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EC number: 683-326-1 | CAS number: 68815-17-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-04-24 to 2009-04-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: 100 mg/L
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Pretreatment: The activated sludge was washed three times with tap water and filtered using paper filter.
- Initial biomass concentration: The recommended amount of wet sludge for test vessels was calculated to obtain a suspended solids level of 4 g/L (±10%) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 19.9 - 20.9 °C
- Dissolved oxygen:
- The preparations (control 1, 1 test loading rate of the test item, reference item in 3 test concentrations, control 2) were made up in 15 minute intervals and were aerated continuously so that a dissolved oxygen level of ≥ 6.5 mg/L was achieved during the whole test duration.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Control 1, 1 test loading rate of test item, reference item in 3 test concentrations, control 2.
- Type (delete if not applicable): open
- Aeration: Continuous, membrane pump
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 100 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks on result:
- other: 95% CL, Significant level 0.05
- Results with reference substance (positive control):
- Deviation between respiration rates of the controls, 14.5%
Relevant effect levels: EC50 (3 h): 12.2 mg/L, NOEC < 5.0 mg/L - Reported statistics and error estimates:
- Determination of the EC50 of the reference item 3,5-dichlorophenol was accomplished using Probit-analysis (Finney-method, lognormal distribution, confidence limit 95%, significance level: 0.05).
- Validity criteria fulfilled:
- yes
- Conclusions:
- A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for CTO in a test conducted according to OECD 209. The result is considered reliable.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- See attached read-across justification report
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- other: EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks on result:
- other: 95% CL, Significant level 0.05
- Conclusions:
- A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for Tall Oil, Polymd. oxidized, based on
the result of the source study (see read across justification report). - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-04-24 to 2009-04-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: Different laoding rates (5 - 100 mg/l)
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Laboratory culture:
- Pretreatment: The activated sludge was washed three times with tap water and filtered using paper filter
- Initial biomass concentration: The recommended amount of wet sludge for test vessels was calculated to obtain a suspended solids level of 4 g/L (±10%) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 19.9 - 20.9 °C
- Dissolved oxygen:
- The preparations (control 1, 1 test loading rate of the test item, reference item in 3 test concentrations, control 2) were made up in 15 minute intervals and were aerated continuously so that a dissolved oxygen level of ≥ 6.5 mg/L was achieved during the whole test duration.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Control 1, 1 test loading rate of test item, reference item in 3 test concentrations, control 2.
- Type (delete if not applicable): open
- Aeration: Continuous, membrane pump
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 100 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks on result:
- other: 95% CL, Significant level 0.05
- Results with reference substance (positive control):
- Deviation between respiration rates of the controls, 14.5%
Relevant effect levels: Yes: Deviation between respiration rates of the controls, 14.5% - Reported statistics and error estimates:
- Determination of the EC50 of the reference item 3,5-dichlorophenol was accomplished using Probit-analysis (Finney-method, lognormal distribution, confidence limit 95%, significance level: 0.05).
- Validity criteria fulfilled:
- yes
- Conclusions:
- A Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for CTO in a test conducted according to OECD 209. The result is considered reliable.
Referenceopen allclose all
Table2:
Results of the Activated Sludge, Respiration Inhibition Test of CTO Sample 5
c / Loading rate (mg/L) |
Respiration rate (mg O2/L x h) |
Inhibition (%) |
|
Control 1 | - | 53.3 | - |
Reference | 3 | 47.1 | 18.6 |
Reference | 10 | 36.4 | 37.1 |
Reference | 30 | 12.8 | 77.9 |
Test item | 100 | 70.6 | -22.1* |
Control 2 | - | 62.3 | - |
Remark: * The negative inhibition value indicates, that the respiration rate was higher compared to the control.
Table2:
Results of the Activated Sludge, Respiration Inhibition Test of CTO Sample 3
c / Loading rate (mg/L) |
Respiration rate (mg O2/L x h) |
Inhibition (%) |
|
Control 1 | - | 53.3 | - |
Reference | 3 | 47.1 | 18.6 |
Reference | 10 | 36.4 | 37.1 |
Reference | 30 | 12.8 | 77.9 |
Test item | 5 | 55.8 | 3.5 |
Test item |
10 |
64.9 |
-12.2* |
Test item |
25 |
75.0 |
-29.7* |
Test item |
50 |
69.6 |
-20.3* |
Test item |
100 |
70.6 |
-22.1* |
Control 2 | - | 62.3 | - |
Remark: * Negative inhibition values indicate, that respiration rates were higher compared to the control. Taking into account the variation of results in the control and at the test loading rates of CTO Sample 3 the result of inhibition of 3.5 % at test loading rate of 5 mg/L is not regarded as a definite inhibition.
Description of key information
Water Accommodated Fractions (WAFs) of two CTO Samples caused no acute toxic effects on bacteria in an Activated Sludge, Respiration Inhibition Test as Limit Test conducted according to OECD 209. A 3 h EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined.
Based on the result of the source studies, a Water Accommodated Fractions (WAFs) value EL50 of > 100 mg/L and a NOELR of 100 mg/L have been determined for Tall Oil, Polymd. oxidized (see read across justification report).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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