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EC number: 268-596-7 | CAS number: 68130-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 08 - 23 Aug 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study period of 15 days does not meet the criteria of today standard methods recommending 21 days study duration. However the validity criteria stated in ECHA Guidance on information requirements and chemical safety assessment, Chapter R. were fulfilled (e.g. 80% survival in the control, surviving females produce three broods in 21 days or less, > 60 brood neonates per female in the control on average). Thus all requested endpoints could be determined (time to first brood, number of offspring produced per female, growth and survival) and NOELR values for survival or reproductive endpoints could be calculated. In accordance with ECHA Guidance on information requirements and chemical safety assessment, Chapter R.7b the study is considered as valid.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guideline 202 "Daphnia sp. Acute Immobilization Test and Reproduction Test", Part 2 (1993)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- Only 15 d test duration; Adopted in 1998
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: One set of final and initial WAF samples was collected on day 14 for chemical analysis. WAF samples (initial and final) of the control, the lowest (24 mg/L), middle (242 mg/L), and highest ( 2570 mg/L) were collected in duplicate.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Generation of WAFs was carried out using a modification of the procedure developed by Anderson et al. (1974). Six individual WAF setups, one for each of the five exposure concentrations and one for control were prepared. An appropriate amount of test material was added to 2000 mL of Mobil Technical Center well water in each corresponding WAF aspirator bottle. Bottles were capped with parafilm and stirred for 20 h at room temperature. After stirring, the phases were allowed to separate for 4 h. 800 mL of the water phase were subsequently drained from the bottom spout.
- Differential loading: yes
- Controls: Mobil Technical Center well water without test material. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: The primary culture originated from Aquatic Research Organisms, Hampton, NH. The in-house culture had been maintained in the laboratory since June 19, 1996.
- Age of parental stock: Daphnids used for the test were less than 24 h old. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 15 d
- Test temperature:
- 19.3-20.5 °C
- pH:
- 7.84-8.41
- Nominal and measured concentrations:
- Nominal: 0, 24, 97, 242, 1018 and 2570 mg/L
Measured: Below the detection limit of 0.03 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL polystyrene cups with 40 mL of test solution
- Type (delete if not applicable): covered with a plexiglass sheet
- Renewal rate of test solution (frequency/flow rate): every 48 h
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Mobil Technical Center well water
- Intervals of water quality measurement: at the beginning of the study and after every renewal period
OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h dark
EFFECT PARAMETERS MEASURED: immobilisation and reproduction - Reference substance (positive control):
- no
- Key result
- Duration:
- 15 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- immobilisation
- Key result
- Duration:
- 15 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- reproduction
- Key result
- Duration:
- 15 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: 0%
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 July to 15 August 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- see "Any other information on materials and methods incl. tables" for details.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- As above
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical sampling
Sampling Intervals and Solution Composition:
Days 0, 6, 14, and 20 (new): Each treatment level and control prior to division into replicate vessels
Days 2, 8, 16, and 21 (aged): Composite of all available replicate vessels within each treatment level and control
Number Of Samples Taken from Each Exposure Solution: One sample at each interval. In addition, subsamples of the test solutions were also collected at each sampling interval and stored frozen as archive samples.
Sampling Location: Approximate midpoint from the surface, bottom, and sides of the vessel
Sampling Device: Pipet
Quality Control (QC) Samples: Three samples per sampling interval, prepared in dilution water at nominal concentrations approximating the test concentration range
Method of Analysis: Gas chromatography with mass spectrometry detection (GC-MSD)
Average Recovery Range: LOQ (0.000200 mg/L): 82.5 ± 10.1% (RSD: 12.3%)
High (100 mg/L): 104 ± 5.34% (RSD: 5.12%)
Total: 93.4 ± 13.8% (RSD: 14.8%)
Medium Used: Fortified laboratory well water
QC Sample Limit of Acceptance: 70.0 to 120%
QC samples were prepared in fortified laboratory well water at each sampling interval at nominal test substance concentrations approximating the test concentration range and remained with the exposure solution samples throughout the analytical process. Analysis of the QC samples was used to judge the precision and quality control maintained during the analytical process. All exposure solutions and QC samples were analyzed for test substance concentration using methodology validated at Smithers Viscient (DeVellis, 2018). Conditions and procedures used throughout the analysis of exposure solutions and QC samples during this study were similar to those used in the method validation study. - Vehicle:
- no
- Details on test solutions:
- Representative samples of the dilution water source were analyzed periodically for the presence of pesticides, PCBs, and toxic metals by Eurofins Lancaster Laboratories Environmental, Lancaster, Pennsylvania (U.S. EPA, 1997). None of these compounds have been detected at concentrations that are considered toxic in any of the water samples analyzed, in agreement with ASTM (2007) standard practice. Daphnid cultures are maintained in water from the same source as the dilution water utilized in this study and have successfully survived and reproduced over several generations. The acceptable performance of the daphnid cultures, in combination with the previously mentioned analyses, confirmed the acceptability of this dilution water for use during the conduct of bioassays.
Test Concentrations
Selection of nominal concentrations for the chronic toxicity exposure with daphnids was based on the results of a 21-day preliminary exposure conducted under static-renewal conditions at Smithers Viscient and in consultation with the Study Sponsor.
