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EC number: 418-780-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline Study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Salmonella typhimurium strains
TA 98 hisD3052 rfa uvrB +R,
TA 100 hisG46 rfa uvrB +R,
TA 1535 hisG46 rfa uvrB,
TA 1537 hisC3076 rfa uvrB - Metabolic activation system:
- S9 Mix
- Test concentrations with justification for top dose:
- Concentration range in the main test (with metabolic activation): 4, 20, 100, 500, 2500, 5000 µg/plate
Concentration range in the main test (without metabolic activation): 4, 20, 100, 500, 2500, 5000 µg/plate - Vehicle / solvent:
- dissolved in double-distilled water at appropriate concentrations immediately before use
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- The test compound was dissolved in double-distilled water and a stock solution of
50 mg/ml was prepared for the highest concentration, which provided a final
concentration of 5000 µg/plate. Further dilutions of 2500, 500, 100, 20 and 4 µg/plate
were used in all experiments.
The test compound did not precipitate on the plates up to the highest investigated dose
of 5000 ug/plate.
The test compound proved to be not toxic to the bacterial strains.
A toxicity test using histidine-enriched agar plates and a dilution of the tester strain
TA 100 (designated TA 100 D) was performed in parallel with the second experiment.
The test compound proved to be not toxic to the bacterial strain. - Species / strain:
- other: as specified above
- Metabolic activation:
- with
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (> 5000 µg/plate)
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: as specified above
- Metabolic activation:
- without
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (> 5000 µg/plate)
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The strain TA 1535 showed an increased number of revertants
at concentrations 4 and 100 µg/plate. This effects was not
reproducible in a second test nor in a further one with TA1535. - Remarks on result:
- other: other: preliminary test
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
The results lead to the conclusion that Beta W 7 A 1.0 is not mutagenic in these
bacterial test systems either in the absence or in the presence of an exogenous
metabolizing system. - Executive summary:
Beta W 7 A 1.0 was tested for mutagenicity with the strains TA 100, TA 1535, TA 1537
and TA 98 of Salmonella typhimurium.
Two independent mutagenicity studies were conducted, each in the absence and in the
presence of a metabolizing system derived from a rat liver homogenate.
Additionally a third assay was performed with the strain TA 1535 in the presence of
S9-mix.
For all studies, the compound was dissolved in double-distilled water, and each
bacterial strain was exposed to 6 dose levels.
Doses for all studies ranged from 4 to 5000 ug/plate.
Control plates without mutagen showed that the number of spontaneous revertant
colonies was within the laboratory's historical control range and similar to that
described in the literature. All the positive control compounds showed the expected
increase in the number of revertant colonies.
Toxicity: In the mutagenicity experiments toxicity was not observed.
A toxicity test using histidine-enriched agar plates and a dilution of the tester strain
TA 100 (designated TA 100 D) was performed in parallel with the second experiment.
The test compound proved to be not toxic to the bacterial strain.
Mutagenicity: In the absence and in the presence of the metabolic activation system
Beta W 7 A 1.0 did not result in relevant increases in the number of revertants in any
of the bacterial strains.
Summarizing, it can be stated that Beta W 7 A 1.0 is not mutagenic in these bacterial
test systems either with or without exogenous metabolic activation at the dose levels
investigated.
Reference
The 59 fraction was prepared by the department conducting the study according to
Ames et. al (1975). Male 5prague Dawley rats (200-300 g), supplied by Harlan
Winkelmann, Gartenstrasse 27,33178 Borchen, Germany, received a single
intraperitoneal injection of Aroclor 1254 (500 mglkg body weight) 5 days before killing.
The livers were removed from at least 5-6 animals at approx. 0 to 4 °C using cold
sterile solutions and glassware, and were then pooled and washed in approx. 150 mM
KCI (approximately 1 ml/g wet liver). The washed livers were cut into small pieces and
homogenized in three volumes of KCl. The homogenate was centrifuged at approx.
9000 g for 10 minutes. The supernatant was the 59 fraction. This was divided into
small portions, rapidly frozen and stored at approx. - 80°C for not longer than six
months. The protein content was determined for every batch. Also for every batch of
59 an independent validation was performed with a minimum of two different
mutagens, e.g., 2-aminoanthracene and dimethylbenzanthracene to confirm metabolic
activation by microsomal enzymes.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
The results lead to the conclusion that Beta W 7 A 1.0 is not mutagenic in these
bacterial test systems either in the absence or in the presence of an exogenous
metabolizing system.
Justification for selection of genetic toxicity endpoint
OECD Guideline Study
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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