(1), 50%(1) |
|||
T3R8 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 20%(1), 40%(1) |
S(2), 20%(1), 40%(1) |
|||
T3R9 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 60%(1) |
S(1), 60%(1) |
|||
T3R10 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 30%(1), 40%(1) |
S(2), 30%(1), 40%(1) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.
contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
||
1 |
2 |
Average |
|||||||
06-Oct |
07-Oct |
||||||||
T1R1 |
13.9 |
- |
13.9 |
0.3608 |
360.8 |
0.3[YB3] |
6.6507 |
0.3695 |
-0.2 |
T1R2 |
14.2 |
14.8 |
14.5 |
0.7426 |
742.6 |
||||
T1R3 |
15.3 |
13.7 |
14.5 |
0.7228 |
722.8 |
||||
T1R4 |
16.0 |
13.9 |
15.0 |
0.7263 |
726.3 |
||||
T1R5 |
15.8 |
14.8 |
15.3 |
0.7334 |
733.4 |
||||
T1R6 |
14.7 |
15.5 |
15.1 |
0.7372 |
737.2 |
||||
T1R7 |
15.3 |
15.8 |
15.6 |
0.7469 |
746.9 |
||||
T1R8 |
16.2 |
15.3 |
15.8 |
0.7581 |
758.1 |
||||
T1R9 |
15.3 |
14.9 |
15.1 |
0.7456 |
745.6 |
||||
T1R10 |
16.0 |
- |
16.0 |
0.3770 |
377.0 |
||||
T2R1 |
13.2 |
14.0 |
13.6 |
0.6989 |
698.9 |
-13.3 |
6.0488 |
0.3360 |
-9.2 |
T2R2 |
15.2 |
14.5 |
14.9 |
0.7315 |
731.5 |
||||
T2R3 |
15.0 |
- |
15.0 |
0.3531 |
353.1 |
||||
T2R4 |
11.6 |
12.2 |
11.9 |
0.6583 |
658.3 |
||||
T2R5 |
10.7 |
12.0 |
11.4 |
0.6376 |
637.6 |
||||
T2R6 |
11.0 |
- |
11.0 |
0.2894 |
289.4 |
||||
T2R7 |
11.1 |
6.4 |
8.8 |
0.4565 |
456.5 |
||||
T2R8 |
16.2 |
16.4 |
16.3 |
0.7956 |
795.6 |
||||
T2R9 |
14.5 |
15.4 |
15.0 |
0.7544 |
754.4 |
||||
T2R10 |
13.9 |
11.1 |
12.5 |
0.6735 |
673.5 |
||||
T3R1 |
12.2 |
7.3 |
9.8 |
0.5321 |
532.1 |
-35.6* |
5.0094 |
0.2637 |
-28.8 |
T3R2 |
11.9 |
11.2 |
11.6 |
0.6401 |
640.1 |
||||
T3R3 |
11.0 |
11.4 |
11.2 |
0.6119 |
611.9 |
||||
T3R4 |
9.6 |
12.4 |
11.0 |
0.5993 |
599.3 |
||||
T3R5 |
12.0 |
7.3 |
9.7 |
0.5282 |
528.2 |
||||
T3R6 |
10.6 |
5.0 |
7.8 |
0.3892 |
389.2 |
||||
T3R7 |
11.1 |
8.0 |
9.6 |
0.5126 |
512.6 |
||||
T3R8 |
11.4 |
10.0 |
10.7 |
0.5578 |
557.8 |
||||
T3R9 |
5.5 |
- |
5.5 |
0.1165 |
116.5 |
||||
T3R10 |
9.6 |
10.6 |
10.1 |
0.5217 |
521.7 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
21-Sep |
22-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||||
29-Sep |
06-Oct |
29-Sep |
06-Oct |
|||||||||||
T4R1 |
2 |
75.0 |
2 |
15 |
100.0 |
0 |
0 |
0 |
1 |
1 |
2 |
2 |
S(2), 40%(1), 60%(1) |
S(2), 40%(1), 60%(1) |
T4R2 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 80%(1) |
S(1), 80%(1) |
|||
T4R3 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 80%(1) |
S(1), 80%(1) |
|||
T4R4 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
S(1), 40%(1) |
S(1), 40%(1) |
|||
T4R5 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 40%(1), 60%(1) |
S(2), 40%(1), 60%(1) |
|||
T4R6 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 40%(1) |
S(1), 40%(1) |
|||
T4R7 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 50%(2) |
S(2), 50%(2) |
|||
T4R8 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 40%(2) |
S(2), 40%(2) |
|||
T4R9 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
S(2), 10%(1), 80%(1) |
S(2), 10%(1), 80%(1) |
|||
T4R10 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
S(1), 40%(1) |
S(1), 40%(1) |
|||
T5R1 |
2 |
35.0 |
0 |
5 |
71.4 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 80%(2) |
D(2), 100%(2) |
T5R2 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 90%(1), 80%(1) |
S(2), 90%(1), 80%(1) |
|||
T5R3 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 90%(1) |
S(1), 90%(1) |
|||
T5R4 |
- |
- |
0 |
0 |
0 |
- |
- |
- |
- |
- |
- |
|||
T5R5 |
- |
- |
0 |
0 |
0 |
- |
- |
- |
- |
- |
- |
|||
T5R6 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 80%(1) |
S(1), 80%(1) |
|||
T5R7 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 95%(1) |
S(1), 95%(1) |
|||
T5R8 |
- |
- |
0 |
0 |
0 |
- |
- |
- |
- |
- |
- |
|||
T5R9 |
- |
- |
0 |
0 |
0 |
- |
- |
- |
- |
- |
- |
|||
T5R10 |
- |
- |
0 |
0 |
0 |
- |
- |
- |
- |
- |
- |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.
Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
||
1 |
2 |
Average |
|||||||
06-Oct |
07-Oct |
||||||||
T4R1 |
10.0 |
7.0 |
8.5 |
0.4217 |
421.7 |
-46.8* |
3.0692 |
0.2046 |
-44.7* |
T4R2 |
2.0 |
- |
2.0 |
0.0641 |
64.1 |
||||
T4R3 |
2.4 |
- |
2.4 |
0.0863 |
86.3 |
||||
T4R4 |
11 |
- |
11.0 |
0.2453 |
245.3 |
||||
T4R5 |
7.0 |
11.0 |
9.0 |
0.4701 |
470.1 |
||||
T4R6 |
9.9 |
- |
9.9 |
0.2193 |
219.3 |
||||
T4R7 |
8.3 |
8.5 |
8.4 |
0.3995 |
399.5 |
||||
T4R8 |
11.0 |
9.4 |
10.2 |
0.4867 |
486.7 |
||||
T4R9 |
13.0 |
2.0 |
7.5 |
0.3981 |
398.1 |
||||
T4R10 |
11.0 |
- |
11.0 |
0.2781 |
278.1 |
||||
T5R1 |
- |
- |
- |
- |
- |
-88.0* |
0.2178 |
0.0436 |
-88.2* |
T5R2 |
1.5 |
2.3 |
1.9 |
0.0932 |
93.2 |
||||
T5R3 |
1.7 |
- |
1.7 |
0.0381 |
38.1 |
||||
T5R4 |
- |
- |
- |
- |
- |
||||
T5R5 |
- |
- |
- |
- |
- |
||||
T5R6 |
2.8 |
- |
2.8 |
0.0632 |
63.2 |
||||
T5R7 |
0.8 |
- |
0.8 |
0.0233 |
23.3 |
||||
T5R8 |
- |
- |
- |
- |
- |
||||
T5R9 |
- |
- |
- |
- |
- |
||||
T5R10 |
- |
- |
- |
- |
- |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
TABLE 1. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
21-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
||||||||||
28-Sep |
05-Oct |
28-Sep |
05-Oct |
||||||||||
CR1 |
2 |
90.5 |
2 |
19 |
100.0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
CR2 |
3 |
3 |
0 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR3 |
3 |
3 |
0 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR6 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR7 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR1 |
1 |
90.5 |
1 |
19 |
100.0 |
0 |
0 |
0 |
1 |
1 |
1 |
N(1), 0%(1) |
N(1), 0%(1) |
VR2 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR4 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR5 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R1 |
3 |
100.0 |
3 |
21 |
100.0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
T1R2 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R6 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
1 |
3 |
3 |
S(3), 20%(3) |
S(3), 20%(3) |
T2R2 |
3 |
3 |
0 |
0 |
0 |
2 |
3 |
3 |
S(3), 30%(3) |
S(3), 30%(3) |
|||
T2R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R4 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T2R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R6 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
|||
T2R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.
contd. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Dry shoot weight (g) on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
05-Oct |
06-Oct |
|||||||||
CR1 |
4.2 |
4.0 |
- |
4.1 |
0.0888 |
88.8 |
- |
0.8300 |
0.0437 |
- |
CR2 |
4.3 |
4.7 |
4.2 |
4.4 |
0.1304 |
130.4 |
||||
CR3 |
4.2 |
4.1 |
4.4 |
4.2 |
0.1222 |
122.2 |
||||
CR4 |
4.1 |
4.2 |
4.6 |
4.3 |
0.1342 |
134.2 |
||||
CR5 |
4.2 |
4.4 |
3.9 |
4.2 |
0.1265 |
126.5 |
||||
CR6 |
3.8 |
4.9 |
4.5 |
4.4 |
0.1378 |
137.8 |
||||
CR7 |
4.0 |
3.9 |
- |
4.0 |
0.0901 |
90.1 |
||||
VR1 |
4.7 |
- |
- |
4.7 |
0.0467 |
46.7 |
- |
0.8363 |
0.0440 |
- |
VR2 |
3.8 |
4.1 |
4.4 |
4.1 |
0.1236 |
123.6 |
||||
VR3 |
4.2 |
3.9 |
4.8 |
4.3 |
0.1379 |
137.9 |
||||
VR4 |
3.9 |
3.7 |
4.5 |
4.0 |
0.1194 |
119.4 |
||||
VR5 |
4.8 |
4.8 |
4.9 |
4.8 |
0.1462 |
146.2 |
||||
VR6 |
4.7 |
4.9 |
4.2 |
4.6 |
0.1335 |
133.5 |
||||
VR7 |
4.2 |
4.0 |
3.9 |
4.0 |
0.1290 |
129.0 |
||||
T1R1 |
3.8 |
4.8 |
4.3 |
4.3 |
0.1316 |
131.6 |
-0.1 |
0.9199 |
0.0438 |
-0.5 |
T1R2 |
4.2 |
4.6 |
3.9 |
4.2 |
0.1329 |
132.9 |
||||
T1R3 |
4.4 |
3.9 |
4.8 |
4.4 |
0.1388 |
138.8 |
||||
T1R4 |
4.7 |
4.0 |
4.9 |
4.5 |
0.1372 |
137.2 |
||||
T1R5 |
3.9 |
4.7 |
3.8 |
4.1 |
0.1194 |
119.4 |
||||
T1R6 |
4.2 |
4.6 |
4.9 |
4.6 |
0.1364 |
136.4 |
||||
T1R7 |
4.2 |
4.9 |
4.2 |
4.4 |
0.1236 |
123.6 |
||||
T2R1 |
3.0 |
3.8 |
3.9 |
3.6 |
0.1023 |
102.3 |
-12.7* |
0.7317 |
0.0366 |
-16.9 |
T2R2 |
3.4 |
3.5 |
2.9 |
3.3 |
0.0973 |
97.3 |
||||
T2R3 |
4.1 |
3.9 |
4.3 |
4.1 |
0.1227 |
122.7 |
||||
T2R4 |
4.0 |
4.0 |
- |
4.0 |
0.0822 |
82.2 |
||||
T2R5 |
3.8 |
4.4 |
4.1 |
4.1 |
0.1193 |
119.3 |
||||
T2R6 |
3.8 |
3.9 |
4.0 |
3.9 |
0.0983 |
98.3 |
||||
T2R7 |
3.9 |
3.6 |
3.8 |
3.8 |
0.1096 |
109.6 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
21-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
||||||||||
28-Sep |
05-Oct |
28-Sep |
05-Oct |
||||||||||
T3R1 |
3 |
90.5 |
3 |
19 |
100.0 |
0 |
0 |
0 |
2 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
T3R2 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 40%(2) |
S(2), 40%(2) |
|||
T3R3 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
2 |
S(2), 50%(2) |
S(2), 50%(2) |
|||
T3R4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 20%(2), 50%(1) |
S(3), 20%(2), 50%(1) |
|||
T3R5 |
3 |
3 |
0 |
0 |
0 |
2 |
3 |
3 |
S(3), 30%(3) |
S(3), 30%(3) |
|||
T3R6 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 30%(2), 60%(1) |
S(3), 30%(2), 60%(1) |
|||
T3R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 30%(3) |
S(3), 30%(3) |
|||
T4R1 |
0 |
42.9 |
0 |
9 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
T4R2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T4R3 |
3 |
3 |
0 |
0 |
0 |
2 |
3 |
3 |
S(3), 70%(3) |
S(3), 70%(3) |
|||
T4R4 |
3 |
3 |
0 |
0 |
0 |
0 |
3 |
3 |
S(3), 90%(3) |
S(3), 90%(3) |
|||
T4R5 |
3 |
3 |
0 |
0 |
0 |
1 |
3 |
3 |
S(3), 80%(3) |
S(3), 80%(3) |
|||
T4R6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T4R7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R1 |
0 |
19.0 |
0 |
4 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
T5R2 |
2 |
2 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 95%(2) |
S(2), 95%(2) |
|||
T5R3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R5 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 90%(1) |
S(1), 90%(1) |
|||
T5R6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R7 |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 90%(1) |
S(1), 90%(1) |
Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Dry shoot weight (g) on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
05-Oct |
06-Oct |
|||||||||
T3R1 |
2.9 |
2.6 |
3.3 |
2.9 |
0.0822 |
82.2 |
-34.2* |
0.5203 |
0.0274 |
-37.8* |
T3R2 |
2.9 |
2.6 |
- |
2.8 |
0.0514 |
51.4 |
||||
T3R3 |
2.9 |
2.2 |
- |
2.6 |
0.0496 |
49.6 |
||||
T3R4 |
3.3 |
3.4 |
2.6 |
3.1 |
0.0864 |
86.4 |
||||
T3R5 |
2.9 |
3.0 |
3.2 |
3.0 |
0.0884 |
88.4 |
||||
T3R6 |
3.1 |
2.9 |
2.2 |
2.7 |
0.0744 |
74.4 |
||||
T3R7 |
3.3 |
3.0 |
2.8 |
3.0 |
0.0879 |
87.9 |
||||
T4R1 |
- |
- |