Nominal Loading Rate Concentrations: 6.3, 13, 25, 50, and 100 mg/L (6.3, 13, 25, 50, and 100% of a 100 mg/L water accommodated fraction (WAF))
Co-Solvent: None
Co-Solvent Load: Not applicable
Solution Preparation
Primary Stock Concentration: 100 mg/L loading rate
Stock Vessel: Glass beaker
Stock Volume: 3.5 L
Diluent: Fortified laboratory well water
Amount of Test Substance Added (for example): 0.3495 g (tested as 100%)
The primary stock solution was prepared as a WAF by adding the aliquot of test substance directly to the total volume of dilution water in a glass beaker. The stock solution was observed to be clear and colorless with visible oily globules of the test substance floating on the surface of the solution. The solution was then covered with plastic wrap and mixed slowly with a Teflon coated stir bar and stir plate for a total mixing time of approximately 48 hours. Following mixing, the solution was observed to be clear and colorless with an oily globule of test substance on the surface of the solution. Approximately 2.5 L of the solution water-accommodated fraction (WAF) was then siphoned into a second 4.0-L glass beaker, avoiding the water surface. The resulting solution was observed to be clear and colorless with no visible undissolved test substance - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids were selected as the test organism since they are recommended by OECD and are commonly used in chronic freshwater invertebrate toxicity tests. The Daphnia magna used in this toxicity test were obtained from laboratory cultures maintained at Smithers Viscient. Daphnids were obtained for testing by removing all immature daphnids from the culture vessel, thus isolating sexually mature daphnids approximately 24 hours prior to exposure initiation. Young produced by these daphnids within 24 hours of isolation were used in the exposure. The culture water was prepared by fortifying well water based on the formula for hard water (U.S. EPA, 1975) and filtering it through an Amberlite XAD 7 resin column to remove any potential organic contaminants.
Information on test organism
Species: Daphnia magna
Source: Smithers Viscient culture
Age: <24 hours old at exposure initiation
Culture Vessel: 1.0-L glass beaker containing 0.80 L of water
Food Type: unicellular green algae, Ankistrodesmus falcatus (4 × 107 cells/mL) and a suspension of YCT (yeast, cereal leaves, and flaked fish food)
Feeding Frequency: Once daily during culture
Culture Conditions Two Weeks Prior to Testing
Water Type: Fortified laboratory well water
pH: 7.9 to 8.4
Photoperiod: 16 hours light: 8 hours dark
Light Intensity: 41 to 68 footcandles (440 to 730 lux)
Temperature (°C): 20 to 22
Dissolved Oxygen: 7.1 to 8.9 mg/L
Conductivity ): 780 to 840 µS/cm
Total hardness as CaCO3 (mg/L): 160 to 180
Total alkalinity as CaCO3 (mg/L): 80 to 86
Representative samples of the food source were periodically analyzed for the presence of pesticides, PCBs, and toxic metals by Eurofins Lancaster Laboratories Environmental, Lancaster, Pennsylvania (U.S. EPA, 1997). None of these compounds have been detected at concentrations considered toxic in any of the samples analyzed, in agreement with ASTM (2007) standard practice. Based on the analysis for pesticides, the food source was considered to be of acceptable quality since all analyte concentrations were below levels of concern. - Test type:
- static
- Water media type:
- other: Fortified laboratory well water
- Remarks:
- more information provided in "Details on test organisms"
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- The exposure was initiated when daphnids, <24 hours old, were impartially distributed to each of the ten replicates for each nominal concentration and control.
- Post exposure observation period:
- Not specified
- Hardness:
- As CaCO3 160 to 190 mg/L
- Test temperature:
- 20 to 22 °C
- pH:
- 7.9 to 8.4
- Dissolved oxygen:
- 7.1 to 8.9 mg/L
- Salinity:
- Not specified
- Conductivity:
- 800 to 870 µS/cm
- Nominal and measured concentrations:
- Nominal Loading Rate Concentrations: 6.3, 13, 25, 50, and 100 mg/L (6.3, 13, 25, 50, and 100% of a 100 mg/L water accommodated fraction (WAF))
- Details on test conditions:
- Temperature controlled by: Environmental chamber set to maintain temperature at 20 ± 2 °C
Duration: 21 days
Photoperiod: 16 hours light: 8 hours dark
Transition Period: 15- to 30-minute transition period was controlled with an automatic timer.
Light Intensity: 15 to 20 µE m-2 s-1 measured with a Licor Model LI 250A photometer
Bulb Type: Fluorescent bulbs
Food Type: Algal suspension (Ankistrodesmus falcatus; 4 x 107 cells/mL):
250 µL (test days 0 through 7),
300 µL (test days 8 through 15), and
350 µL (test days 16 through 21)
Yeast, cereal leaves, and digested flaked fish food (YCT) suspension: 50 µL
Equivalent to approximately 0.20 mg carbon/daphnid/day
Feeding Frequency: Once daily
Replication: Ten replicates per treatment level and the control, each labeled to identify the nominal test concentration and designated replicate (A through J)
Control of Bias: Impartial addition of daphnids
Aeration: None
Exposure Vessel Size/Material: 100-mL clear glass beakers
Exposure Vessel Solution Volume: 80 mL
Exposure Vessel Cleaning: Cleaned prior to initiation
Test Solution Renewal
At exposure initiation and at each renewal interval (every 48 hours throughout the 21-day exposure), fresh Hatcol 1510 solutions were prepared according to the method outlined. At each renewal period, freshly prepared test solution was added to a second set of 100-mL glass beakers until each vessel contained 80 mL of solution. On renewal days, daphnids were carefully transferred from the aged solution into the freshly prepared exposure solutions and the food suspensions were then added to the newly prepared solutions.
