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Diss Factsheets
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EC number: 220-284-1 | CAS number: 2700-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 30 Mar 2021 to 01 Apr 2021
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N-bis(1-methylethyl)formamide
- EC Number:
- 220-284-1
- EC Name:
- N,N-bis(1-methylethyl)formamide
- Cas Number:
- 2700-30-3
- Molecular formula:
- C7H15NO
- IUPAC Name:
- N,N-Diisopropylformamide
Constituent 1
- Specific details on test material used for the study:
- Batch number: M-O303190723301F
Purity: 99.68%
Test animals / tissue source
- Species:
- cattle
- Details on test animals or tissues and environmental conditions:
- SOURCE OF COLLECTED EYES
- Source: Bovine eyes (from cattle at least six months old) were obtained from a local abattoir by Spear Products (Coopersburg, PA) and transported to MB Research in Hanks’ Balanced Salt Solution (HBSS) with penicillin-streptomycin on ice, in an insulated cooler, on 01 Apr 2021.
- Selection and preparation of corneas: The eyes were examined after receipt from the abattoir. Any cornea with gross abnormalities (e.g., neovascularization, pigmentation, opacity or scratches) was discarded.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- 0.75 ml
- Duration of treatment / exposure:
- 10 minutes
- Duration of post- treatment incubation (in vitro):
- 2 hours
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- NUMBER OF REPLICATES: 3
NEGATIVE CONTROL USED: MEM
POSITIVE CONTROL USED: 100% Ethanol
TREATMENT METHOD: closed chamber
POST-INCUBATION PERIOD: yes
REMOVAL OF TEST SUBSTANCE
- Number of washing steps after exposure period: After 10 (± 1) minutes, the test and control
articles were removed from the epithelium of the corneas and anterior chamber of the holder by washing with MEM containing phenol red. A final rinse was made with MEM without phenol red. The anterior and posterior chambers of the holders were then refilled with fresh MEM.
- POST-EXPOSURE INCUBATION: All corneas were incubated at 32 (± 1)°C for an additional two hours, MEM was then removed and replenished in each cornea holder.
METHODS FOR MEASURED ENDPOINTS:
- Corneal opacity: The opacity of each cornea was measured by comparing the treated cornea to the blank supplied with the opacitometer.
- Corneal permeability: passage of sodium fluorescein dye measured with the aid of Spectronic 20-D Colorimeter Spectrophotometer (OD490)
SCORING SYSTEM: In Vitro Irritancy Score (IVIS)
DECISION CRITERIA: the decision criteria as indicated in the TG was used.
Results and discussion
In vitro
Results
- Irritation parameter:
- in vitro irritation score
- Value:
- 58.08
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- The test substance is eye corrosive.
- Executive summary:
The Bovine Corneal Opacity and Permeability Test is performed on the test substanc. The IVIS value is 58.08, the test substance is eye corrosive.
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