- |
- |
- |
- |
-75.9* |
0.1410 |
0.0157 |
-64.4* |
T4R2 |
- |
- |
- |
- |
- |
- |
||||
T4R3 |
1.6 |
1.8 |
1.3 |
1.6 |
0.0686 |
68.6 |
||||
T4R4 |
0.5 |
0.4 |
0.6 |
0.5 |
0.0251 |
25.1 |
||||
T4R5 |
0.9 |
1.1 |
1.3 |
1.1 |
0.0473 |
47.3 |
||||
T4R6 |
- |
- |
- |
- |
- |
- |
||||
T4R7 |
- |
- |
- |
- |
- |
- |
||||
T5R1 |
- |
- |
- |
- |
- |
- |
-85.9* |
0.0312 |
0.0078 |
-82.3* |
T5R2 |
0.4 |
0.7 |
- |
0.6 |
0.0163 |
16.3 |
||||
T5R3 |
- |
- |
- |
- |
- |
- |
||||
T5R4 |
- |
- |
- |
- |
- |
- |
||||
T5R5 |
0.8 |
- |
- |
0.8 |
0.0088 |
8.8 |
||||
T5R6 |
- |
- |
- |
- |
- |
- |
||||
T5R7 |
0.5 |
- |
- |
0.5 |
0.0061 |
6.1 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
TABLE 1. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
8-Aug |
9-Aug |
10-Aug |
11-Aug |
12-Aug |
13-Aug |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
||||||||||||
20-Aug |
27-Aug |
20-Aug |
27-Aug |
||||||||||||
CR1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
CR2 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR3 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR4 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR5 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
CR6 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR7 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
VR2 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR3 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR4 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR5 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR6 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR7 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
T1R2 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R3 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R4 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R5 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R6 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R7 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R1 |
3 |
81.0 |
3 |
17 |
100.0 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
3 |
S(3), 10%(2), 30%(1) |
S(3), 10%(2), 30%(1) |
T2R2 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(2), 0%(2), S(1), 25%(1) |
N(2), 0%(2), S(1), 25%(1) |
|||
T2R3 |
3 |
3 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R4 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
2 |
N(1), 0%(1), S(1), 35%(1) |
N(1), 0%(1), S(1), 35%(1) |
|||
T2R5 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T2R6 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
2 |
N(1), 0%(1), S(1), 45%(1) |
N(1), 0%(1), S(1), 45%(1) |
|||
T2R7 |
2 |
2 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
2 |
S(2), 30%(2) |
S(2), 30%(2) |
contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
27-Aug |
28-Aug |
|||||||||
CR1 |
11.0 |
10.5 |
10.0 |
10.5 |
0.2212 |
221.2 |
- |
1.4785 |
0.0739 |
- |
CR2 |
10.0 |
10.5 |
10.0 |
10.2 |
0.2311 |
231.1 |
||||
CR3 |
10.5 |
8.0 |
9.5 |
9.3 |
0.2202 |
220.2 |
||||
CR4 |
9.5 |
11.0 |
10.5 |
10.3 |
0.2116 |
211.6 |
||||
CR5 |
9.0 |
8.2 |
- |
8.6 |
0.1347 |
134.7 |
||||
CR6 |
10.5 |
9.5 |
11.0 |
10.3 |
0.2289 |
228.9 |
||||
CR7 |
10.5 |
11.0 |
12.5 |
11.3 |
0.2308 |
230.8 |
||||
VR1 |
10.2 |
9.7 |
8.9 |
9.6 |
0.2191 |
219.1 |
- |
1.4834 |
0.0742 |
- |
VR2 |
9.9 |
10.7 |
- |
10.3 |
0.1404 |
140.4 |
||||
VR3 |
11.0 |
9.9 |
9.6 |
10.2 |
0.2316 |
231.6 |
||||
VR4 |
9.9 |
9.6 |
10.6 |
10.0 |
0.2129 |
212.9 |
||||
VR5 |
10.4 |
10.6 |
9.4 |
10.1 |
0.2197 |
219.7 |
||||
VR6 |
10.9 |
9.7 |
10.7 |
10.4 |
0.2306 |
230.6 |
||||
VR7 |
9.8 |
10.3 |
9.8 |
10.0 |
0.2291 |
229.1 |
||||
T1R1 |
10.3 |
9.9 |
10.2 |
10.1 |
0.2214 |
221.4 |
0.3[YB3] |
1.4809 |
0.0740 |
-0.2 |
T1R2 |
10.1 |
10.2 |
9.5 |
9.9 |
0.2146 |
214.6 |
||||
T1R3 |
10.6 |
10.1 |
- |
10.4 |
0.1469 |
146.9 |
||||
T1R4 |
9.9 |
11.0 |
10.3 |
10.4 |
0.2389 |
238.9 |
||||
T1R5 |
8.9 |
10.6 |
10.5 |
10.0 |
0.2169 |
216.9 |
||||
T1R6 |
10.8 |
9.7 |
9.9 |
10.1 |
0.2214 |
221.4 |
||||
T1R7 |
10.2 |
9.2 |
10.4 |
9.9 |
0.2208 |
220.8 |
||||
T2R1 |
8.1 |
8.0 |
6.8 |
7.6 |
0.1823 |
182.3 |
-17.3 |
1.0889 |
0.0641 |
-13.6 |
T2R2 |
9.2 |
9.4 |
7.1 |
8.6 |
0.2006 |
200.6 |
||||
T2R3 |
9.5 |
9.8 |
10.1 |
9.8 |
0.2246 |
224.6 |
||||
T2R4 |
9.2 |
6.6 |
- |
7.9 |
0.1179 |
117.9 |
||||
T2R5 |
9.7 |
9.3 |
- |
9.5 |
0.1486 |
148.6 |
||||
T2R6 |
9.8 |
6.3 |
- |
8.1 |
0.1144 |
114.4 |
||||
T2R7 |
7.1 |
6.8 |
- |
7.0 |
0.1005 |
100.5 |
contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
8-Aug |
9-Aug |
10-Aug |
11-Aug |
12-Aug |
13-Aug |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
||||||||||||
20-Aug |
27-Aug |
20-Aug |
27-Aug |
||||||||||||
T3R1 |
3 |
66.7 |
3 |
14 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
T3R2 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 50%(2) |
S(2), 50%(2) |
|||
T3R3 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 55%(1) |
S(1), 55%(1) |
|||
T3R4 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
S(1), 50%(1) |
S(1), 50%(1) |
|||
T3R5 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
|||
T3R6 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 50%(2) |
S(2), 50%(2) |
|||
T3R7 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
S(2), 60%(2) |
S(2), 60%(2) |
|||
T4R1 |
3 |
57.1 |
3 |
12 |
100.0 |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
3 |
S(3), 80%(3) |
S(3), 80%(3) |
T4R2 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
- |
S(2), 80%(2) |
|||
T4R3 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 70%(1) |
S(1), 70%(1) |
|||
T4R4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T4R5 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
S(1), 65%(1) |
S(2), 65%(1), 70%(1) |
|||
T4R6 |
3 |
3 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
3 |
S(2), 65%(2) |
S(3), 65%(2), 75%(1) |
|||
T4R7 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 50%(1) |
S(1), 50%(1) |
|||
T5R1 |
0 |
23.8 |
0 |
5 |
100.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
T5R2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R3 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 85%(1) |
S(1), 85%(1) |
|||
T5R4 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
- |
S(1), 90%(1) |
|||
T5R5 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
- |
S(1), 90%(1) |
|||
T5R6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R7 |
2 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
- |
S(2), 85%(2) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth.
Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
27-Aug |
28-Aug |
|||||||||
T3R1 |
5.8 |
5.6 |
4.3 |
5.2 |
0.1146 |
114.6 |
-37.8* |
0.6490 |
0.0464 |
-37.5* |
T3R2 |
6.6 |
7.1 |
- |
6.9 |
0.1006 |
100.6 |
||||
T3R3 |
6.2 |
- |
- |
6.2 |
0.0454 |
45.4 |
||||
T3R4 |
6.8 |
- |
- |
6.8 |
0.0576 |
57.6 |
||||
T3R5 |
5.9 |
6.3 |
6.7 |
6.3 |
0.1396 |
139.6 |
||||
T3R6 |
7.6 |
6.6 |
- |
7.1 |
0.1067 |
106.7 |
||||
T3R7 |
6.0 |
4.9 |
- |
5.5 |
0.0845 |
84.5 |
||||
T4R1 |
2.0 |
2.1 |
2.2 |
2.1 |
0.0661 |
66.1 |
-71.8* |
0.2802 |
0.0234 |
-68.5* |
T4R2 |
1.8 |
1.9 |
- |
1.9 |
0.0243 |
24.3 |
||||
T4R3 |
2.8 |
- |
- |
2.8 |
0.0189 |
18.9 |
||||
T4R4 |
- |
- |
- |
- |
- |
- |
||||
T4R5 |
3.3 |
2.8 |
- |
3.1 |
0.0501 |
50.1 |
||||
T4R6 |
3.2 |
3.4 |
2.3 |
3.0 |
0.0897 |
89.7 |
||||
T4R7 |
4.3 |
- |
- |
4.3 |
0.0311 |
31.1 |
||||
T5R1 |
- |
- |
- |
- |
- |
- |
-86.6* |
0.0673 |
0.0135 |
-81.9* |
T5R2 |
- |
- |
- |
- |
- |
- |
||||
T5R3 |
1.6 |
- |
- |
1.6 |
0.0150 |
15.0 |
||||
T5R4 |
1.3 |
- |
- |
1.3 |
0.0131 |
13.1 |
||||
T5R5 |
1.1 |
- |
- |
1.1 |
0.0110 |
11.0 |
||||
T5R6 |
- |
- |
- |
- |
- |
- |
||||
T5R7 |
1.5 |
1.3 |
- |
1.4 |
0.0282 |
28.2 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
TABLE 1. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||
27-Sep |
04-Oct |
27-Sep |
04-Oct |
|||||||||
CR1 |
2 |
95.2 |
2 |
20 |
100.0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
CR2 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR3 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR4 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR5 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR6 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR7 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR1 |
2 |
95.2 |
2 |
20 |
100.0 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
VR2 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR3 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR4 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR5 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR6 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR7 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
T1R2 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R3 |
2 |
2 |
0 |
0 |
1 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
T1R4 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R5 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R6 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R7 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R1 |
3 |
100.0 |
3 |
21 |
100.0 |
0 |
0 |
2 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
T2R2 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
|||
T2R3 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
|||
T2R4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 15%(3) |
S(3), 15%(3) |
|||
T2R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
|||
T2R6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
|||
T2R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 10%(3) |
S(3), 10%(3) |
. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
04-Oct |
05-Oct |
|||||||||
CR1 |
30.5 |
30.7 |
- |
30.6 |
0.4672 |
467.2 |
- |
4.6632 |
0.2332 |
- |
CR2 |
27.1 |
30.1 |
31.9 |
29.7 |
0.7096 |
709.6 |
||||
CR3 |
30.5 |
29.2 |
28.1 |
29.3 |
0.6998 |
699.8 |
||||
CR4 |
30.6 |
27.8 |
32.5 |
30.3 |
0.7089 |
708.9 |
||||
CR5 |
29.8 |
29.4 |
30.6 |
29.9 |
0.7008 |
700.8 |
||||
CR6 |
31.4 |
30.0 |
28.6 |
30.0 |
0.7000 |
700.0 |
||||
CR7 |
27.9 |
30.3 |
28.6 |
28.9 |
0.6769 |
676.9 |
||||
VR1 |
30.5 |
30.7 |
- |
30.6 |
0.4673 |
467.3 |
- |
4.6945 |
0.2347 |
- |
VR2 |
27.1 |
30.1 |
31.9 |
29.7 |
0.7096 |
709.6 |
||||
VR3 |
30.5 |
29.2 |
28.1 |
29.3 |
0.6999 |
699.9 |
||||
VR4 |
30.6 |
27.8 |
32.5 |
30.3 |
0.7089 |
708.9 |
||||
VR5 |
29.8 |
29.4 |
30.6 |
29.9 |
0.7008 |
700.8 |
||||
VR6 |
31.5 |
30.0 |
28.6 |
30.0 |
0.7210 |
721.0 |
||||
VR7 |
27.9 |
30.3 |
28.6 |
28.9 |
0.6870 |
687.0 |
||||
T1R1 |
30.1 |
29.7 |
28.1 |
29.3 |
0.7104 |
710.4 |
-1.7 |
4.7124 |
0.2356 |
0.4[YB3] |
T1R2 |
28.7 |
30.8 |
30.4 |
30.0 |
0.7223 |
722.3 |
||||
T1R3 |
30.1 |
28.9 |
- |
29.5 |
0.4776 |
477.6 |
||||
T1R4 |
30.2 |
28.8 |
30.1 |
29.7 |
0.7021 |
702.1 |
||||
T1R5 |
28.9 |
29.0 |
28.6 |
28.8 |
0.7016 |
701.6 |
||||
T1R6 |
28.6 |
30.2 |
27.9 |
28.9 |
0.6913 |
691.3 |
||||
T1R7 |
28.9 |
29.1 |
29.0 |
29.0 |
0.7071 |
707.1 |
||||
T2R1 |
25.9 |
26.1 |
26.4 |
26.1 |
0.6400 |
640.0 |
-10.8* |
4.5977 |
0.2189 |
-6.7 |
T2R2 |
25.8 |
25.3 |
25.9 |
25.7 |
0.6220 |
622.0 |
||||
T2R3 |
26.2 |
27.3 |
26.9 |
26.8 |
0.6757 |
675.7 |
||||
T2R4 |
25.1 |
25.2 |
25.9 |
25.4 |
0.6367 |
636.7 |
||||
T2R5 |
26.1 |
28.6 |
28.3 |
27.7 |
0.6806 |
680.6 |
||||
T2R6 |
27.3 |
28.2 |
25.6 |
27.0 |
0.6646 |
664.6 |
||||
T2R7 |
28.3 |
26.8 |
27.6 |
27.6 |
0.6781 |
678.1 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||
27-Sep |
04-Oct |
27-Sep |
04-Oct |
|||||||||
T3R1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
1 |