Biological Observations
The number of immobilized adult daphnids and observations of abnormal behavior were recorded daily. Immobilization is defined as the inability of daphnids to swim within 15 seconds of gentle agitation of the exposure vessel. Numbers of offspring were determined upon the first
brood release in any vessel and daily throughout the remainder of the test. Offspring were removed, counted, and discarded at each observation interval. In addition, the number of immobilized offspring and the time to first brood release were recorded for each treatment level
and control. Observations of physical characteristics of exposure solutions (e.g., precipitate, cloudy solution) were recorded whenever the test organisms were observed. At exposure termination (day 21), the total body length of each surviving adult daphnid was measured. Daphnids were measured (to the nearest 0.05 mm) from the apex of the head to the base of the shell spine using an Olympus SZH-ILLD-200 Stereoscope in combination with a calibrated ocular. - Reference substance (positive control):
- not required
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF concentrations
- Basis for effect:
- other: Survival, reproduction, length
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF concentrations
- Basis for effect:
- other: Survival, reproduction, length
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF concentrations
- Basis for effect:
- other: Survivial, reproduction, length
- Remarks on result:
- other: EC value was empirically estimated; therefore, corresponding 95% confidence interval could not be determined.
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.001 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Survival, reproduction, length
- Remarks on result:
- other: No effects at the limit of solubility in water
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Survival, reproduction, length
- Remarks on result:
- other: No effects at the limit of solubility in water
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Survival, reproduction, length
- Remarks on result:
- other: EC value was empirically estimated; therefore, corresponding 95% confidence interval could not be determined.
- Remarks:
- No effects at the limit of solubility in water
- Details on results:
- 21-Day Preliminary Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Mean Percent Survival, Reproduction, and Total Body Length of Parental Daphnids
Based on these results and in consultation with the Study Sponsor, nominal concentrations of 6.3, 13, 25, 50, and 100 mg/L (6.3, 13, 25, 50, and 100% of a 100 mg/L water accommodated fraction (WAF)), and a control were selected for the definitive static-renewal exposure.
Prior to the definitive exposure, two initial definitive exposures were conducted at Smithers Viscient between July 2017 and January 2018. The first exposure was repeated due to analytical recoveries below the limit of quantitation (LOQ). The second exposure was repeated due to aged solution recoveries below the LOQ
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Concentrations Measured in Exposure Solutions
Solution concentrations decreased substantially over the course of each renewal period. The cause of these losses is unknown, but is likely related to adsorption or degradation and can be reliably attributed to the behavior of the test substance under the exposure conditions due to its inherent chemical properties. There was some variability in the measured concentrations of the individual WAF preparations (the new solutions in the table above). This is likely related to the inherent variability associated with the generation of samples at the limit of functional solubility. Considerable precautions were taken to minimize this potential variability associated with the WAF preparation, including factors such as maintaining consistent mixing time and mixing speed.
Analysis of 21 of the 24 QC samples resulted in measured concentrations which were consistent with the predetermined recovery range and ranged from72.3 was 120%of the nominal fortified concentrations (0.000200, 0.00200, and 0.0200 mg/L). These results established that the appropriate precision and quality control was maintained during the analysis of the exposure solutions.
The recovery of one QC sample on day 0 was 160%, one QC on day 8 was 198%, and one QC on day 14 was 1210%, which are outside the established acceptance criterion (i.e., 70 to 120%). These results are likely due to fortification errors limited to these samples and not sample processing as the remaining QC sample results are well within the expected range and the recoveries of the exposure solutions maintain a consistent trend. Given the fact that the two remaining QC samples for each impacted interval are within the acceptable range and the likely cause of the failure is limited to the individual QC samples, the failure of these QC samples to meet acceptance criterion has minimal impact on the evaluation of test sample results.
Based on the results of this study, the 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 100 mg/L and >100 mg/L, respectively, based on nominal loading rate WAF concentrations.
The 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 0.00085 mg/L and >0.00085 mg/L, respectively, based on mean measured active ingredient concentrations. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not specified
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of this study, the 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 100 mg/L and >100 mg/L, respectively, based on nominal loading rate WAF concentrations.
The 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 0.00085 mg/L and >0.00085 mg/L, respectively, based on mean measured active ingredient concentrations, indicatting that there were no effects at the limit of solubility in water. - Executive summary:
The objective of this study was to evaluate the chronic effects of decanoic acid, mixed esters with heptanoic acid, octanoic acid and trimethylolpropane (Hatcol 1510) on the survival, reproduction, and growth (mean total body length) of the daphnid, Daphnia magna.
The exposure was performed under static-renewal conditions for a period of 21 days. Results of this study were used to determine the No-Observed-Effect Concentration (NOEC) and Lowest Observed-Effect Concentration (LOEC) for the most sensitive biological parameter monitored (e.g., daphnid survival, reproduction, and total body length). In addition, a median effective concentration (EC50), EC20, and EC10 values for survival, reproduction, and growth (total body length) were determined. The results of this study are based on nominal loading rate WAF and mean measured concentrations of Hatcol 1510
Based on the results of this study, the 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 100 mg/L and >100 mg/L, respectively, based on nominal loading rate WAF concentrations.
The 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 0.00085 mg/L and >0.00085 mg/L, respectively, based on mean measured concentrations , indicating that there were no effects at the limit of solubility in water.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reason / purpose for cross-reference:
- read-across source
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 15 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- immobilisation
- Remarks on result:
- other: CAS 11138-60-6
- Key result
- Duration:
- 15 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- reproduction
- Remarks on result:
- other: CAS 11138-60-6
- Key result
- Duration:
- 15 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 2 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- reproduction
- Remarks on result:
- other: CAS 11138-60-6
- Validity criteria fulfilled:
- not applicable
- Remarks:
- Read-across
- Conclusions:
- The substance, CAS 68130-53-0; EC 268-596-7, is analogous to the substances to be read across to, in terms of basic form, and the degree of substitution of functional groups is not considered to effect the proposed read across for the endpoint of long-term toxicity to aquatic invertebrates. The substance is not considered to be toxic to aquatic invertebrates following long term exposure on the basis of read across.
