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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from a secondary source.
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 92/69/EEC, C.3
- Principles of method if other than guideline:
- Toxicity to algae study was carried out for time period of 72 hrs.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l
- Sampling method: 1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 +/- 2 deg C
- pH:
- 6.9-7.6 (beginning of test); 8.3-8.9 (end of test)
- Nominal and measured concentrations:
- concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Aeration: Yes
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: Yes
- Light intensity and quality: 8000 lux, white - Reference substance (positive control):
- not specified
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 420 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 91 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 255 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. And based on growth rate EC10 an EC50 values were 190 and > 420 mg/l.
- Executive summary:
Toxicity to algae study was carried out for time period of 72 hrs by following the method of Directive 92/69/EEC, C.3. Study was performed under static condition at temperature 24 +/- 2 deg C. Scenedesmus subspicatus was used as test organism. Test medium used was as described in guideline 92/69/EEC C.3. (1992). Stock solution was prepared as1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. Green algae were exposed at concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l. Light intensity used was 8000 lux, white. pH at beginning of test 6.9-7.6 and end of test was 8.3-8.9 respectively. sterile-aerated Erlenmeyer flasks was used as test vessel. The analyses were carried out by measurement of dissolved organic carbon. The DOC content was determined with a Shinadzu TOC Analyzer 500. Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. Based on growth rate EC10 an EC50 values were 190 and > 420 mg/l. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from a secondary source.
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 92/69/EEC, C.3
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. This solution serves as the primary dilution of the test substance. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- 24°C
- Nominal and measured concentrations:
- Nominal concentrations in mg/L in Main test 2: 0, 33, 67, 133, 278, 556, and 1000 mg/L.
Nominal concentrations in mg/L in Main test 4: 1.3, 2.5, 5.1, and 10.1. - Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: <1mg/L - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 321 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Other details not known
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 603 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on toxicity to growth rate of test alage, the 72 hrs NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively.
- Executive summary:
Toxicity to algae study was carried out for time period of 72 hrs by following the guideline OECD 201, Directive 92/69/EEC, C.3. Study was performed unser static condition. Scenedesmus subspicatus was used as test organism. Test was carried out at temperature of 24°C of TOC <1mg/L. Verification was done by using TOC-500 Infrared Analyzer. Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. Based on effect of growwth rate of test algae NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence considered to be not classified as per CLP classification criteria.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- calculation (if not (Q)SAR)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from computational model developed by USEPA
- Qualifier:
- according to guideline
- Guideline:
- other: Modeling database
- Principles of method if other than guideline:
- Estimation 96 hrs EC50 value of test chemical on aquatic algae by EPI Suite ECOSAR version 1.11.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- other: green algae
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Estimated data
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: EC50 value was determined to be > 100 mg/l (i.e 95897 mg/l)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l)
- Executive summary:
The toxicity of the test chemical to green algae was predicted using EPI Suite ECOSAR version 1.11. On the basis of effect of test chemical observed in a static system on the growth rate of the test organism during the 96 hr exposure duration, the median effect concentration (EC50) for the test chemical was estimated to be >100 mg/l (i.e 95897 mg/l). Thus, based on the EC50 value, test chemical can be considered as non-toxic to aquatic algae and hence, considered to be 'not classified' as per the CLP classification criteria.
Referenceopen allclose all
Description of key information
Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
Predicted data of target chemical and supporting weight of evidence studies for it's structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on algae. The studies are as mentioned below:
First study includes the toxicity of the test chemical to green algae was predicted using EPI Suite ECOSAR version 1.11. On the basis of effect of test chemical observed in a static system on the growth rate of the test organism during the 96 hr exposure duration, the median effect concentration (EC50) for the test chemical was estimated to be >100 mg/l (i.e 95897 mg/l). Thus, based on the EC50 value, test chemical can be considered as non-toxic to aquatic algae and hence, considered to be 'not classified' as per the CLP classification criteria.
Seond study includes toxicity to algae study was carried out for time period of 72 hrs by following the guideline OECD 201. Study was performed unser static condition. Scenedesmus subspicatus was used as test organism. Test was carried out at temperature of 24°C of TOC <1mg/L. Verification was done by using TOC-500 Infrared Analyzer. Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. Based on effect of growwth rate of test algae NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence considered to be not classified as per CLP classification criteria.
Third study includes toxicity to algae study was carried out for time period of 72 hrs by following the method of Directive 92/69/EEC, C.3. Study was performed under static condition at temperature 24 +/- 2 deg C. Scenedesmus subspicatus was used as test organism. Test medium used was as described in guideline 92/69/EEC C.3. (1992). Stock solution was prepared as1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. Green algae were exposed at concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l. Light intensity used was 8000 lux, white. pH at beginning of test 6.9-7.6 and end of test was 8.3-8.9 respectively. sterile-aerated Erlenmeyer flasks was used as test vessel. The analyses were carried out by measurement of dissolved organic carbon. The DOC content was determined with a Shinadzu TOC Analyzer 500. Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.
Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l).
Based on above information test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.
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