Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-377-7 | CAS number: 2136-89-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- The study was conducted prior to adoption of the LLNA assay.
Test material
- Reference substance name:
- α,α,α,2-tetrachlorotoluene
- EC Number:
- 218-377-7
- EC Name:
- α,α,α,2-tetrachlorotoluene
- Cas Number:
- 2136-89-2
- Molecular formula:
- C7H4Cl4
- IUPAC Name:
- 1-chloro-2-(trichloromethyl)benzene
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: D. Hall Burton on Trent
- Females (if applicable) nulliparous and non-pregnant: [yes
- Microbiological status of animals, when known:
- Age at study initiation: <1 year old
- Weight at study initiation: 389-493g
- Housing: 5 per cage: aluminium with grid floor above peatmoss filled tray
- Diet (e.g. ad libitum): FD1 guineapig Diet, supplemted with Hay, ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: at least 7 days
- Indication of any skin lesions:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21C
- Humidity (%): 50%
- Air changes (per hr): N/A
- Photoperiod (hrs dark / hrs light): 12/12 (0700-1900)
- IN-LIFE DATES: From: To:
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- paraffin oil
- Concentration / amount:
- 10% w/w
- Day(s)/duration:
- single dose
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
- Route:
- epicutaneous, occlusive
- Vehicle:
- paraffin oil
- Concentration / amount:
- 25% w/w
- Day(s)/duration:
- 48 hours
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- paraffin oil
- Concentration / amount:
- 25%
- Day(s)/duration:
- 24 hours
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 20 (twenty)
- Details on study design:
- RANGE FINDING TESTS:
Induction:
2 guinea pigs intradermal injections of 10%, 5%, 2% and 1% w/v in paraffin oil, assessed for irritation at 24, 48 and 72 hours
2 guinea pigs topical application of 25%,10%, 5% and 2% w/v in paraffin oil., assessed for irritation 24 and 48 hours after patch removal
Challenge: test material applied to shaved flanks at 25% and 10% w/v for 24hrs (occlusive dressing). Reactions assessed 24hours after patch removal.
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: two, one intrademal, one epicutaneous spaced 6 days apart
- Exposure period: 24 hours
- Test groups: one group of 20 animals
- Control group: one group of 20 animals
- Site: flank
- Frequency of applications: every six days
- Duration: 48 hours
- Concentrations: 10% w/v intradermal, 25% epicutaneous
B. CHALLENGE EXPOSURE
- No. of exposures: one
- Day(s) of challenge: Day 14
- Exposure period: 24 hours
- Test groups: one group of 20 animals (25% w/v)
- Control group: one group of 10 animals
- Site: left flank
- Concentrations: 25% w/v in paraffin oil
- Evaluation (hr after challenge): 24 and 48 hours after patch removal
OTHER: - Challenge controls:
- vehicle and test material in non-induced animals
- Positive control substance(s):
- no
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0 % w/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 25% w/v
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 0% w/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 25% w/v
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- not given
- No. with + reactions:
- 13
- Total no. in group:
- 20
- Remarks on result:
- positive indication of skin sensitisation
Any other information on results incl. tables
Dose rangefinding Induction:
No irritation was noted at sites injected with the test substance. 10% w/v was selected for the injection phase of induction.
No irritation was oted at sites treated topically with the test material. 25% w/v was selected for the topical appication phase of induction.
Main test-induction
slight to moderate irritation was noted in the test group and slight irritaiton noted in the control group.
Dose rangefinding -challenge
No irritation was noted at sites treated topically with the test material at 25% and 10% w/v in paraffin oil. 25% w/v was selected for the challenge dose.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- o-Chlorobenzotrichloride does not induce sensitisation in guinea-pig skin under the conditions of the performed study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.