Referenceopen allclose all
No effects were observed in the range of water solubility with an NOELR = 2570 mg/L (15 d) based on reproduction and immobilisation.
Table 1: Final total reproduction counts taken during the static renewal three-brood chronic toxicity study of the WAF of the test substance to Daphnia magna
Replicates |
Control |
Exposure Concentrations (ppm) |
||||
24 |
97 |
242 |
1018 |
2570 |
||
A |
89 |
107 |
102 |
125 |
109 |
112 |
B |
94 |
113 |
111 |
101 |
135 |
127 |
C |
115 |
123 |
129 |
120 |
88 |
68 |
D |
108 |
92 |
92 |
125 |
130 |
109 |
E |
124 |
122 |
127 |
100 |
123 |
118 |
F |
117 |
120 |
116 |
121 |
125 |
127 |
G |
115 |
98 |
0 |
124 |
142 |
129 |
H |
89 |
79 |
102 |
103 |
128 |
129 |
I |
114 |
128 |
136 |
102 |
79 |
116 |
J |
128 |
151 |
140 |
56 |
130 |
116 |
Totals |
1093 |
1133 |
1055 |
1077 |
1189 |
1151 |
Average |
109 |
113 |
106 |
108 |
119 |
115 |
Std. Dev. |
14 |
20 |
40 |
21 |
21 |
18 |
21-Day Preliminary Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Mean Percent Survival, Reproduction, and Total Body Length of Parental Daphnids
Nominal Concentration |
Mean Percent Survival |
Mean (SD)aNumber of Offspring Released per Surviving Daphnid |
Mean (SD) Number of Offspring Released per Female |
Mean (SD)a Total Body Length (mm) |
Control |
100 |
70 (24) |
70 (24) |
4.28 (0.32) |
0.010 |
100 |
92 (11) |
92 (11) |
4.41 (0.10) |
0.10 |
100 |
92 (6) |
92 (6) |
4.58 (0.07) |
1.0 |
100 |
89 (28) |
89 (28) |
4.57 (0.08) |
10 |
100 |
104 (21) |
104 (21) |
4.64 (0.10) |
100 |
100 |
110 (46) |
110 (46) |
4.57 (0.13) |
a SD = Standard Deviation
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Water Quality Parameters Measured in the Exposure Solutions
Nominal Concentration (mg/L loading rate WAF) |
|
Ranges |
||||||
Dissolved Oxygen |
Temperature (°C)a |
pH |
Total Hardness (mg/L CaCO3) |
Total Alkalinity (mg/L CaCO3) |
Conductivity (µS/cm) |
|||
(mg/L) |
(% of Saturation) |
|||||||
|
|
|
|
|
|
|
|
|
Control |
New |
8.2 - 9.6 |
93 - 100 |
19 - 22 |
8.0 - 8.3 |
180 |
86 |
790 - 820 |
|
Aged |
8.4 - 11 |
94 - 120 |
19 - 21 |
7.9 - 8.8 |
190 |
88 |
860 |
|
|
|
|
|
|
|
|
|
6.3 |
New |
8.2 - 9.4 |
92 - 100 |
19 - 22 |
8.0 - 8.3 |
NAb |
NA |
NA |
|
Aged |
8.2 - 10 |
93 - 120 |
20 - 21 |
8.0 - 8.8 |
NA |
NA |
NA |
|
|
|
|
|
|
|
|
|
13 |
New |
8.2 - 9.4 |
92 - 100 |
19 - 21 |
8.1 - 8.3 |
NA |
NA |
NA |
|
Aged |
8.2 - 10 |
92 - 110 |
19 - 21 |
7.9 - 8.7 |
NA |
NA |
NA |
|
|
|
|
|
|
|
|
|
25 |
New |
8.2 - 9.3 |
92 - 100 |
19 - 21 |
8.1 - 8.3 |
NA |
NA |
NA |
|
Aged |
7.9 - 11 |
88 - 120 |
19 - 21 |
7.9 - 8.7 |
NA |
NA |
NA |
|
|
|
|
|
|
|
|
|
50 |
New |
8.2 - 9.4 |
92 - 100 |
19 - 22 |
8.1 - 8.3 |
NA |
NA |
NA |
|
Aged |
7.8 - 10.0 |
88 - 110 |
19 - 21 |
7.9 - 8.7 |
NA |
NA |
NA |
|
|
|
|
|
|
|
|
|
100 |
New |
8.3 - 9.5 |
92 - 100 |
19 - 22 |
8.1 - 8.3 |
180 - 200 |
88 |
760 - 860 |
|
Aged |
7.9 - 9.9 |
89 - 110 |
19 - 21 |
7.9 - 8.7 |
190 |
88 |
870 |
|
|
|
|
|
|
|
|
|
a Continuous temperature monitoring of the environmental chamber established a minimum/maximum temperature range of 19 to 20 °C during the exposure period.
b NA = Not Applicable; measurement not required by protocol.
NOTE: Values presented have been rounded to two significant figures.
These results established that the water quality conditions throughout the exposure remained within acceptable ranges for the survival, reproduction, and growth of Daphnia magna.