3 |
3 |
S(3), 30%(3) |
S(3), 30%(3) |
T3R2 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
S(3), 25%(3) |
S(3), 25%(3) |
|||
T3R3 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
S(3), 25%(3) |
S(3), 25%(3) |
|||
T3R4 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
S(3), 30%(3) |
S(3), 30%(3) |
|||
T3R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 25%(3) |
S(3), 25%(3) |
|||
T3R6 |
2 |
2 |
0 |
0 |
2 |
2 |
2 |
S(2), 25%(2) |
S(2), 25%(2) |
|||
T3R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 25%(3) |
S(3), 25%(3) |
|||
T4R1 |
3 |
90.5 |
3 |
19 |
100.0 |
0 |
0 |
0 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
T4R2 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
S(2), 60%(2) |
S(2), 60%(2) |
|||
T4R3 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
|||
T4R4 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
|||
T4R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
|||
T4R6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 60%(3) |
S(3), 60%(3) |
|||
T4R7 |
2 |
2 |
0 |
0 |
1 |
2 |
2 |
S(2), 60%(2) |
S(2), 60%(2) |
|||
T5R1 |
1 |
57.1 |
1 |
10 |
83.3 |
0 |
0 |
0 |
1 |
1 |
S(1), 95%(1) |
S(1), 95%(1) |
T5R2 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 95%(1) |
D(1), 100%(1) |
|||
T5R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 95%(3) |
S(3), 95%(3) |
|||
T5R4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 95%(3) |
S(3), 95%(3) |
|||
T5R5 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 95%(1) |
D(1), 100%(1) |
|||
T5R6 |
2 |
2 |
0 |
0 |
1 |
2 |
2 |
S(2), 95%(2) |
S(2), 95%(2) |
|||
T5R7 |
1 |
1 |
0 |
0 |
0 |
1 |
1 |
S(1), 90%(1) |
S(1), 90%(1) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.
contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
04-Oct |
05-Oct |
|||||||||
T3R1 |
20.6 |
21.0 |
21.2 |
20.9 |
0.5250 |
525.0 |
-27.1* |
3.5653 |
0.1783 |
-24.1 |
T3R2 |
20.8 |
22.8 |
22.5 |
22.0 |
0.5428 |
542.8 |
||||
T3R3 |
21.1 |
22.0 |
23.3 |
22.1 |
0.5446 |
544.6 |
||||
T3R4 |
19.4 |
20.3 |
20.9 |
20.2 |
0.4974 |
497.4 |
||||
T3R5 |
21.0 |
23.1 |
22.8 |
22.3 |
0.5490 |
549.0 |
||||
T3R6 |
22.0 |
22.7 |
- |
22.4 |
0.3632 |
363.2 |
||||
T3R7 |
22.8 |
21.6 |
22.2 |
22.2 |
0.5433 |
543.3 |
||||
T4R1 |
12.3 |
12.5 |
12.6 |
12.5 |
0.3076 |
307.6 |
-56.8* |
1.9911 |
0.1048 |
-55.4* |
T4R2 |
12.4 |
13.6 |
- |
13.0 |
0.2000 |
200.0 |
||||
T4R3 |
12.6 |
13.1 |
13.9 |
13.2 |
0.3248 |
324.8 |
||||
T4R4 |
11.6 |
12.1 |
12.4 |
12.0 |
0.2964 |
296.4 |
||||
T4R5 |
12.5 |
13.7 |
13.6 |
13.3 |
0.3271 |
327.1 |
||||
T4R6 |
13.1 |
13.5 |
12.3 |
13.0 |
0.3195 |
319.5 |
||||
T4R7 |
13.6 |
12.9 |
- |
13.3 |
0.2157 |
215.7 |
||||
T5R1 |
1.3 |
- |
- |
1.3 |
0.0209 |
20.9 |
-94.4* |
0.2661 |
0.0266 |
-88.7* |
T5R2 |
- |
- |
- |
- |
- |
- |
||||
T5R3 |
1.5 |
1.2 |
1.9 |
1.5 |
0.0777 |
77.7 |
||||
T5R4 |
1.6 |
1.9 |
1.9 |
1.8 |
0.0879 |
87.9 |
||||
T5R5 |
- |
- |
- |
- |
- |
- |
||||
T5R6 |
1.1 |
2.0 |
- |
1.6 |
0.0506 |
50.6 |
||||
T5R7 |
2.2 |
- |
- |
2.2 |
0.0290 |
29.0 |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
TABLE 1. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||
27-Sep |
04-Oct |
27-Sep |
04-Oct |
|||||||||
CR1 |
3 |
100.0 |
3 |
21 |
100.0 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
CR2 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR3 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR4 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR5 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
CR7 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
VR2 |
2 |
2 |
0 |
0 |
2 |
2 |
2 |
N(2), 0%(2) |
N(2), 0%(2) |
|||
VR3 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR4 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR5 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR6 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
VR7 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R1 |
3 |
100.0 |
3 |
21 |
100.0 |
0 |
0 |
1 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
T1R2 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R3 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R4 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R5 |
3 |
3 |
0 |
0 |
3 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R6 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T1R7 |
3 |
3 |
0 |
0 |
2 |
3 |
3 |
N(3), 0%(3) |
N(3), 0%(3) |
|||
T2R1 |
2 |
95.2 |
2 |
20 |
100.0 |
0 |
0 |
2 |
2 |
2 |
S(2), 40%(2) |
S(2), 40%(2) |
T2R2 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
|||
T2R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
|||
T2R4 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 30%(2), 40%(1) |
S(3), 30%(2), 40%(1) |
|||
T2R5 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 35%(3) |
S(3), 35%(3) |
|||
T2R6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 35%(3) |
S(3), 35%(3) |
|||
T2R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 35%(3) |
S(3), 35%(3) |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.
contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Total no. of seeds emerged |
% of Seedling emergence |
Seedlings survival on day 14 |
Total no. of seedlings survival on day 14 |
% of Seedling survival |
18-Sep |
19-Sep |
20-Sep |
Seedling emergence |
Plant observations |
||
*Day 7 |
*Day 14 |
*Day 7 |
*Day 14 |
|||||||||
27-Sep |
04-Oct |
27-Sep |
04-Oct |
|||||||||
T3R1 |
3 |
95.2 |
3 |
20 |
100.0 |
0 |
0 |
0 |
3 |
3 |
S(3), 45%(3) |
S(3), 45%(3) |
T3R2 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
|||
T3R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 50%(3) |
S(3), 50%(3) |
|||
T3R4 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
S(2), 50%(2) |
S(2), 50%(2) |
|||
T3R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
|||
T3R6 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 50%(3) |
S(3), 50%(3) |
|||
T3R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 40%(3) |
S(3), 40%(3) |
|||
T4R1 |
2 |
95.2 |
2 |
20 |
100.0 |
0 |
0 |
0 |
2 |
2 |
S(2), 70%(2) |
S(2), 70%(2) |
T4R2 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 70%(2), 80%(1) |
S(3), 70%(2), 80%(1) |
|||
T4R3 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 70%(3) |
S(3), 70%(3) |
|||
T4R4 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 70%(2), 80%(1) |
S(3), 70%(2), 80%(1) |
|||
T4R5 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 70%(2), 80%(1) |
S(3), 70%(2), 80%(1) |
|||
T4R6 |
3 |
3 |
0 |
0 |
1 |
3 |
3 |
S(3), 70%(3) |
S(3), 70%(3) |
|||
T4R7 |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
S(3), 70%(2), 80%(1) |
S(3), 70%(2), 80%(1) |
|||
T5R1 |
2 |
23.8 |
0 |
2 |
40.0 |
0 |
0 |
0 |
2 |
2 |
S(2), 95%(2) |
D(2), 100%(2) |
T5R2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R3 |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
S(2), 95%(2) |
S(2), 95%(2) |
|||
T5R4 |
1 |
0 |
0 |
0 |
0 |
1 |
1 |
S(1), 95%(1) |
D(1), 100%(1) |
|||
T5R5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
|||
T5R7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
- |
- |
Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.
contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
04-Oct |
05-Oct |
|||||||||
CR1 |
19.6 |
21.9 |
22.2 |
21.2 |
0.6017 |
601.7 |
- |
4.3684 |
0.2080 |
- |
CR2 |
20.8 |
21.4 |
19.4 |
20.5 |
0.6269 |
626.9 |
||||
CR3 |
20.1 |
20.2 |
20.2 |
20.2 |
0.6105 |
610.5 |
||||
CR4 |
20.9 |
22.8 |
21.8 |
21.8 |
0.6355 |
635.5 |
||||
CR5 |
19.7 |
20.2 |
20.5 |
20.1 |
0.6334 |
633.4 |
||||
CR6 |
20.5 |
20.6 |
22.5 |
21.2 |
0.6261 |
626.1 |
||||
CR7 |
21.8 |
24.2 |
19.4 |
21.8 |
0.6343 |
634.3 |
||||
VR1 |
18.8 |
18.6 |
18.3 |
18.6 |
0.5876 |
587.6 |
- |
4.1870 |
0.2094 |
- |
VR2 |
19.4 |
19.9 |
- |
19.7 |
0.4564 |
456.4 |
||||
VR3 |
21.2 |
22.2 |
17.9 |
20.4 |
0.6211 |
621.1 |
||||
VR4 |
20.6 |
21.4 |
18.1 |
20.0 |
0.6198 |
619.8 |
||||
VR5 |
22.0 |
19.5 |
21.5 |
21.0 |
0.6378 |
637.8 |
||||
VR6 |
20.5 |
19.9 |
20.6 |
20.3 |
0.6276 |
627.6 |
||||
VR7 |
19.6 |
21.6 |
22.0 |
21.1 |
0.6367 |
636.7 |
||||
T1R1 |
19.2 |
19.6 |
22.6 |
20.5 |
0.5979 |
597.9 |
2.9[YB3] |
4.3189 |
0.2057 |
-1.8 |
T1R2 |
19.6 |
17.4 |
18.7 |
18.6 |
0.5869 |
586.9 |
||||
T1R3 |
20.3 |
21.1 |
19.9 |
20.4 |
0.6006 |
600.6 |
||||
T1R4 |
17.6 |
20.8 |
22.0 |
20.1 |
0.5987 |
598.7 |
||||
T1R5 |
19.6 |
21.9 |
21.8 |
21.1 |
0.6345 |
634.5 |
||||
T1R6 |
22.7 |
22.1 |
20.9 |
21.9 |
0.6459 |
645.9 |
||||
T1R7 |
22.9 |
22.6 |
22.4 |
22.6 |
0.6544 |
654.4 |
||||
T2R1 |
12.6 |
13.1 |
- |
12.9 |
0.2809 |
280.9 |
-33.4* |
2.9307 |
0.1465 |
-30.0* |
T2R2 |
12.4 |
14.8 |
12.3 |
13.2 |
0.4198 |
419.8 |
||||
T2R3 |
12.6 |
13.9 |
12.6 |
13.0 |
0.4227 |
422.7 |
||||
T2R4 |
14.2 |
14.7 |
12.9 |
13.9 |
0.4724 |
472.4 |
||||
T2R5 |
13.5 |
13.6 |
14.5 |
13.9 |
0.4632 |
463.2 |
||||
T2R6 |
13.6 |
13.9 |
13.5 |
13.7 |
0.4398 |
439.8 |
||||
T2R7 |
13.1 |
13.5 |
13.9 |
13.5 |
0.4319 |
431.9 |
contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]
Group & Replicate |
Shoot height on day 14 |
Shoot weight on day 14 |
Dry shoot weight (mg) on day 14 |
% Shoot height reduction on day 14 |
Dry shoot weight (g) on day 14 |
Average dry shoot weight (g) on day 14 |
% Dry shoot weight reduction on day 14 |
|||
1 |
2 |
3 |
Average |
|||||||
04-Oct |
05-Oct |
|||||||||
T3R1 |
12.6 |
12.4 |
12.1 |
12.4 |
0.4081 |
408.1 |
-41.6* |
2.5427 |
0.1271 |
-39.3* |
T3R2 |
13.6 |
12.9 |
12.6 |
13.0 |
0.3987 |
398.7 |
||||
T3R3 |
10.8 |
10.1 |
11.3 |
10.7 |
0.3763 |
376.3 |
||||
T3R4 |
11.6 |
10.1 |
- |
10.9 |
0.2574 |
257.4 |
||||
T3R5 |
12.9 |
12.1 |
12.6 |
12.5 |
0.3898 |
389.8 |
||||
T3R6 |
10.3 |
10.9 |
9.5 |
10.2 |
0.3339 |
333.9 |
||||
T3R7 |
12.3 |
12.9 |
12.7 |
12.6 |
0.3785 |
378.5 |
||||
T4R1 |
6.3 |
6.8 |
- |
6.6 |
0.1513 |
151.3 |
-70.6* |
1.3591 |
0.0680 |
-67.5* |
T4R2 |
6.2 |
4.6 |
6.8 |
5.9 |
0.2119 |
211.9 |
||||
T4R3 |
6.2 |
6.1 |
5.1 |
5.8 |
0.2017 |
201.7 |
||||
T4R4 |
6.4 |
6.6 |
4.1 |
5.7 |
0.1983 |
198.3 |
||||
T4R5 |
6.5 |
4.2 |
6.4 |
5.7 |
0.1901 |
190.1 |
||||
T4R6 |
6.1 |
6.2 |
6.8 |
6.4 |
0.2231 |
223.1 |
||||
T4R7 |
6.8 |
5.0 |
4.5 |
5.4 |
0.1827 |
182.7 |
||||
T5R1 |
- |
- |
- |
- |
- |
- |
-93.8* |
0.0501 |
0.0251 |
-88.0* |
T5R2 |
- |
- |
- |
- |
- |
- |
||||
T5R3 |
1.1 |
1.4 |
- |
1.3 |
0.0501 |
50.1 |
||||
T5R4 |
- |
- |
- |
- |
- |
- |
||||
T5R5 |
- |
- |
- |
- |
- |
- |
||||
T5R6 |
- |
- |
- |
- |
- |
- |
||||
T5R7 |
- |
- |
- |
- |
- |
- |
*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.
