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EC number: 418-240-3 | CAS number: 74401-04-0 N-METHYL-4-(P-FORMYLSTYRYL)-PYRIDINIUM METHYLSULFATE; SBQ; SBQ-SALZ
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- other: Body responsible for the test
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- A Guinea pig maximisation test was available
Test material
- Reference substance name:
- N-methyl-4-(p-formylstyryl)pyridinium methylsulfate
- EC Number:
- 418-240-3
- EC Name:
- N-methyl-4-(p-formylstyryl)pyridinium methylsulfate
- Cas Number:
- 74401-04-0
- Molecular formula:
- C16H17NO5S
- IUPAC Name:
- 4-[2-(4-formylphenyl)ethenyl]-1-methylpyridin-1-ium methyl sulfate
- Test material form:
- solid: particulate/powder
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Dunkin-Hartley
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- water
- Remarks:
- Distilled water
- Concentration / amount:
- 1% w/v
- Route:
- intradermal
- Vehicle:
- other: Mixture of Freund's Complete Adjuvant plus distilled water (1:1)
- Concentration / amount:
- 1% w/v
- Route:
- other: epicutaneous
- Vehicle:
- water
- Remarks:
- distilled
- Concentration / amount:
- 75% w/w
Challenge
- No.:
- #1
- Vehicle:
- water
- Remarks:
- distilled water
- Concentration / amount:
- a) 75% (w/w)
b) 50% (w/w)
- No. of animals per dose:
- Number of animals in test group: 10
Number of animals in negative control group: 5 - Positive control substance(s):
- no
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 75 %
- No. with + reactions:
- 8
- Total no. in group:
- 10
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 50 %
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 75 %
- No. with + reactions:
- 5
- Total no. in group:
- 10
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 50 %
- No. with + reactions:
- 5
- Total no. in group:
- 10
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 75 %
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 50 %
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 75 %
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 50 %
- No. with + reactions:
- 0
- Total no. in group:
- 5
Any other information on results incl. tables
Maximum concentration not causing irritating effects in preliminary test: 10 %
Signs of irritation during induction:
Intradermal Induction: well-defined erythema observed in all animals during induction. No adverse effects were noted in the control group.
Topical Induction: yellow coloured staining was noted at the induction sites of all test group animals at the 1 and 24-hour observations.The staining did not affect evaluation of skin responses. Very slight to well-defined erythema observed in all animals during induction. No adverse effects were noted in the control group.
Evidence of sensitisation of each challenge concentration: 9/10.
Other observations:
Yellow-coloured staining was commonly noted at the challenge sites of test and control group animals at the 24 and 48-hour observations. The staining did not affect evaluation of skin responses.
75% w/w in Distilled water: Positive skin responses (erythema grades 1-2) were seen in 8 test animals at 24 hours (including an incident of very slight oedema in one animal) and in 5 test animals at 48 hours. Desquamation was noted at the challenge site of one test animal at 48 hours.
50% w/w in Distilled water: Positive skin responses (erythema grade 1-2) were seen in 9 test animals at 24 hours (including an incident of very slight oedema in one animal) and in 5 test animals at 48 hours. Desquamation was noted at the challenge site of two test animals at 48 hours.
No skin reaction were seen in any control animals at either challenge concentration.
Applicant's summary and conclusion
- Interpretation of results:
- other: The test substance is classified as sub-category 1A skin sensitiser according to the CLP Regulation EC No. 1272/2008.
- Conclusions:
- > 60 % of the animals showed a positive response upon an intradermal induction with the test item at 1 % w/v.
- Executive summary:
The skin sensitisation potential of the test substance was evaluatedin vivoin a maximisation test on Guinea pigs according to the EU Method B.6 (92/69/EEC) and in compliance with GLP.
More than 60 % of the animals showed a positive response upon an intradermal induction with the test item at 1 % w/v.
The susbtance is considered as a skin sensitiser
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