Analytical Results
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Concentrations Measured in Exposure Solutions
Nominal Concentration (mg/L) |
Measured Concentration (mg/L) |
||||||||
Day 0 (new) |
Day 2 (aged) |
Day 6 (new) |
Day 8 (aged) |
Day 14 (new) |
Day 16 (aged) |
Day 20 (new) |
Day 21 (aged) |
Mean (SD)a |
|
Control |
<0.000063b |
<0.000063 |
<0.000063 |
<0.000063 |
<0.000063 |
<0.000063 |
<0.000063 |
<0.000063 |
NAc |
|
|
|
|
|
|
|
|
|
|
6.3 |
<0.000063d |
<0.000063d |
0.000069 |
<0.000063d |
0.00016 |
<0.000063d |
0.000069 |
<0.000063d |
0.000057 (0.000045) |
|
|
|
|
|
|
|
|
|
|
13 |
<0.000063d |
<0.000063d |
<0.000063d |
<0.000063d |
0.0016 |
0.000084 |
0.00018 |
<0.000063d |
0.00025 (0.00055) |
|
|
|
|
|
|
|
|
|
|
25 |
<0.000063d |
<0.000063d |
0.00010 |
<0.000063d |
0.0031 |
0.00017 |
0.00023 |
<0.000063d |
0.00047 (0.0011) |
|
|
|
|
|
|
|
|
|
|
50 |
0.00036 |
<0.000063d |
0.00023 |
<0.000063d |
0.0033 |
0.00027 |
0.00043 |
0.00012 |
0.00060 (0.0011) |
|
|
|
|
|
|
|
|
|
|
100 |
0.00073 |
<0.000063d |
0.00044 |
<0.000063d |
0.0035 |
0.00055 |
0.0012 |
0.00022 |
0.00085 (0.0012) |
|
|
|
|
|
|
|
|
|
|
QCe#1 0.000200 |
0.000317 (160f) |
0.000173 (86.3) |
0.000234 (117) |
0.000395 (198f) |
0.000183 (91.4) |
0.000217 (109) |
0.000158 (78.9) |
0.000227 (113) |
|
|
|
|
|
|
|
|
|
||
QC #2 0.00200 |
0.00208 (104) |
0.00171 (85.5) |
0.00167 (83.3) |
0.00225 (113) |
0.00220 (110) |
0.00184 (91.8) |
0.00240 (120) |
0.00186 (93.0) |
|
|
|
|
|
|
|
|
|
||
QC #3 0.0200 |
0.0161 (80.5) |
0.0179 (89.7) |
0.0210 (105) |
0.0178 (88.9) |
0.243 (1210f) |
0.0145 (72.3) |
0.0189 (94.5) |
0.0155 (77.3) |
|
a Mean measured concentration and standard deviation (SD) values were calculated using actual (unrounded) analytical results and not with the rounded values presented in this table.
b Concentrations expressed as less than values were below the method detection limit (MDL). The MDL is dependent upon the lowest concentration calibration standard and the dilution factor of the controls.
c NA = Not Applicable
d As a conservative measure, a value of ½ MDL was used for these samples to generate a mean measured concentration. See below for additional discussion.
e QC = Quality Control sample; percent recovery is presented in parentheses.
f The percent recovery of this QC sample was outside of the acceptance criteria (i.e., 70.0 to 120%,).See below for additional discussion.
Biological Results
21-Day Chronic Exposure of Daphnids(Daphnia magna)to Hatcol 1510-Survival of Parental Daphnids and Number of Offspring Released per Female in the Control
Control |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
0 |
1 |
0 |
0 |
4 |
7 |
0 |
5 |
8 |
6 |
0 |
100 |
|
9 |
4 |
4 |
2 |
1 |
0 |
0 |
4 |
0 |
0 |
0 |
0 |
100 |
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/X |
0 |
1 |
90 |
|
11 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
6 |
X |
21 |
1 |
90 |
|
12 |
24 |
15 |
16 |
21 |
17/X |
20 |
24 |
2 |
X |
0 |
2 |
80 |
|
13 |
0 |
0 |
0 |
0 |
X |
0 |
0 |
0 |
X |
0 |
2 |
80 |
|
14 |
0 |
0 |
0 |
0 |
X |
0 |
0 |
22 |
X |
20 |
2 |
80 |
|
15 |
0 |
22 |
14 |
27 |
X |
34 |
27 |
0 |
X |
0 |
2 |
80 |
|
16 |
26 |
0 |
0 |
0 |
X |
0 |
0 |
0 |
X |
0 |
2 |
80 |
|
17 |
0 |
0 |
0 |
0 |
X |
0 |
0 |
40 |
X |
40 |
2 |
80 |
|
18 |
0 |
26 |
22 |
30 |
X |
34 |
38 |
0 |
X |
0 |
2 |
80 |
|
19 |
34 |
0 |
0 |
0 |
X |
0 |
0 |
0 |
X |
0 |
2 |
80 |
|
20 |
0 |
0 |
0 |
0 |
X |
0 |
0 |
38 |
X |
37 |
2 |
80 |
|
21 |
0 |
34 |
34 |
39 |
X |
42 |
43 |
0 |
X |
0 |
2 |
80 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
88 |
102 |
88 |
118 |
NA |
137 |
136 |
113 |
NA |
124 |
Mean (N): |
113 (8) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
88 |
102 |
88 |
118 |
21 |
137 |
136 |
113 |
8 |
124 |
Mean (N): |
94 (10) |
X = Immobilized daphnid
N = Number of daphnids evaluated
NA = Not Applicable
21-Day Chronic Exposure of Daphnids(Daphnia magna)to Hatcol 1510-Survival of Parental Daphnids and Number of Offspring Released per Female in the 6.3 mg/L Nominal Loading Rate WAF Treatment Level
6.3 mg/L nominal loading rate WAF |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
5 |
5 |
6 |
7 |
9 |
2 |
9 |
8 |
11 |
6 |
0 |
100 |
|
9 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
20 |
0 |
100 |
|
11 |
29 |
18 |
20 |
21 |
19 |
9 |
23 |
17 |
22 |
0 |
0 |
100 |
|
12 |
0 |
0 |
1 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
100 |
|
13 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
34 |
0 |
100 |
|
14 |
31 |
28 |
27 |
28 |
31 |
21 |
27 |
30 |
27 |
0 |
0 |
100 |
|