Description of key information
The study aimed at evaluating the effect of test item on seedling emergence and seedling growth of 6 terrestrial plants was conducted on 2 monocotyledonous and 4 dicotyledonous species. The test item was mixed into the acid washed quartz sand (artificial substrate/soil which was used as the test medium) and exposed as per test guidelines.
A range finding test was carried out with the plant species (onion, corn, cabbage, tomato, soybean, and sunflower) at 10, 100, 500 and 1000 mg test item/kg dry weight of artificial substrate along with vehicle control to determine test concentrations for the definitive test. Effects of test item were observed on seedling emergence, shoot height, and dry shoot weight on day 14 in all the tested plant species.
Based on the range finding results for each plant species, five test concentrations of test item were tested in main study. There was also adequate number of concurrent control and vehicle control (acetone) group. Seeds of the test species were sown in plastic pots with 2 seeds/pot (corn), 3 seeds/pot (cabbage, tomato, soybean, and sunflower) and 5 seeds/pot (onion). Uniform seeds of the test plant species were sown in chemically inert plastic pots containing the test artificial substrate. Within a given phase of the test, all test plants including the control(s) were from the same source. The plants were grown in non-porous plastic pots with a tray saucer under the pot. The pots were large enough to allow normal growth. A pot was defined as the replicate (diameter: 15.5 cm, surface area about: 189 cm2)
To prepare required test item concentration, required quantity of test item was weighed and mixed with acetone (sonication was made, where essential) followed by introduction into artificial substrate. Vehicle was allowed to dry completely, and this was homogenously mixed using blender. For control, required quantity of artificial substrate was mixed thoroughly with nutrient solution. For vehicle control, required quantity of artificial substrate was mixed with acetone and allowed to dry completely and this was homogenously mixed using blender. After test medium preparation, the pots were filled with uniform quantity of test medium (2 kg). This was followed by addition of required volume (220 mL/pot) of water based on WHCmax (26%) of the artificial substrate.
The concentrations were separated by for of 3 for onion soybean sunflower and tomato, and 2.5 for corn and cabbage. The test concentrations for onion, soybean and sunflower are 15, 45, 135, 405, 1215 mg/kg/dw. For corn and cabbage are 32, 80, 200, 500,1250 mg/kg/dw. For tomato are 5, 15, 45, 135, 405 mg/kg/dw. The experiment was conducted in a special room with PAR lights with light intensity22600 to 24100 Lux, and suitable environmental conditions withair temperature of 19.5 to 23.3 °C and air humidity of 71 to 89 %were provided for plant species. During the experiment, the plants were observed for emergence (every day and then at 7 days interval) and visual phytotoxicity (at 7 and 14 days). The experiment completed on 14 days after the emergence of 50% of the vehicle control seedlings. At the end of the experiment, the number of surviving plants, their height and dry weight were observed.
Analytical verification of the test medium (test item active ingredient analysis in test medium) was performed using a validated analytical method to determine the test item. The samples from the low, mid and high-test item concentrations were analyzed at the beginning of the test (day 0) along with the control(s). For analysis, required quantity of test medium was sampled on day 0
During the observation period, i.e. 14 days after 50% of the control(s) plants have emerged, the plants were observed frequently for emergence and visual phytotoxicity and mortality. At the end of the test, measurement of percent emergence and biomass of surviving plants were recorded, as well as visible detrimental effects on different parts of the plant. The latter include abnormalities in appearance of the emerged seedlings, stunted growth, chlorosis, discoloration, mortality, and effects on plant development. A uniform scoring system for visual injury was used to evaluate the observable toxic responses. The final biomass was measured using final average dry shoot weight of surviving plants, by harvesting the shoot at the soil surface and drying them to constant weight at 60 to 70 ºC. The height of the shoot of surviving plants were measured at the end of the test.
Plant observations on days 7 and 14 recorded normal in control and vehicle control. In certain test item treated groups exhibited visual phytotoxicity (toxic responses) of stunted growth and mortality (except onion, cabbage, and tomato). Effects of test item were observed on seedling emergence, seedling survival (except onion, cabbage, and tomato), shoot height, and dry shoot weight on day 14 in all the tested plant species.
Visual assessment is the rating of visual damage based on observations of plant stand, vigour, malformation, chlorosis, necrosis, and overall appearance compared with control(s). Uniform scoring system was used for visual injury to evaluate the observable toxic responses. Plants were observed for severity of the visual phytotoxic effects of test item from the grading scale of 0 to 100 %.
