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EC number: 213-268-0 | CAS number: 933-48-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 202, part 2 (1984)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,3,5-trimethylcyclohexanol
- EC Number:
- 204-122-7
- EC Name:
- 3,3,5-trimethylcyclohexanol
- Cas Number:
- 116-02-9
- IUPAC Name:
- 3,3,5-trimethylcyclohexanol
- Details on test material:
- 3,3,5-trimethylcyclohexanol of Hüls AG, purity 98.7 % (GC-FID area). Produced 21 August 1992; Sample No. 1346/920925; Sample ID 3633/81 458
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- MONITORING OF TEST SUBSTANCE CONCENTRATION
- days 0, 12, and 19 (fresh solutions) and 2, 14, and 21 (before renewal or end).
- DOC deterimination
Test solutions
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dilution water: M4 medium (Elendt, 1990)
- Test item concentration in stock solution: 0.1 g/l
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS
- Strain: Daphnia magna, clone 5 - Source/supplier: Hüls AG (inhouse)
- Breeding method: In M4 medium (Elendt, 1990), water renewal each 2-3 days, isolation of offspring for further breeding each aaprox. 4 weeks
- Age: < 24 hours on day 0
- Feeding: Scenedesmus subspicatus, daily as much as consumed
- Pretreatment: Removal of adults 24 h prior to testing
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- - Test temperature: 21 +- 1 °C
- pH:
- 7.6-8.3 (1 determination per concentration on days 2, 5, 12, 14, 19, and 21)
- Dissolved oxygen:
- 91-103 % saturation (1 determination per concentration on days 2, 5, 12, 14, 19, and 21)
- Nominal and measured concentrations:
- Nominal/measured concentrations (DOC):
6 / 12 / 25 / 50 / 100 mg/l nominal
6 / 12 / 25 / 47 / 94 mg/l mean of measured (fresh solutions)
5 / 11 / 20 / 34 / 73 mg/l measured, day 2
5 / 10 / 21 / 42 / 75 mg/l measured, day 14
4 / 9 / 22 / 44 / 87 mg/l measured, day 21 - Details on test conditions:
- DILUTION WATER
- Synthetic freshwater according to Elendt (1990)
TEST CONDITIONS
- Feeding during test: daily with Scenedesmus subspicatus, days 0-6: 5.0E+06 cells/animal daily, days 7-21: 1.0E+07 cells/animal daily
- Control group: feeding as above, no test substance
- Photoperiod: 16 hours 1000 Lux / 8 hours 100 Lux
TEST SYSTEM
- Renewal of test solution: Each Monday, Wednesday, and Friday - Exposure vessel type: 150 ml beakers with approx. 80 ml test solution - Number of replicates, individuals per replicate: 10 replicates + 2 spare replicates (to substitute males if required) with 1 individual each
EFFECT PARAMETERS MEASURED
- mortality, number of juveniles (reproduction rate)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- The analytical values were close to nominal (within +- 20 % except for stability control at >= 50 mg/l). Thus the evaluation was based on nominal
concentrations.
Any other information on results incl. tables
- Effect data (Immobilisation and reproduction):
--------------------------------------------------------
Concentration Mortality Offspring/adult Inhibition
--------------------------------------------------------
0 mg/l 0 % 64 +- 10 0 %
6 mg/l 10 % 66 +- 10 -4 %
12 mg/l 0 % 67 +- 14 -6 %
25 mg/l 0 % 67 +- 9 -5 %
50 mg/l 0 % 39 +- 18 39 % *
100 mg/l 40 % 5 +- 4 93 % *
--------------------------------------------------------
* significant
- Other effects: The change in pH value from fresh to used test solution was
> 0.3 in one case (100 mg/l: 8.7 on day 12, 8.1 on day 14). This had no effect
on the result and thus on the validity of the study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The long-term effects of 3,3,5 -trimethylcyclohexanole on the reproduction rate and the mortality of Daphnia magna were investigated in a semi-static test over a period of 21 days by Hüls AG (1994). The study was conducted according to OECD 202, part II (1984). Five concentrations ranging from 6 to 100 mg/L (nominal concentrations) were tested. No effects on the reproduction rate of Daphnia magna were observed at 25 mg/L (NOEC) and below. The lowest concentration at which a reduction in reproduction occured was 50 mg/L (LOEC). Effects on the mortality of the parent animals was observed at 100 mg/L (40% mortality after 21 days).
NOEC (reproduction): 25 mg/L
LOEC (reproduction): 50 mg/L
The study was assessed as "reliable without restrictions".
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