15 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
16 |
0 |
0 |
0 |
0 |
45 |
0 |
0 |
0 |
0/X |
45 |
1 |
90 |
|
17 |
55 |
42 |
46 |
43 |
3 |
4 |
44 |
44 |
X |
0 |
1 |
90 |
|
18 |
0 |
0 |
0 |
0 |
0 |
35 |
0 |
0 |
X |
0 |
1 |
90 |
|
19 |
0 |
0 |
48 |
0 |
41 |
0 |
0 |
0 |
X |
55 |
1 |
90 |
|
20 |
40 |
38 |
0 |
41 |
0 |
41 |
39 |
45 |
X |
0 |
1 |
90 |
|
21 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
X |
0 |
1 |
90 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
160 |
131 |
148 |
140 |
148 |
113 |
142 |
144 |
NA |
160 |
Mean (N): |
143 (9) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
160 |
131 |
148 |
140 |
148 |
113 |
142 |
144 |
60 |
160 |
Mean (N): |
135 (10) |
X = Immobilized daphnid
N = Number of daphnids evaluated
NA = Not Applicable
21-Day Chronic Exposure of Daphnids(Daphnia magna)to Hatcol 1510-Survival of Parental Daphnids and Number of Offspring Released per Female in the 13 mg/L Nominal Loading Rate WAF Treatment Level
13 mg/L nominal loading rate WAF |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
0 |
0 |
5 |
9 |
7 |
0 |
6 |
0 |
7 |
0 |
0 |
100 |
|
9 |
8 |
0 |
0 |
0 |
0 |
6 |
0 |
8 |
0 |
5 |
0 |
100 |
|
10 |
0 |
0 |
26 |
19 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
11 |
0 |
23 |
0 |
0 |
25 |
0 |
0 |
0 |
27 |
0 |
0 |
100 |
|
12 |
26 |
0 |
0 |
0 |
0 |
27 |
16 |
22 |
0 |
27 |
0 |
100 |
|
13 |
0 |
0 |
36 |
32 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
14 |
0 |
31 |
0 |
0 |
34 |
0 |
0 |
0 |
34 |
0 |
0 |
100 |
|
15 |
34 |
0 |
0 |
0 |
0 |
40 |
32 |
43 |
0 |
43 |
0 |
100 |
|
16 |
0 |
0 |
50 |
48 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
100 |
|
17 |
0 |
42 |
0 |
0 |
52 |
0 |
0 |
0 |
48 |
0 |
0 |
100 |
|
18 |
43 |
0 |
0 |
0 |
0 |
41 |
33 |
44 |
0 |
41 |
0 |
100 |
|
19 |
0 |
0 |
56 |
55 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
20 |
0 |
42 |
0 |
0 |
40 |
0 |
0 |
0 |
46 |
0 |
0 |
100 |
|
21 |
46 |
0 |
0 |
0 |
0 |
0 |
34 |
48 |
0 |
44 |
0 |
100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
157 |
138 |
173 |
163 |
158 |
115 |
121 |
165 |
162 |
160 |
Mean (N): |
151 (10) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
157 |
138 |
173 |
163 |
158 |
115 |
121 |
165 |
162 |
160 |
Mean (N): |
151 (10) |
N = Number of daphnids evaluated
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Survival of Parental Daphnids and Number of Offspring Released per Female in the 25 mg/L Nominal Loading Rate WAF Treatment Level
25 mg/L nominal loading rate WAF |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
9 |
6 |
7 |
4 |
4 |
8 |
5 |
7 |
9 |
1 |
10 |
0 |
100 |
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
11 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
12 |
31 |
30 |
26 |
25 |
38 |
27 |
30 |
29 |
24 |
40 |
0 |
100 |
|
13 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
14 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
15 |
42 |
41 |
43 |
34 |
42 |
0 |
40 |
37 |
22 |
52 |
0 |
100 |
|
16 |
0 |
0 |
0 |
0 |
0 |
33 |
27 |
0 |
0 |
0 |
0 |
100 |
|
17 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
18 |
41 |
39 |
42 |
40 |
46 |
47 |
35 |
41 |
38 |
50 |
0 |
100 |
|
19 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
0 |
100 |
|
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
21 |
48 |
46 |
50 |
41 |
46 |
43 |
40 |
46 |
50 |
53 |
0 |
100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
168 |
163 |
165 |
144 |
180 |
157 |
179 |
162 |
135 |
205 |
Mean (N): |
166 (10) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
168 |
163 |
165 |
144 |
180 |
157 |
179 |
162 |
135 |
205 |
Mean (N): |
166 (10) |
N = Number of daphnids evaluated
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 - Survival of Parental Daphnids and Number of Offspring Released per Female in the 50 mg/L Nominal Loading Rate WAF Treatment Level
50 mg/L nominal loading rate WAF |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
0 |
12 |
0 |
0 |
0 |
10 |
5 |
10 |
9 |
8 |
0 |
100 |
|
9 |
11 |
0 |
5 |
10 |
7 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
22 |
0 |
0 |
0 |
100 |
|
11 |
6 |
25 |
0 |
0 |
0 |
19 |
27 |
0 |
25 |
21 |
0 |
100 |
|
12 |
24 |
0 |
29 |
32 |
25 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
13 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
28 |
0 |
0 |
0 |
100 |
|
14 |
38 |
33 |
26 |
16 |
0 |
32 |
39 |
0 |
33 |
33 |
0 |
100 |
|
15 |
0 |
0 |
11 |
22 |
43 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
16 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
46 |
0 |
0 |
0 |
100 |
|
17 |
49 |
50 |
47 |
0 |
0 |