A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.
· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).
· The seedlings didn’t exhibited visible phytotoxic effects and the plants exhibited only normal variation in growth and morphology for particular species (acceptable criterion: seedlings do not exhibit visible phytotoxic effects and the plants exhibit only normal variation in growth and morphology for particular species).
· The mean survival of emerged control(s) seedlings was at least 100% for the duration of the study (acceptable criterion: mean survival of emerged control(s) seedlings should be at least 90% for the duration of the study).
· Environmental conditions for a particular species was identical and growing media contained the same amount of substrate in all the tested plant species from the same source (acceptable criterion: environmental conditions for a particular species should be identical and growing media should contain the same amount of substrate from the same source)
Based on the outcomes of definitive test by concerning the emergence, the shoot length, and the dry weight were statistically analyzed to determine the EC10, EC20, EC50, EC90, NOEC and LOEC. The NOEC values based on the emergence, the shoot length, and the dry weight, are 45, 45, 45 (onion), 200, 80, 200 (corn), 200,32,80 (cabbage), 5,15,15 (tomato), 135,15, 135 (soybean) and 405, 15, 15 sunflower.
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 5 mg/kg soil dw
Additional information
The study aimed at evaluating the effect of test item on seedling emergence and seedling growth of 6 terrestrial plants was conducted on 2 monocotyledonous and 4 dicotyledonous species. The test item was mixed into the acid washed quartz sand (artificial substrate/soil which was used as the test medium) and exposed as per test guidelines.
A range finding test was carried out with the plant species (onion, corn, cabbage, tomato, soybean, and sunflower) at 10, 100, 500 and 1000 mg test item/kg dry weight of artificial substrate along with vehicle control to determine test concentrations for the definitive test. Effects of test item were observed on seedling emergence, shoot height, and dry shoot weight on day 14 in all the tested plant species.
Based on the range finding results for each plant species, five test concentrations of test item were tested in main study. There was also adequate number of concurrent control and vehicle control (acetone) group. Seeds of the test species were sown in plastic pots with 2 seeds/pot (corn), 3 seeds/pot (cabbage, tomato, soybean, and sunflower) and 5 seeds/pot (onion). Uniform seeds of the test plant species were sown in chemically inert plastic pots containing the test artificial substrate. Within a given phase of the test, all test plants including the control(s) were from the same source. The plants were grown in non-porous plastic pots with a tray saucer under the pot. The pots were large enough to allow normal growth. A pot was defined as the replicate (diameter: 15.5 cm, surface area about: 189 cm2)
To prepare required test item concentration, required quantity of test item was weighed and mixed with acetone (sonication was made, where essential) followed by introduction into artificial substrate. Vehicle was allowed to dry completely, and this was homogenously mixed using blender. For control, required quantity of artificial substrate was mixed thoroughly with nutrient solution. For vehicle control, required quantity of artificial substrate was mixed with acetone and allowed to dry completely and this was homogenously mixed using blender. After test medium preparation, the pots were filled with uniform quantity of test medium (2 kg). This was followed by addition of required volume (220 mL/pot) of water based on WHCmax (26%) of the artificial substrate.
The concentrations were separated by for of 3 for onion soybean sunflower and tomato, and 2.5 for corn and cabbage. The test concentrations for onion, soybean and sunflower are 15, 45, 135, 405, 1215 mg/kg/dw. For corn and cabbage are 32, 80, 200, 500,1250 mg/kg/dw. For tomato are 5, 15, 45, 135, 405 mg/kg/dw. The experiment was conducted in a special room with PAR lights with light intensity22600 to 24100 Lux, and suitable environmental conditions withair temperature of 19.5 to 23.3 °C and air humidity of 71 to 89 %were provided for plant species. During the experiment, the plants were observed for emergence (every day and then at 7 days interval) and visual phytotoxicity (at 7 and 14 days). The experiment completed on 14 days after the emergence of 50% of the vehicle control seedlings. At the end of the experiment, the number of surviving plants, their height and dry weight were observed.
Analytical verification of the test medium (test item active ingredient analysis in test medium) was performed using a validated analytical method to determine the test item. The samples from the low, mid and high-test item concentrations were analyzed at the beginning of the test (day 0) along with the control(s). For analysis, required quantity of test medium was sampled on day 0
During the observation period, i.e. 14 days after 50% of the control(s) plants have emerged, the plants were observed frequently for emergence and visual phytotoxicity and mortality. At the end of the test, measurement of percent emergence and biomass of surviving plants were recorded, as well as visible detrimental effects on different parts of the plant. The latter include abnormalities in appearance of the emerged seedlings, stunted growth, chlorosis, discoloration, mortality, and effects on plant development. A uniform scoring system for visual injury was used to evaluate the observable toxic responses. The final biomass was measured using final average dry shoot weight of surviving plants, by harvesting the shoot at the soil surface and drying them to constant weight at 60 to 70 ºC. The height of the shoot of surviving plants were measured at the end of the test.
Plant observations on days 7 and 14 recorded normal in control and vehicle control. In certain test item treated groups exhibited visual phytotoxicity (toxic responses) of stunted growth and mortality (except onion, cabbage, and tomato). Effects of test item were observed on seedling emergence, seedling survival (except onion, cabbage, and tomato), shoot height, and dry shoot weight on day 14 in all the tested plant species.
Visual assessment is the rating of visual damage based on observations of plant stand, vigour, malformation, chlorosis, necrosis, and overall appearance compared with control(s). Uniform scoring system was used for visual injury to evaluate the observable toxic responses. Plants were observed for severity of the visual phytotoxic effects of test item from the grading scale of 0 to 100 %.
A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.
· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).
· The seedlings didn’t exhibited visible phytotoxic effects and the plants exhibited only normal variation in growth and morphology for particular species (acceptable criterion: seedlings do not exhibit visible phytotoxic effects and the plants exhibit only normal variation in growth and morphology for particular species).
· The mean survival of emerged control(s) seedlings was at least 100% for the duration of the study (acceptable criterion: mean survival of emerged control(s) seedlings should be at least 90% for the duration of the study).
· Environmental conditions for a particular species was identical and growing media contained the same amount of substrate in all the tested plant species from the same source (acceptable criterion: environmental conditions for a particular species should be identical and growing media should contain the same amount of substrate from the same source)
Based on the outcomes of definitive test by concerning the emergence, the shoot length, and the dry weight were statistically analyzed to determine the EC10, EC20, EC50, EC90, NOEC and LOEC. The NOEC values based on the emergence, the shoot length, and the dry weight, are 45, 45, 45 (onion), 200, 80, 200 (corn), 200,32,80 (cabbage), 5,15,15 (tomato), 135,15, 135 (soybean) and 405, 15, 15 sunflower.
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