45 |
53 |
0 |
48 |
41 |
0 |
100 |
|
18 |
0 |
0 |
0 |
48 |
44 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
19 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
50 |
0 |
0 |
0 |
100 |
|
20 |
48 |
50 |
51 |
0 |
0 |
48 |
52 |
0 |
51 |
49 |
0 |
100 |
|
21 |
0 |
0 |
0 |
48 |
45 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
176 |
170 |
169 |
176 |
164 |
154 |
176 |
156 |
166 |
152 |
Mean (N): |
166 (10) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
176 |
170 |
169 |
176 |
164 |
154 |
176 |
156 |
166 |
152 |
Mean (N): |
166 (10) |
N = Number of daphnids evaluated
21-Day Chronic Exposure of Daphnids(Daphnia magna)to Hatcol 1510-Survival of Parental Daphnids and Number of Offspring Released per Female in the 100 mg/L Nominal Loading Rate WAF Treatment Level
100 mg/L nominal loading rate WAF |
|||||||||||||
Test Day |
Number of Live Offspring Released per Replicate Exposure Vessel |
Number of Adult Daphnids Immobilized |
Mean Percent Survival |
||||||||||
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
|
8 |
11 |
13 |
11 |
12 |
11 |
12 |
12 |
10 |
10 |
6 |
0 |
100 |
|
9 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
6 |
0 |
100 |
|
10 |
0 |
0 |
0 |
30 |
0 |
31 |
25 |
0 |
0 |
0 |
0 |
100 |
|
11 |
32 |
30 |
27 |
0 |
25 |
0 |
0 |
30 |
30 |
0 |
0 |
100 |
|
12 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
26 |
0 |
100 |
|
13 |
0 |
0 |
0 |
46 |
0 |
41 |
33 |
0 |
0 |
0 |
0 |
100 |
|
14 |
44 |
38 |
37 |
0 |
33 |
0 |
0 |
37 |
40 |
32 |
0 |
100 |
|
15 |
0 |
0 |
0 |
0/X |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
90 |
|
16 |
0 |
51 |
51 |
X |
0 |
57 |
49 |
0 |
0 |
0 |
1 |
90 |
|
17 |
54 |
0 |
0 |
X |
48 |
0 |
0 |
58 |
54 |
45 |
1 |
90 |
|
18 |
0 |
0 |
0 |
X |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
90 |
|
19 |
0 |
52 |
52 |
X |
0 |
59 |
56 |
0 |
0 |
0 |
1 |
90 |
|
20 |
54 |
0 |
0 |
X |
52 |
0 |
0 |
55 |
48 |
53 |
1 |
90 |
|
21 |
0 |
0/P |
0 |
X |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
90 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Total Number of Offspring Released per Surviving Female: |
|
|
|||||||||||
|
195 |
184 |
178 |
NA |
169 |
200 |
175 |
190 |
182 |
168 |
Mean (N): |
182 (9) |
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Total Number of Offspring Released per Female: |
|
|
|||||||||||
|
195 |
184 |
178 |
88 |
169 |
200 |
175 |
190 |
182 |
168 |
Mean (N): |
173 (10) |
X = Immobilized daphnid
N = Number of daphnids evaluated
NA = Not Applicable
As demonstrated by the performance of the control, the exposure system provided conditions that were appropriate for promoting acceptable survival and reproduction of the test species and met the minimum standard criteria established by OECD guidelines (i.e.,³80% survival and³60 offspring per female). Time of the first brood release was within normal performance expectation for this species.
21-DayChronicExposure of Daphnids(Daphnia magna)to Hatcol 1510-Mean Body Length at Termination
Nominal Concentration (mg/L loading rate WAF) |
Length (mm) |
|||||
Control |
6.3 |
13 |
25 |
50 |
100 |
|
Replicate |
|
|
|
|
|
|
A |
4.45 |
4.75 |
4.55 |
4.50 |
4.80 |
4.75 |
B |
4.30 |
4.55 |
4.65 |
4.55 |
4.70 |
4.60 |
C |
4.50 |
4.60 |
4.70 |
4.60 |
4.75 |
4.75 |
D |
4.10 |
4.60 |
4.65 |
4.60 |
4.75 |
NA |
E |
NA |
4.30 |
4.70 |
4.75 |
4.70 |
4.75 |
F |
4.45 |
4.45 |
4.50 |
4.70 |
4.70 |
4.70 |
G |
4.35 |
4.30 |
4.50 |
4.60 |
4.70 |
4.75 |
H |
4.50 |
4.50 |
4.70 |
4.60 |
4.65 |
4.85 |
I |
NA |
NA |
4.75 |
4.60 |
4.70 |
4.90 |
J |
4.60 |
4.70 |
4.70 |
4.70 |
4.65 |
4.75 |
Mean |
4.41 |
4.53 |
4.64 |
4.62 |
4.71 |
4.76 |
SDa |
0.15 |
0.16 |
0.091 |
0.075 |
0.046 |
0.085 |
a SD = Standard Deviation
NA = Not Applicable
21-Day Chronic Exposure of Daphnids (Daphnia magna) to Hatcol 1510 –Nominal Loading Rate WAF Concentrations Tested, Mean Percent Survival, Number of Offspring per Surviving Female, Number of Offspring per Female, and Total Body Length of Parental Daphnids
Nominal Concentration |
Mean Percent Survival |
Mean (SDa) Number of Offspring Released per Surviving Female |
Mean (SD) Number of Offspring Released per Female |
Mean (SD) Total Body Length (mm) |
|
|
|
|
|
Control |
80 |
113b(19) |
94 (45) |
4.41 (0.15) |
6.3 |
90 |
143 (15) |
135 (30) |
4.53 (0.16) |
13 |
100 |
151 (20) |
151 (20) |
4.64 (0.091) |
25 |
100 |
166 (20) |
166 (20) |
4.62 (0.075) |
50 |
100 |
166 (9.2) |
166 (9.2) |
4.71 (0.046) |
100 |
90 |
182 (11) |
173 (32) |
4.76 (0.085) |
|
|
|
|
|
a SD = Standard Deviation
b Coefficient of variation was 17%.
21-Day Chronic Exposure of Daphnids(Daphnia magna)to Hatcol 1510-Endpoint Summary
Results |
|||
|
Endpoints (mg/L, based on nominal loading rate WAF concentrations) |
||
Survival |
Reproduction (Total Living Offspring per Surviving Female) |
Length |
|
|
|
|
|
NOEC |
100 |
100 |
100 |
|
|
|
|
LOEC |
>100 |
>100 |
>100 |
|
|
|
|
EC10 (95% CI) |
>100 (NAa) |
>100 (NA) |
>100 (NA) |
|
|
|
|
EC20 (95% CI) |
>100 (NA) |
>100 (NA) |
>100 (NA) |
|
|
|
|
EC50 (95% CI) |
>100 (NA) |
>100 (NA) |
>100 (NA) |
|
|
|
|
a NA = Not Applicable. EC value was empirically estimated; therefore, corresponding 95% confidence interval could not be determined.
CI = Confidence Interval
NOEC = No-Observed-Effect Concentration
LOEC = Lowest-Observed-Effect Concentration
Results |
|||
|
Endpoints (mg/L, based on mean measured concentrations) |
||
Survival |
Reproduction (Total Living Offspring per Surviving Female) |
Length |
|
|
|
|
|
NOEC |
0.00085 |
0.00085 |
0.00085 |
|
|
|
|
LOEC |
>0.00085 |
>0.00085 |
>0.00085 |
|
|
|
|
EC10 (95% CI) |
>0.00085 (NAa) |
>0.00085 (NA) |
>0.00085 (NA) |
|
|
|
|
EC20 (95% CI) |
>0.00085 (NA) |
>0.00085 (NA) |
>0.00085 (NA) |
|
|
|
|
EC50 (95% CI) |
>0.00085 (NA) |
>0.00085 (NA) |
>0.00085 (NA) |
|
|
|
|
a NA = Not Applicable. EC value was empirically estimated; therefore, corresponding 95% confidence interval could not be determined.
CI = Confidence Interval
NOEC = No-Observed-Effect Concentration
LOEC = Lowest-Observed-Effect Concentration
ACCEPTABILITY CRITERIA
The following criteria are required for a test to be acceptable:
Acceptability Criteria |
Study Results |
Criterion Met (Yes/No) |
Immobilization of parental daphnids in the control will be≤20%. |
Immobilization of parental daphnids in the control was 20% |
Yes |
Mean number of live offspring produced per parental daphnid in the control will be≥60. |
Mean number of live offspring produced per surviving parental daphnid in the control was 113 |
Yes |
Coefficient of variation around the mean number of living offspring produced per parent animal in the control should be≤25%. |
Coefficient of variation around the mean number of living offspring produced per surviving parent daphnid in the control was17% |
Yes |
Ephippia should not be present. |
Ephippia were not observed. |
Yes |
Description of key information
21 day reproduction study using the test substance revealed that no toxicity was observed in daphnia magna above the limit of solubility.
A 15-day reproduction test with read-across substance, decanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol octanoate, showed no effects in the range of water solubility with an NOELR = 2570 mg/L (15 d) based on reproduction and immobilisation. Therefore, by read-across, the test substance is not expected to cause long-term toxicity in aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Decanoic acid, mixed esters with heptanoic acid, octanoic acid, and trimethylolpropane - Full Life-Cycle Toxicity Test with Water Fleas, Daphnia magna, Under Static-Renewal Conditions
The objective of this study was to evaluate the chronic effects of decanoic acid, mixed esters with heptanoic acid, octanoic acid and trimethylolpropane (Hatcol 1510) on the survival, reproduction, and growth (mean total body length) of the daphnid, Daphnia magna.
The exposure was performed under static-renewal conditions for a period of 21 days. Results of this study were used to determine the No-Observed-Effect Concentration (NOEC) and Lowest Observed-Effect Concentration (LOEC) for the most sensitive biological parameter monitored (e.g., daphnid survival, reproduction, and total body length). In addition, a median effective concentration (EC50), EC20, and EC10 values for survival, reproduction, and growth (total body length) were determined. The results of this study are based on nominal loading rate WAF and mean measured concentrations of Hatcol 1510
Based on the results of this study, the 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 100 mg/L and >100 mg/L, respectively, based on nominal loading rate WAF concentrations.
The 21 day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for all endpoints were determined to be 0.00085 mg/L and >0.00085 mg/L, respectively, based on mean measured concentrations , indicating that there were no effects at the limit of solubility in water.
Read across
A 15-day reproduction test with read-across substance, decanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol octanoate, showed no effects in the range of water solubility with an NOELR = 2570 mg/L (15 d) based on reproduction and immobilisation. The study period of 15 days does not meet the criteria of current recommendations regarding a 21 day study duration. However the validity criteria stated in ECHA Guidance on information requirements and chemical safety assessment, Chapter R.7b were fulfilled (e.g. 80% survival in the control, surviving females produce three broods in 21 days or less, > 60 brood neonates per female in the control on average). Thus all requested endpoints could be determined (time to first brood, number of offspring produced per female, growth and survival) and NOELR values for survival or reproductive endpoints could be calculated. In accordance with ECHA Guidance on information requirements and chemical safety assessment, Chapter R.7b the study is considered as valid.
A report justifying the read-across approach is included in IUCLID Chapter 13.
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